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@proceedings{877567,
author = {Foltynová, Pavla and Peš, Ondřej and Vaculovič, Tomáš and Navrátilová, Jarmila and Šmarda, Jan and Kanický, Viktor and Preisler, Jan},
booktitle = {25th International Symposium on MicroScale Bioseparations MSB 2010},
keywords = {off-line coupling; ICP MS; SALD; mass spectrometry; proteins; biological samples},
language = {eng},
isbn = {978-80-254-6631-5},
title = {Preparation of microsamples for elemental analysis of biological samples},
year = {2010}
}
TY - CONF
ID - 877567
AU - Foltynová, Pavla - Peš, Ondřej - Vaculovič, Tomáš - Navrátilová, Jarmila - Šmarda, Jan - Kanický, Viktor - Preisler, Jan
PY - 2010
TI - Preparation of microsamples for elemental analysis of biological samples
SN - 9788025466315
KW - off-line coupling
KW - ICP MS
KW - SALD
KW - mass spectrometry
KW - proteins
KW - biological samples
N2 - New approaches of sample introduction to inductively-coupled plasma mass spectrometry which are especially suitable for minute amount of samples are presented. Detailed characterization of SALD ICP MS has shown large signal stability over a wide range of laser power density values, the independence of signal on acquisition modes and linear dynamic range over six orders of magnitude. SALD ICP MS with low limits of detection (0.1-14 fmol for Cr, Cu, Co, Fe, Ni, Sn and Zn) is suitable for off-line coupling with capillary electrophoresis (CE) for speciation analysis or selective detection of metals. Applicability to biological samples analysis was demonstrated on investigation of the role of copper ions to regulate cytotoxicity of disulfiram in myeloid leukemia cells. SALD ICP MS allows rapid, sensitive and reproducible method for quantitative analysis of metals with very low consumption of sample. The advantages of SALD are also sample archiving and possibility of consequent analysis in different detection modes, such as MALDI MS or fluorescence).
ER -
FOLTYNOVÁ, Pavla, Ondřej PEŠ, Tomáš VACULOVIČ, Jarmila NAVRÁTILOVÁ, Jan ŠMARDA, Viktor KANICKÝ a Jan PREISLER. Preparation of microsamples for elemental analysis of biological samples. In \textit{25th International Symposium on MicroScale Bioseparations MSB 2010}. 2010. ISBN~978-80-254-6631-5.
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