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@article{893205,
author = {Žaludová, Renata and Žákovská, Alena and Kašpárková, Jana and Balcarová, Zdeňka and Kleinwächter, Vladimír and Vrána, Oldřich and Farrell, Nicholas and Brabec, Viktor},
article_number = {2},
keywords = {DNA; platinum drug; dinuclear complex; cross-link; anticancer effect},
language = {eng},
issn = {0014-2956},
journal = {EUROPEAN JOURNAL OF BIOCHEMISTRY},
title = {DNA interactions of bifunctional dinuclear platinum(II) antitumor agents},
volume = {246},
year = {1997}
}
TY - JOUR
ID - 893205
AU - Žaludová, Renata - Žákovská, Alena - Kašpárková, Jana - Balcarová, Zdeňka - Kleinwächter, Vladimír - Vrána, Oldřich - Farrell, Nicholas - Brabec, Viktor
PY - 1997
TI - DNA interactions of bifunctional dinuclear platinum(II) antitumor agents
JF - EUROPEAN JOURNAL OF BIOCHEMISTRY
VL - 246
IS - 2
SN - 00142956
KW - DNA
KW - platinum drug
KW - dinuclear complex
KW - cross-link
KW - anticancer effect
N2 - Modifications of natural DNA in a cell-free medium by dinuclear bisplatinum complexes with equivalent coordination spheres, represented by the general formula [{trans-PtCl(NH3)(2)}(2)(H2N-R-NH2)](2+), where R is a propane or hexane, were studied by various methods of biochemical analysis or molecular biophysics. These methods include binding studies by means of differential-pulse polarography, measurements of melting curves with the aid of absorption spectrophotometry, measurements of CD spectra, ELISA with specific antibodies that recognize DNA modified by platinum complexes, interstrand crosslinking assay employing gel electrophoresis under denaturing conditions and mapping of DNA adducts by means of transcription assays. The results indicated that the major adduct of [{trans-PtCl(NH3)(2)}(2)(H2N-R-NH2)](2+) in DNA was an interstrand cross-link which was formed with a relatively short half-time (approximate to 1 h). At least some types of these interstrand cross-links induced local denaturational changes in the DNA.
ER -
ŽALUDOVÁ, Renata, Alena ŽÁKOVSKÁ, Jana KAŠPÁRKOVÁ, Zdeňka BALCAROVÁ, Vladimír KLEINWÄCHTER, Oldřich VRÁNA, Nicholas FARRELL a Viktor BRABEC. DNA interactions of bifunctional dinuclear platinum(II) antitumor agents. \textit{EUROPEAN JOURNAL OF BIOCHEMISTRY}. 1997, roč.~246, č.~2, 10 s. ISSN~0014-2956.
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