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@article{893209,
author = {Brabec, Viktor and Boudný, Vladimír and Balcarová, Zdeňka},
article_number = {6},
keywords = {ANTITUMOR DRUG CIS-DIAMMINEDICHLOROPLATINUM(II); RNA-POLYMERASES; D(AG) SITE; COMPLEXES; BINDING; CONFORMATION; SPECIFICITY; SINGLE; D(GG)},
language = {eng},
issn = {0006-2960},
journal = {BIOCHEMISTRY},
title = {MONOFUNCTIONAL ADDUCTS OF PLATINUM(II) PRODUCE IN DNA A SEQUENCE-DEPENDENT LOCAL DENATURATION},
volume = {33},
year = {1997}
}
TY - JOUR
ID - 893209
AU - Brabec, Viktor - Boudný, Vladimír - Balcarová, Zdeňka
PY - 1997
TI - MONOFUNCTIONAL ADDUCTS OF PLATINUM(II) PRODUCE IN DNA A SEQUENCE-DEPENDENT LOCAL DENATURATION
JF - BIOCHEMISTRY
VL - 33
IS - 6
SN - 00062960
KW - ANTITUMOR DRUG CIS-DIAMMINEDICHLOROPLATINUM(II)
KW - RNA-POLYMERASES
KW - D(AG) SITE
KW - COMPLEXES
KW - BINDING
KW - CONFORMATION
KW - SPECIFICITY
KW - SINGLE
KW - D(GG)
N2 - The effects on the conformation of DNA produced by the monofunctional adducts of chloro(diethylenetriamine)platinum(II) chloride or cis-diamminemonoaquamonochloroplatinum(II) have been investigated by means of the single-strand-specific probe chloroacetaldehyde (CAA). The denatured sites to which CAA was bound and that were induced in DNA by the monofunctional adducts of the platinum complexes were characterized by means of three experimental approaches. These include measurement of the fluorescence of a plasmid fragment treated with CAA, analysis of oligonucleotides treated with CAA and cleaved by piperidine, and termination of duplex transcription on a fragment of plasmid DNA treated with CAA. The results indicate that the denaturational change preferentially occurs in the base pair containing the monoadducted deoxyriboguanosine in the trinucleotide sequence Py-deoxyriboguanosine-Py (Py is a pyrimidine deoxyribonucleoside). It was suggested that this conformational alteration facilitates in DNA the formation of minor bifunctional adducts of cis-diamminedichloroplatinum(II).
ER -
BRABEC, Viktor, Vladimír BOUDNÝ a Zdeňka BALCAROVÁ. MONOFUNCTIONAL ADDUCTS OF PLATINUM(II) PRODUCE IN DNA A SEQUENCE-DEPENDENT LOCAL DENATURATION. \textit{BIOCHEMISTRY}. 1997, roč.~33, č.~6, 7 s. ISSN~0006-2960.
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