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@article{879411, author = {Salazar, Lisa and Kashiwada, Tamara and Krejčí, Pavel and Muchowski, Paul and Donoghue, Daniel and Wilcox, William and Thompson, Leslie Michels}, article_number = {11}, keywords = {GAMMA-INDEPENDENT PATHWAYS; PHOSPHATIDYLINOSITOL 3-KINASE; SIGNALING PATHWAY; FGFR3 MUTANTS; HEMATOPOIETIC TRANSFORMATION; THERAPEUTIC TARGET; TUMOR PROGRESSION; DOCKING PROTEIN; CANCER CELLS; PC12 CELLS}, language = {eng}, issn = {0964-6906}, journal = {HUMAN MOLECULAR GENETICS}, title = {A novel interaction between fibroblast growth factor receptor 3 and the p85 subunit of phosphoinositide 3-kinase: activation-dependent regulation of ERK by p85 in multiple myeloma cells}, volume = {18}, year = {2008} }
TY - JOUR ID - 879411 AU - Salazar, Lisa - Kashiwada, Tamara - Krejčí, Pavel - Muchowski, Paul - Donoghue, Daniel - Wilcox, William - Thompson, Leslie Michels PY - 2008 TI - A novel interaction between fibroblast growth factor receptor 3 and the p85 subunit of phosphoinositide 3-kinase: activation-dependent regulation of ERK by p85 in multiple myeloma cells JF - HUMAN MOLECULAR GENETICS VL - 18 IS - 11 SP - 1951-1961 EP - 1951-1961 SN - 09646906 KW - GAMMA-INDEPENDENT PATHWAYS KW - PHOSPHATIDYLINOSITOL 3-KINASE KW - SIGNALING PATHWAY KW - FGFR3 MUTANTS KW - HEMATOPOIETIC TRANSFORMATION KW - THERAPEUTIC TARGET KW - TUMOR PROGRESSION KW - DOCKING PROTEIN KW - CANCER CELLS KW - PC12 CELLS N2 - Ectopic activation of fibroblast growth factor receptor 3 (FGFR3) is associated with several cancers, including multiple myeloma (MM). FGFR3 inhibition in these cells inhibits proliferation and induces apoptosis, validating FGFR3 signaling as a therapeutic target in t(4;14) MM cases. We have identified the PI3K regulatory subunit, p85 alpha, as a novel interactor of FGFR3 by yeast two-hybrid, and confirmed an interaction with both p85 alpha and p85 beta in mammalian cells. The interaction of FGFR3 with p85 is dependent upon receptor activation. In contrast to the Gab1-mediated association of FGFRs with p85, the FGFR3-p85 interaction we observed requires FGFR3 Y760, previously identified as a PLC gamma binding site. The interaction of p85 with FGFR3 does not require PLC gamma, suggesting the p85 interaction is direct and independent of PLC gamma binding. FGFR3 and p85 proteins also interact in MM cell lines which consistently express p85 alpha and p85 beta, but not p50 or p55 subunits. siRNA knockdown of p85 beta in MM cells caused an increased ERK response to FGF2. These data suggest that an endogenous negative regulatory role for the p85-FGFR3 interaction on the Ras/ERK/MAPK pathway may exist in response to FGFR3 activity and identifies a novel therapeutic target for MM. ER -
SALAZAR, Lisa, Tamara KASHIWADA, Pavel KREJČÍ, Paul MUCHOWSKI, Daniel DONOGHUE, William WILCOX a Leslie Michels THOMPSON. A novel interaction between fibroblast growth factor receptor 3 and the p85 subunit of phosphoinositide 3-kinase: activation-dependent regulation of ERK by p85 in multiple myeloma cells. \textit{HUMAN MOLECULAR GENETICS}. 2008, roč.~18, č.~11, s.~1951-1961. ISSN~0964-6906.
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