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@inproceedings{884980,
author = {Benešík, Martin and Janda, Lubomír and Dopitová, Radka and Doškař, Jiří and Pantůček, Roman},
address = {Brno},
booktitle = {XIV. Setkání biochemiků a molekulárních biologů. Sborník příspěvků},
edition = {1. vydání},
keywords = {bacteriophage; Staphylococcus aureus; bacteriolysis; lytic enzymes; protein preparetion},
language = {eng},
location = {Brno},
isbn = {978-80-210-5164-5},
pages = {45-46},
publisher = {Masarykova univerzita},
title = {Cloning and expression of endolysin and its SH3 domain from polyvalent staphylococcal bacteriophage},
url = {http://orion.chemi.muni.cz/Setkani/index.htm},
year = {2010}
}
TY - JOUR
ID - 884980
AU - Benešík, Martin - Janda, Lubomír - Dopitová, Radka - Doškař, Jiří - Pantůček, Roman
PY - 2010
TI - Cloning and expression of endolysin and its SH3 domain from polyvalent staphylococcal bacteriophage
PB - Masarykova univerzita
CY - Brno
SN - 9788021051645
KW - bacteriophage
KW - Staphylococcus aureus
KW - bacteriolysis
KW - lytic enzymes
KW - protein preparetion
UR - http://orion.chemi.muni.cz/Setkani/index.htm
N2 - Endolysins are phage lytic proteins which digest the cell wall of bacteria. Here we report expression of the genes encoding lytic proteins from bacteriophage 812. This enzymes lyses cells of Staphylococcus aureus and could have practical use in phage therapy. Activity of lytic enzymes was tested on different S. aureus strains that are insensitive to wild-type phage 812 and are resistant to antibiotics. The enzymes were active against a broader range of bacterial strains than the phage itself. Lytic protein of phage 812 includes three domains. SH3-domain is probably cell wall targeting domain, which could be responsible for binding of endolysin to peptidoglycan. SH3b-domain of phage 812 has been cloned and expressed as a soluble protein in E. coli. Protein was purified to homogeneity using ammonium sulphate precipitation, anion exchange chromatography, cation exchange chromatography, and gel filtration.
ER -
BENEŠÍK, Martin, Lubomír JANDA, Radka DOPITOVÁ, Jiří DOŠKAŘ a Roman PANTŮČEK. Cloning and expression of endolysin and its SH3 domain from polyvalent staphylococcal bacteriophage. In \textit{XIV. Setkání biochemiků a molekulárních biologů. Sborník příspěvků}. 1. vydání. Brno: Masarykova univerzita, 2010, s.~45-46. ISBN~978-80-210-5164-5.
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