Crohn's disease activity versus extent of DNA damage/repair and
variability in the RAGE gene

J 2010

Crohn's disease activity versus extent of DNA damage/repair and variability in the RAGE gene

PÁCAL, Lukáš, Jana VARVAŘOVSKÁ, Josef SÝKORA, Jana KOŽELUHOVÁ, Zdeněk RUŠAVÝ et. al.

Základní údaje

Originální název

Crohn's disease activity versus extent of DNA damage/repair and variability in the RAGE gene

Autoři

PÁCAL, Lukáš (203 Česká republika, garant), Jana VARVAŘOVSKÁ (203 Česká republika), Josef SÝKORA (203 Česká republika), Jana KOŽELUHOVÁ (203 Česká republika), Zdeněk RUŠAVÝ (203 Česká republika), Jaroslav RACEK (203 Česká republika), Rudolf ŠTĚTINA (203 Česká republika) a Kateřina KAŇKOVÁ (203 Česká republika)

Vydání

Scripta Medica, Brno, Masaryk University, Faculty of Medicine, 2010, 1211-3395

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14110/10:00040533

Organizační jednotka

Lékařská fakulta

Klíčová slova česky

AGEs; Crohn; glykoxidace; nutrigenetika; RAGE

Klíčová slova anglicky

AGEs; Crohn; glycoxidation; nutrigenetics; RAGE

Změněno: 23. 8. 2010 12:16, prof. MUDr. Kateřina Kaňková, Ph.D.

Anotace

V originále

The aim of the study was to investigate relationships between the extent of oxidative stress and DNA damage/repair, disease severity and selected genetic variants in the RAGE gene in Crohns disease (CD) patients. Study comprised a total of 46 subjects with CD and 99 control subjects. Disease activity was characterised by Crohn Disease Activity Index (CDAI) and pediatric CDAI (PCDAI) and complications requiring surgery (abscess, fistula or stenosis). Selected markers of oxidative stress (superoxide dismutase (Ery-SOD), glutathione peroxidise (WB-GPx), total plasma antioxidant capacity (P-tAOC), reduced glutathione (Ery-GSH) and malondialdehyde (P-MDA)), DNA damage (DNA single strand breaks) and repair capacity detected (DNArc) by Comet assay were ascertained. Four SNPs in the RAGE gene were detected by PCR. Significant correlation between CD duration and DNArc was identified in adult patients (r = 0.44, P = 0.039) and adult CD subjects had significantly higher Ery-SOD levels (P = 0.0005). In children, both CDAI and PCDAI significantly correlated with P-tAOC (r = -0.5, P = 0.02 and r = -0.53, P = 0.013, respectively) and also with WB-GPx (r = -0.5, P = 0.03 and r = -0.51, P = 0.018, respectively). Children CD patients also exhibited significant correlation between age and Ery-GSH and P-MDA (r = -0.6, P = 0.005 and r = 0.5, P = 0.02, respectively). Significant differences in Ery-GSH and P-MDA were found between CD patients with and without history of complications (P = 0.05 and P = 0.013, respectively). There was no statistically significant relationship between the carrier state of any of the four RAGE SNPs and ox. stress, DNA damage or DNA repair parameters analysed. In conclusion, intestinal inflammation is reflected in selected circulating markers of oxidative and genotoxic stress.

Návaznosti

KJB501620601, projekt VaV

Název: Funkční analýza rizikového haplotypu RAGE genu a jeho role v patogenezi hyperglykemií indukovaných změn

Investor: Akademie věd ČR, Funkční analýza rizikového haplotypu RAGE genu a jeho role v patogenezi hyperglykemií indukovaných změn

Citovat

PÁCAL, Lukáš, Jana VARVAŘOVSKÁ, Josef SÝKORA, Jana KOŽELUHOVÁ, Zdeněk RUŠAVÝ, Jaroslav RACEK, Rudolf ŠTĚTINA a Kateřina KAŇKOVÁ. Crohn's disease activity versus extent of DNA damage/repair and variability in the RAGE gene. Scripta Medica. Brno: Masaryk University, Faculty of Medicine, 2010, roč. 83/2010, č. 1, s. 72-80. ISSN 1211-3395.

