Proteomics uncovers extreme heterogeneity in the Staphylococcus
aureus exoproteome due to genomic plasticity and variant gene
regulation

J 2010

Proteomics uncovers extreme heterogeneity in the Staphylococcus aureus exoproteome due to genomic plasticity and variant gene regulation

ZIEBANDT, Anne-Kathrin; Harald KUSCH; Marco DEGNER; Sarah JAGLITZ; Mark J. J. B. SIBBALD et al.

Základní údaje

Originální název

Proteomics uncovers extreme heterogeneity in the Staphylococcus aureus exoproteome due to genomic plasticity and variant gene regulation

Autoři

Vydání

Proteomics, Weinheim, Wiley-VCH, 2010, 1615-9853

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.815

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/10:00044086

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000277309800008

Klíčová slova anglicky

Exoproteome; Microbiology; Protein secretion; Staphylococcus; TOXIC-SHOCK-SYNDROME; METHICILLIN-RESISTANT; BACILLUS-SUBTILIS; SIGNAL-TRANSDUCTION; VIRULENCE FACTORS; NASAL CARRIAGE

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 6. 2010 17:35, prof. RNDr. Roman Pantůček, Ph.D.

Anotace

ORIG CZ

V originále

Sequencing of at least 13 Staphylococcus aureus isolates has shown that genomic plasticity impacts significantly on the repertoire of virulence factors. However, genome sequencing does not reveal which genes are expressed by individual isolates. Here, we have therefore performed a comprehensive survey of the composition and variability of the S. aureus exoproteome. This involved multilocus sequence typing, virulence gene, and prophage profiling by multiplex PCR, and proteomic analyses of secreted proteins using 2-DE. Dissection of the exoproteomes of 25 clinical isolates revealed that only seven out of 63 identified secreted proteins were produced by all isolates, indicating a remarkably high exoproteome heterogeneity within one bacterial species. Most interesting, the observed variations were caused not only by genome plasticity, but also by an unprecedented variation in secretory protein production due to differences in transcriptional and post-transcriptional regulation. Our data imply that genomic studies on virulence gene conservation patterns need to be complemented by analyses of the extracellular protein pattern to assess the full virulence potential of bacterial pathogens like S. aureus. Importantly, the extensive variability of secreted virulence factors in S. aureus also suggests that development of protective vaccines against this pathogen requires a carefully selected combination of invariably produced antigens.

Česky

Byla provedena podrobná charakterizace složení a variability exoproteomu Staphylococcus aureus. Analýzy zahrnovala typizaci izolátů prostřednictvím MLST, identifikace genů virulence, profágového profilování multiplex PCR, a proteomické analýzy 2-DE. Disekce exoproteomu 25 klinických izolátů odhalila, že pouze 7 z 63 proteinů bylo produkovaných všemi kmeny, což naznačuje extrémní heterogenitu uvnitř bakteriálního druhu.

Návaznosti

MSM0021622415, záměr

Název: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární podstata buněčných a tkáňových regulací

Citovat

ZIEBANDT, Anne-Kathrin; Harald KUSCH; Marco DEGNER; Sarah JAGLITZ; Mark J. J. B. SIBBALD; Jan P. ARENDS; Monika A. CHLEBOWICZ; Dirk ALBRECHT; Roman PANTŮČEK; Jiří DOŠKAŘ; Wilma ZIEBUHR; Barbara M. BRÖKER; Michael HECKER; Jan Maarten VAN DIJL a Susanne ENGELMANN. Proteomics uncovers extreme heterogeneity in the Staphylococcus aureus exoproteome due to genomic plasticity and variant gene regulation. Proteomics. Weinheim: Wiley-VCH, 2010, roč. 10, č. 8, s. 1634-1644. ISSN 1615-9853.

