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@proceedings{887888,
author = {Dejmková, Eva and Pokorná, Martina and Alán, Jan and Wimmerová, Michaela},
booktitle = {XIV. Setkání biochemiků a molekulárních biologů},
keywords = {Lectin; Chromobacterium violaceum; role of a water molecule},
language = {eng},
isbn = {978-80-210-5164-5},
title = {Lectin from Chromobacterium violaceum - role of water molecule in the carbohydrate binding site},
year = {2010}
}
TY - CONF
ID - 887888
AU - Dejmková, Eva - Pokorná, Martina - Alán, Jan - Wimmerová, Michaela
PY - 2010
TI - Lectin from Chromobacterium violaceum - role of water molecule in the carbohydrate binding site
SN - 9788021051645
KW - Lectin
KW - Chromobacterium violaceum
KW - role of a water molecule
N2 - CV-IIL is a lectin from human opprotunistic pathogen microorganism Chromobacterium violaceum. Crystal structure of this lectin demonstrates, that there is one water molecule in the carbohydrate binding site, which plays a special role by bridging sugar with backbone nitrogen atom and also with side chain of the amino acid threonine in position 97. Substitution of this amino acid should lead to changes of thermodynamical parametres (an enthalpic and entropic contribution to the free energy of the interaction lectin-sugar) that could be determine by an isothermal titration microcalorimetry method.
ER -
DEJMKOVÁ, Eva, Martina POKORNÁ, Jan ALÁN a Michaela WIMMEROVÁ. Lectin from Chromobacterium violaceum - role of water molecule in the carbohydrate binding site. In \textit{XIV. Setkání biochemiků a molekulárních biologů}. 2010. ISBN~978-80-210-5164-5.
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