@article{886810,
   author = {Pácal, Lukáš and Varvařovská, Jana and Sýkora, Josef and Koželuhová, Jana and Rušavý, Zdeněk and Racek, Jaroslav and Štětina, Rudolf and Kaňková, Kateřina},
   article_location = {Brno},
   article_number = {1},
   keywords = {AGEs; Crohn; glycoxidation; nutrigenetics; RAGE},
   language = {eng},
   issn = {1211-3395},
   journal = {Scripta Medica},
   title = {Crohn's disease activity versus extent of DNA damage/repair and variability in the RAGE gene},
   volume = {83/2010},
   year = {2010}
}

TY  - JOUR
ID  - 886810
AU  - Pácal, Lukáš - Varvařovská, Jana - Sýkora, Josef - Koželuhová, Jana - Rušavý, Zdeněk - Racek, Jaroslav - Štětina, Rudolf - Kaňková, Kateřina
PY  - 2010
TI  - Crohn's disease activity versus extent of DNA damage/repair and variability in the RAGE gene
JF  - Scripta Medica
VL  - 83/2010
IS  - 1
SP  - 72-80
EP  - 72-80
PB  - Masaryk University, Faculty of Medicine
SN  - 12113395
KW  - AGEs
KW  - Crohn
KW  - glycoxidation
KW  - nutrigenetics
KW  - RAGE
N2  - The aim of the study was to investigate relationships between the extent of oxidative stress and DNA damage/repair, disease severity and selected genetic variants in the RAGE gene in Crohns disease (CD) patients. Study comprised a total of 46 subjects with CD and 99 control subjects. Disease activity was characterised by Crohn Disease Activity Index (CDAI) and pediatric CDAI (PCDAI) and complications requiring surgery (abscess, fistula or stenosis). Selected markers of oxidative stress (superoxide dismutase (Ery-SOD), glutathione peroxidise (WB-GPx), total plasma antioxidant capacity (P-tAOC), reduced glutathione (Ery-GSH) and malondialdehyde (P-MDA)), DNA damage (DNA single strand breaks) and repair capacity detected (DNArc) by Comet assay were ascertained. Four SNPs in the RAGE gene were detected by PCR. Significant correlation between CD duration and DNArc was identified in adult patients (r = 0.44, P = 0.039) and adult CD subjects had significantly higher Ery-SOD levels (P = 0.0005). In children, both CDAI and PCDAI significantly correlated with P-tAOC (r = -0.5, P = 0.02 and r = -0.53, P = 0.013, respectively) and also with WB-GPx (r = -0.5, P = 0.03 and r = -0.51, P = 0.018, respectively). Children CD patients also exhibited significant correlation between age and Ery-GSH and P-MDA (r = -0.6, P = 0.005 and r = 0.5, P = 0.02, respectively). Significant differences in Ery-GSH and P-MDA were found between CD patients with and without history of complications (P = 0.05 and P = 0.013, respectively). There was no statistically significant relationship between the carrier state of any of the four RAGE SNPs and ox. stress, DNA damage or DNA repair parameters analysed. In conclusion, intestinal inflammation is reflected in selected circulating markers of oxidative and genotoxic stress.
ER  -

PÁCAL, Lukáš, Jana VARVAŘOVSKÁ, Josef SÝKORA, Jana KOŽELUHOVÁ, Zdeněk RUŠAVÝ, Jaroslav RACEK, Rudolf ŠTĚTINA a Kateřina KAŇKOVÁ. Crohn's disease activity versus extent of DNA damage/repair and variability in the RAGE gene. \textit{Scripta Medica}. Brno: Masaryk University, Faculty of Medicine, 2010, roč.~83/2010, č.~1, s.~72-80. ISSN~1211-3395.

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