Modulation of gp130 Signalling by Inhibitors of Reactive Oxygen
Species Formation in Mouse Embryonic Stem Cells

J 2009

Modulation of gp130 Signalling by Inhibitors of Reactive Oxygen Species Formation in Mouse Embryonic Stem Cells

KUČERA, Jan; Lukáš KUBALA; Hana KOTASOVÁ; Kateřina ŠTEFKOVÁ; Jiřina PROCHÁZKOVÁ et al.

Základní údaje

Originální název

Modulation of gp130 Signalling by Inhibitors of Reactive Oxygen Species Formation in Mouse Embryonic Stem Cells

Autoři

KUČERA, Jan; Lukáš KUBALA; Hana KOTASOVÁ; Kateřina ŠTEFKOVÁ; Jiřina PROCHÁZKOVÁ a Jiří PACHERNÍK

Vydání

FREE RADICAL BIOLOGY AND MEDICINE, New York, NY, USA, ELSEVIER SCIENCE INC, 2009, 0891-5849

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30105 Physiology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 6.081

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/09:00030039

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000271988500041

Klíčová slova anglicky

stem cells; gp130; reactive oxgen species; ROS; hypoxia; redox modulators;

Příznaky

Mezinárodní význam

Změněno: 10. 2. 2012 14:26, Mgr. Jiřina Medalová, Ph.D.

Anotace

V originále

The gp130 system is a set of ligands and receptors that dominantly involves the activation of the JAK/STAT, Erk, and PI3K/Akt pathways. gp130 is suggested to play a key role in response of stem cells to both the mediators that maintain stem cell undifferentiated phenotype and the mediators inducing differentiation. Under physiological conditions in their niche, stem cells are kept in hypoxic microenvironment. Leaving their niche they are exposed to normal levels of oxygen. Thus reactive oxygen species (ROS) formation in stem cells due to hypoxic conditions could be considered as important regulator of stem cell fate. Here we analyzed effects of inhibitors of NADPH oxidases apocynin or diphenyleneiodonium (DPI), and a glutathione precursor N-acetyl-cysteine (NAC) on activity of gp130 signaling pathways and maintenance of mouse ES cells. The obtained results are compared with effects of ES cells culture in hypoxia condition.

Návaznosti

GA301/08/0717, projekt VaV

Název: Regulace diferenciace ES buněk prostřednictvím receptoru gp130

Investor: Grantová agentura ČR, Regulace diferenciace ES buněk prostřednictvím receptoru gp130

GD204/09/H058, projekt VaV

Název: Mezibuněčná signalizace ve vývoji organismu a vzniku onemocnění

Investor: Grantová agentura ČR, Mezibuněčná signalizace ve vývoji organismu a vzniku onemocnění

Citovat

KUČERA, Jan; Lukáš KUBALA; Hana KOTASOVÁ; Kateřina ŠTEFKOVÁ; Jiřina PROCHÁZKOVÁ a Jiří PACHERNÍK. Modulation of gp130 Signalling by Inhibitors of Reactive Oxygen Species Formation in Mouse Embryonic Stem Cells. FREE RADICAL BIOLOGY AND MEDICINE. New York, NY, USA: ELSEVIER SCIENCE INC, 2009, roč. 47, zima, s. 18-19. ISSN 0891-5849.

@article{879419,
   author = {Kučera, Jan and Kubala, Lukáš and Kotasová, Hana and Štefková, Kateřina and Procházková, Jiřina and Pacherník, Jiří},
   article_location = {New York, NY, USA},
   article_number = {zima},
   keywords = {stem cells; gp130; reactive oxgen species; ROS; hypoxia; redox modulators;},
   language = {eng},
   issn = {0891-5849},
   journal = {FREE RADICAL BIOLOGY AND MEDICINE},
   title = {Modulation of gp130 Signalling by Inhibitors of Reactive Oxygen Species Formation in Mouse Embryonic Stem Cells},
   volume = {47},
   year = {2009}
}

TY  - JOUR
ID  - 879419
AU  - Kučera, Jan - Kubala, Lukáš - Kotasová, Hana - Štefková, Kateřina - Procházková, Jiřina - Pacherník, Jiří
PY  - 2009
TI  - Modulation of gp130 Signalling by Inhibitors of Reactive Oxygen Species Formation in Mouse Embryonic Stem Cells
JF  - FREE RADICAL BIOLOGY AND MEDICINE
VL  - 47
IS  - zima
SP  - 18
EP  - 18
PB  - ELSEVIER SCIENCE INC
SN  - 08915849
KW  - stem cells
KW  - gp130
KW  - reactive oxgen species
KW  - ROS
KW  - hypoxia
KW  - redox modulators;
N2  - The gp130 system is a set of ligands and receptors that dominantly involves the activation of the JAK/STAT, Erk, and PI3K/Akt pathways. gp130 is suggested to play a key role in response of stem cells to both the mediators that maintain stem cell undifferentiated phenotype and the mediators inducing differentiation. Under physiological conditions in their niche, stem cells are kept in hypoxic microenvironment. Leaving their niche they are exposed to normal levels of oxygen. Thus reactive oxygen species (ROS) formation in stem cells due to hypoxic conditions could be considered as important regulator of stem cell fate. Here we analyzed effects of inhibitors of NADPH oxidases apocynin or diphenyleneiodonium (DPI), and a glutathione precursor N-acetyl-cysteine (NAC) on activity of gp130 signaling pathways and maintenance of mouse ES cells. The obtained results are compared with effects of ES cells culture in hypoxia condition.
ER  -

KUČERA, Jan; Lukáš KUBALA; Hana KOTASOVÁ; Kateřina ŠTEFKOVÁ; Jiřina PROCHÁZKOVÁ a Jiří PACHERNÍK. Modulation of gp130 Signalling by Inhibitors of Reactive Oxygen Species Formation in Mouse Embryonic Stem Cells. \textit{FREE RADICAL BIOLOGY AND MEDICINE}. New York, NY, USA: ELSEVIER SCIENCE INC, 2009, roč.~47, zima, s.~18-19. ISSN~0891-5849.

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