Peripheral neuroactive steroids may be as good as the steroids
in the cerebrospinal fluid for the diagnostics of CNS
disturbances

J 2010

Peripheral neuroactive steroids may be as good as the steroids in the cerebrospinal fluid for the diagnostics of CNS disturbances

KANCHEVA, Radmila, Martin HILL, Zdeněk NOVÁK, Jan CHRASTINA, Marta VELIKOVA et. al.

Základní údaje

Originální název

Peripheral neuroactive steroids may be as good as the steroids in the cerebrospinal fluid for the diagnostics of CNS disturbances

Název česky

Periferní neuroaktivní steroidy mohou být stejně přínosné jako steroidy v mozkomíšním moku pro diagnostiku onemocnění centrálního nervového systému

Autoři

KANCHEVA, Radmila (203 Česká republika, garant), Martin HILL (203 Česká republika), Zdeněk NOVÁK (203 Česká republika, domácí), Jan CHRASTINA (203 Česká republika, domácí), Marta VELIKOVA (203 Česká republika), Lyudmila KANCHEVA (203 Česká republika), Ivo ŘÍHA (203 Česká republika, domácí) a Luboslav STARKA (203 Česká republika)

Vydání

Journal of Steroid Biochemistry and Molecular Biology, England, Elsevier Science, 2010, 0960-0760

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.886

Kód RIV

RIV/00216224:14110/10:00051346

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.jsbmb.2009.12.006

UT WoS

000276259500004

Klíčová slova česky

Steroidy; Mozkomíšní mok (CSF); Plasma; Metabolome; GC-MS.

Klíčová slova anglicky

Steroids; Cerebrospinal fluid (CSF); Plasma; Metabolome; GC-MS

Příznaky

Mezinárodní význam

Změněno: 16. 4. 2012 08:09, Mgr. Michal Petr

Anotace

ORIG CZ

V originále

To compare the predictivity of the neuroactive steroids in the cerebrospinal fluid and peripheral blood for the diagnostics of CNS disturbances, eighteen unconjugated steroids were quantified in the cerebrospinal fluid (CSF) from the 3rd ventricle and 18 unconjugated steroids and 7 steroid polar conjugates were measured in the serum using GC-MS and RIA. Eight postmenopausal women (56-78 years of age) and 7 men (22-88 years of age) with hydrocephalus were enrolled in the study. The sensitivity of the method ranged from low femtogram to low picogram levels depending on the steroid fragmentation pattern. Using multivariate regression, a model for simultaneous prediction of the CSF steroids from the serum steroids was completed. Then, the penetrability of the individual steroids across the blood-brain-barrier was evaluated and the sources of various brain steroids were estimated. Our data show that a part of the steroids may be synthesized de novo in the CNS. However, substantial part of the steroid metabolites may be synthesized in the CNS from the steroid precursors or directly transported from the periphery. The CNS in situ synthesis and transport from periphery might be complementary in some cases, i.e. brain synthesis might provide minimum level of steroids, which are indispensable for the CNS functions.

Česky

Získaná data ukazují, že část steroidů může být syntetizována de novo v centrálním nervovém systému. Ovšem podstatná část steroidních metabolitů může být v CNS syntezována ze steroidních prekurzorů nebo přímo transportována z periferie. Syntéza v CN a transport z periferie se mohou v určitých případech doplňovat, například mozková synteza by mohla poskytnout minimální hladiny steroidů, která je nutná pro funkci mozku.

Citovat

KANCHEVA, Radmila, Martin HILL, Zdeněk NOVÁK, Jan CHRASTINA, Marta VELIKOVA, Lyudmila KANCHEVA, Ivo ŘÍHA a Luboslav STARKA. Peripheral neuroactive steroids may be as good as the steroids in the cerebrospinal fluid for the diagnostics of CNS disturbances. Journal of Steroid Biochemistry and Molecular Biology. England: Elsevier Science, 2010, roč. 119, 1-2, s. 35-44. ISSN 0960-0760. Dostupné z: https://dx.doi.org/10.1016/j.jsbmb.2009.12.006.

@article{880129,
   author = {Kancheva, Radmila and Hill, Martin and Novák, Zdeněk and Chrastina, Jan and Velikova, Marta and Kancheva, Lyudmila and Říha, Ivo and Starka, Luboslav},
   article_location = {England},
   article_number = {1-2},
   doi = {http://dx.doi.org/10.1016/j.jsbmb.2009.12.006},
   keywords = {Steroids; Cerebrospinal fluid (CSF); Plasma; Metabolome; GC-MS},
   language = {eng},
   issn = {0960-0760},
   journal = {Journal of Steroid Biochemistry and Molecular Biology},
   title = {Peripheral neuroactive steroids may be as good as the steroids in the cerebrospinal fluid for the diagnostics of CNS disturbances},
   volume = {119},
   year = {2010}
}

TY  - JOUR
ID  - 880129
AU  - Kancheva, Radmila - Hill, Martin - Novák, Zdeněk - Chrastina, Jan - Velikova, Marta - Kancheva, Lyudmila - Říha, Ivo - Starka, Luboslav
PY  - 2010
TI  - Peripheral neuroactive steroids may be as good as the steroids in the cerebrospinal fluid for the diagnostics of CNS disturbances
JF  - Journal of Steroid Biochemistry and Molecular Biology
VL  - 119
IS  - 1-2
SP  - 35-44
EP  - 35-44
PB  - Elsevier Science
SN  - 09600760
KW  - Steroids
KW  - Cerebrospinal fluid (CSF)
KW  - Plasma
KW  - Metabolome
KW  - GC-MS
N2  - To compare the predictivity of the neuroactive steroids in the cerebrospinal fluid and peripheral blood for the diagnostics of CNS disturbances, eighteen unconjugated steroids were quantified in the cerebrospinal fluid (CSF) from the 3rd ventricle and 18 unconjugated steroids and 7 steroid polar conjugates were measured in the serum using GC-MS and RIA. Eight postmenopausal women (56-78 years of age) and 7 men (22-88 years of age) with hydrocephalus were enrolled in the study. The sensitivity of the method ranged from low femtogram to low picogram levels depending on the steroid fragmentation pattern. Using multivariate regression, a model for simultaneous prediction of the CSF steroids from the serum steroids was completed. Then, the penetrability of the individual steroids across the blood-brain-barrier was evaluated and the sources of various brain steroids were estimated. Our data show that a part of the steroids may be synthesized de novo in the CNS. However, substantial part of the steroid metabolites may be synthesized in the CNS from the steroid precursors or directly transported from the periphery. The CNS in situ synthesis and transport from periphery might be complementary in some cases, i.e. brain synthesis might provide minimum level of steroids, which are indispensable for the CNS functions.
ER  -

KANCHEVA, Radmila, Martin HILL, Zdeněk NOVÁK, Jan CHRASTINA, Marta VELIKOVA, Lyudmila KANCHEVA, Ivo ŘÍHA a Luboslav STARKA. Peripheral neuroactive steroids may be as good as the steroids in the cerebrospinal fluid for the diagnostics of CNS disturbances. \textit{Journal of Steroid Biochemistry and Molecular Biology}. England: Elsevier Science, 2010, roč.~119, 1-2, s.~35-44. ISSN~0960-0760. Dostupné z: https://dx.doi.org/10.1016/j.jsbmb.2009.12.006.

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