CLEAVAGE BY RESTRICTION ENZYMES OF DNA MODIFIED WITH THE ANTITUMOR DRUG CIS-DIAMMINEDICHLOROPLATINUM(II)

BALCAROVÁ, Zdeňka, Jan MRÁZEK, Vladimír KLEINWÄCHTER a Viktor BRABEC. CLEAVAGE BY RESTRICTION ENZYMES OF DNA MODIFIED WITH THE ANTITUMOR DRUG CIS-DIAMMINEDICHLOROPLATINUM(II). GENERAL PHYSIOLOGY AND BIOPHYSICS. 1992, roč. 11, č. 6, 10 s. ISSN 0231-5882.

Základní údaje

Originální název

CLEAVAGE BY RESTRICTION ENZYMES OF DNA MODIFIED WITH THE ANTITUMOR DRUG CIS-DIAMMINEDICHLOROPLATINUM(II)

Autoři

BALCAROVÁ, Zdeňka, Jan MRÁZEK, Vladimír KLEINWÄCHTER a Viktor BRABEC

Vydání

GENERAL PHYSIOLOGY AND BIOPHYSICS, 1992, 0231-5882

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Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10610 Biophysics

Stát vydavatele

Slovensko

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

PLATINUM COMPLEXES; PLASMID DNA; RESTRICTION ENDONUCLEASES; GEL ELECTROPHORESIS

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Anotace

V originále

The effect of binding of an antitumour drug cis-diamminedichloroplatinum(II) (cis-[Pt(NH3)2Cl2]) to DNA on cutting effectiveness of BAMHI, EcoRI, and SalI restriction endonucleases was quantitatively determined. The platinum complex inhibits the cleavage of plasmid pHC624 DNA linearized by BglI restrictase. From the present results we conclude that the yield of restriction endonuclease cleavage is also lowered if the platinum complex is bound outside the recognition DNA sequence of these enzymes. We propose that the origin of platinum adducts on DNA outside the recognition sequence can decrease the yield of restriction enzyme cleavage via inducing a conformational perturbation in the recognition DNA sequence of these enzymes and also via inhibition of the linear diffusion of these enzymes on DNA.