1997
MONOFUNCTIONAL ADDUCTS OF PLATINUM(II) PRODUCE IN DNA A SEQUENCE-DEPENDENT LOCAL DENATURATION
BRABEC, Viktor, Vladimír BOUDNÝ a Zdeňka BALCAROVÁZákladní údaje
Originální název
MONOFUNCTIONAL ADDUCTS OF PLATINUM(II) PRODUCE IN DNA A SEQUENCE-DEPENDENT LOCAL DENATURATION
Autoři
BRABEC, Viktor, Vladimír BOUDNÝ a Zdeňka BALCAROVÁ
Vydání
BIOCHEMISTRY, 1997, 0006-2960
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10600 1.6 Biological sciences
Stát vydavatele
Spojené státy
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 4.572
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova anglicky
ANTITUMOR DRUG CIS-DIAMMINEDICHLOROPLATINUM(II); RNA-POLYMERASES; D(AG) SITE; COMPLEXES; BINDING; CONFORMATION; SPECIFICITY; SINGLE; D(GG)
Změněno: 5. 8. 2010 14:48, RNDr. Kamila Neplechová, Ph.D.
Anotace
V originále
The effects on the conformation of DNA produced by the monofunctional adducts of chloro(diethylenetriamine)platinum(II) chloride or cis-diamminemonoaquamonochloroplatinum(II) have been investigated by means of the single-strand-specific probe chloroacetaldehyde (CAA). The denatured sites to which CAA was bound and that were induced in DNA by the monofunctional adducts of the platinum complexes were characterized by means of three experimental approaches. These include measurement of the fluorescence of a plasmid fragment treated with CAA, analysis of oligonucleotides treated with CAA and cleaved by piperidine, and termination of duplex transcription on a fragment of plasmid DNA treated with CAA. The results indicate that the denaturational change preferentially occurs in the base pair containing the monoadducted deoxyriboguanosine in the trinucleotide sequence Py-deoxyriboguanosine-Py (Py is a pyrimidine deoxyribonucleoside). It was suggested that this conformational alteration facilitates in DNA the formation of minor bifunctional adducts of cis-diamminedichloroplatinum(II).