@article{886928,
   author = {Ziebandt, AnneandKathrin and Kusch, Harald and Degner, Marco and Jaglitz, Sarah and Sibbald, Mark J. J. B. and Arends, Jan P. and Chlebowicz, Monika A. and Albrecht, Dirk and Pantůček, Roman and Doškař, Jiří and Ziebuhr, Wilma and Bröker, Barbara M. and Hecker, Michael and van Dijl, Jan Maarten and Engelmann, Susanne},
   article_location = {Weinheim},
   article_number = {8},
   keywords = {Exoproteome; Microbiology; Protein secretion; Staphylococcus; TOXIC-SHOCK-SYNDROME; METHICILLIN-RESISTANT; BACILLUS-SUBTILIS; SIGNAL-TRANSDUCTION; VIRULENCE FACTORS; NASAL CARRIAGE},
   language = {eng},
   issn = {1615-9853},
   journal = {Proteomics},
   title = {Proteomics uncovers extreme heterogeneity in the Staphylococcus aureus exoproteome due to genomic plasticity and variant gene regulation},
   url = {http://www3.interscience.wiley.com/journal/123299148/abstract},
   volume = {10},
   year = {2010}
}

TY  - JOUR
ID  - 886928
AU  - Ziebandt, Anne-Kathrin - Kusch, Harald - Degner, Marco - Jaglitz, Sarah - Sibbald, Mark J. J. B. - Arends, Jan P. - Chlebowicz, Monika A. - Albrecht, Dirk - Pantůček, Roman - Doškař, Jiří - Ziebuhr, Wilma - Bröker, Barbara M. - Hecker, Michael - van Dijl, Jan Maarten - Engelmann, Susanne
PY  - 2010
TI  - Proteomics uncovers extreme heterogeneity in the Staphylococcus aureus exoproteome due to genomic plasticity and variant gene regulation
JF  - Proteomics
VL  - 10
IS  - 8
SP  - 1634-1644
EP  - 1634-1644
PB  - Wiley-VCH
SN  - 16159853
KW  - Exoproteome
KW  - Microbiology
KW  - Protein secretion
KW  - Staphylococcus
KW  - TOXIC-SHOCK-SYNDROME
KW  - METHICILLIN-RESISTANT
KW  - BACILLUS-SUBTILIS
KW  - SIGNAL-TRANSDUCTION
KW  - VIRULENCE FACTORS
KW  - NASAL CARRIAGE
UR  - http://www3.interscience.wiley.com/journal/123299148/abstract
N2  - Sequencing of at least 13 Staphylococcus aureus isolates has shown that genomic plasticity impacts significantly on the repertoire of virulence factors. However, genome sequencing does not reveal which genes are expressed by individual isolates. Here, we have therefore performed a comprehensive survey of the composition and variability of the S. aureus exoproteome. This involved multilocus sequence typing, virulence gene, and prophage profiling by multiplex PCR, and proteomic analyses of secreted proteins using 2-DE. Dissection of the exoproteomes of 25 clinical isolates revealed that only seven out of 63 identified secreted proteins were produced by all isolates, indicating a remarkably high exoproteome heterogeneity within one bacterial species. Most interesting, the observed variations were caused not only by genome plasticity, but also by an unprecedented variation in secretory protein production due to differences in transcriptional and post-transcriptional regulation. Our data imply that genomic studies on virulence gene conservation patterns need to be complemented by analyses of the extracellular protein pattern to assess the full virulence potential of bacterial pathogens like S. aureus. Importantly, the extensive variability of secreted virulence factors in S. aureus also suggests that development of protective vaccines against this pathogen requires a carefully selected combination of invariably produced antigens.
ER  -

ZIEBANDT, Anne-Kathrin; Harald KUSCH; Marco DEGNER; Sarah JAGLITZ; Mark J. J. B. SIBBALD; Jan P. ARENDS; Monika A. CHLEBOWICZ; Dirk ALBRECHT; Roman PANTŮČEK; Jiří DOŠKAŘ; Wilma ZIEBUHR; Barbara M. BRÖKER; Michael HECKER; Jan Maarten VAN DIJL a Susanne ENGELMANN. Proteomics uncovers extreme heterogeneity in the Staphylococcus aureus exoproteome due to genomic plasticity and variant gene regulation. \textit{Proteomics}. Weinheim: Wiley-VCH, 2010, roč.~10, č.~8, s.~1634-1644. ISSN~1615-9853.

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