Clotting factors and eicosanoids protect against nematode
infections

a 2010

Clotting factors and eicosanoids protect against nematode infections

HYRŠL, Pavel; Pavel DOBEŠ; Zhi WANG a Ulrich THEOPOLD

Základní údaje

Originální název

Clotting factors and eicosanoids protect against nematode infections

Autoři

HYRŠL, Pavel (203 Česká republika, garant, domácí); Pavel DOBEŠ (203 Česká republika); Zhi WANG (156 Čína) a Ulrich THEOPOLD (752 Švédsko)

Vydání

43rd Annual Meeting SIP 2010 Turkey, 2010

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30102 Immunology

Stát vydavatele

Turecko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/10:00048742

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000285568700009

Klíčová slova česky

Drosophila; Heterorhabditis; imunita; srážení hemolymfy; eikosanoidy

Klíčová slova anglicky

Drosophila; Heterorhabditis; immunity; clotting; eicosanoids

Příznaky

Mezinárodní význam

Změněno: 4. 1. 2011 10:47, Mgr. Pavel Dobeš, Ph.D.

Anotace

V originále

Entomopathogenic nematodes (EPNs) of the genera Heterorhabditis are obligate and lethal insect parasites. In recent years they have been used increasingly as biological control agents. Infective juveniles occur free living in the soil and are capable of seeking out hosts and penetrate them through the cuticle or natural orifices. EPNs are symbiotically associated with bacteria of the genera Photorhabdus. The bacterial symbionts are essential to kill the host (within 24-48 hours) and digest host tissues. Drosophila larvae are more resistant to nematode infection than Galleria mellonella, but both can be used as natural infection model. The tripartite model (Drosophila, nematodes, bacteria) was recently established and used to show an immune function for transglutaminase, a conserved clotting factor. In this study we used different Drosophila mutants or RNAi lines with defects in clotting or other branches of the immune system. We demonstrated an immune function during nematode infection for known clotting substrates GP150 and Fondue. Our experiments show that compared to control animals Imd and Bc mutant larvae have similar viability after infection. In contrast double mutants in Imd and Bc show significantly higher mortality suggesting that phenoloxidase cooperates with the Imd pathway during the response to the nematobacterial complex. Furthermore, injection of eicosanoid biosynthesis inhibitors increases susceptibility to nematodes implying the importance of eicosanoids. In conclusion, we show that the Heterorhabditis/Photorhabdus infection model is suitable to identify novel regulators of innate immunity. Our research is supported by grants from the Carl-Tryggers Foundation (U.T.) and Grant Agency of Czech Republic (GA206/09/P470).

Návaznosti

GP206/09/P470, projekt VaV

Název: Vliv inhibitorů biosyntézy eikosanoidů na imunitu zavíječe voskového Galleria mellonella

Investor: Grantová agentura ČR, Vliv inhibitorů biosyntézy eikonsanoidů na imunitu zavíječe voskového Galleria mellonella

Citovat

HYRŠL, Pavel; Pavel DOBEŠ; Zhi WANG a Ulrich THEOPOLD. Clotting factors and eicosanoids protect against nematode infections. In 43rd Annual Meeting SIP 2010 Turkey. 2010.

@proceedings{891797,
   author = {Hyršl, Pavel and Dobeš, Pavel and Wang, Zhi and Theopold, Ulrich},
   booktitle = {43rd Annual Meeting SIP 2010 Turkey},
   keywords = {Drosophila; Heterorhabditis; immunity; clotting; eicosanoids},
   language = {eng},
   title = {Clotting factors and eicosanoids protect against nematode infections},
   year = {2010}
}

TY  - CONF
ID  - 891797
AU  - Hyršl, Pavel - Dobeš, Pavel - Wang, Zhi - Theopold, Ulrich
PY  - 2010
TI  - Clotting factors and eicosanoids protect against nematode infections
KW  - Drosophila
KW  - Heterorhabditis
KW  - immunity
KW  - clotting
KW  - eicosanoids
N2  - Entomopathogenic nematodes (EPNs) of the genera Heterorhabditis are obligate and lethal insect parasites. In recent years they have been used increasingly as biological control agents. Infective juveniles occur free living in the soil and are capable of seeking out hosts and penetrate them through the cuticle or natural orifices. EPNs are symbiotically associated with bacteria of the genera Photorhabdus. The bacterial symbionts are essential to kill the host (within 24-48 hours) and digest host tissues. Drosophila larvae are more resistant to nematode infection than Galleria mellonella, but both can be used as natural infection model. The tripartite model (Drosophila, nematodes, bacteria) was recently established and used to show an immune function for transglutaminase, a conserved clotting factor. In this study we used different Drosophila mutants or RNAi lines with defects in clotting or other branches of the immune system. We demonstrated an immune function during nematode infection for known clotting substrates GP150 and Fondue. Our experiments show that compared to control animals Imd and Bc mutant larvae have similar viability after infection. In contrast double mutants in Imd and Bc show significantly higher mortality suggesting that phenoloxidase cooperates with the Imd pathway during the response to the nematobacterial complex. Furthermore, injection of eicosanoid biosynthesis inhibitors increases susceptibility to nematodes implying the importance of eicosanoids. In conclusion, we show that the Heterorhabditis/Photorhabdus infection model is suitable to identify novel regulators of innate immunity. Our research is supported by grants from the Carl-Tryggers Foundation (U.T.) and Grant Agency of Czech Republic (GA206/09/P470).
ER  -

HYRŠL, Pavel; Pavel DOBEŠ; Zhi WANG a Ulrich THEOPOLD. Clotting factors and eicosanoids protect against nematode infections. In \textit{43rd Annual Meeting SIP 2010 Turkey}. 2010.

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