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@article{896912,
author = {Souček, Přemysl and Klíma, Petr and Reková, Alena and Brzobohatý, Břetislav},
article_location = {Oxford},
article_number = {13},
keywords = {regulation of gene expression; knox genes; pop/lhgr system; Arabidopsis thaliana; plant hormones},
language = {eng},
issn = {0022-0957},
journal = {Journal of Experimental Botany},
title = {Involvement of hormones and KNOXI genes in early Arabidopsis seedling development},
url = {http://jxb.oxfordjournals.org/archive},
volume = {58},
year = {2007}
}
TY - JOUR
ID - 896912
AU - Souček, Přemysl - Klíma, Petr - Reková, Alena - Brzobohatý, Břetislav
PY - 2007
TI - Involvement of hormones and KNOXI genes in early Arabidopsis seedling development
JF - Journal of Experimental Botany
VL - 58
IS - 13
SP - 3797-3810
EP - 3797-3810
PB - Oxford University Press
SN - 00220957
KW - regulation of gene expression
KW - knox genes
KW - pop/lhgr system
KW - Arabidopsis thaliana
KW - plant hormones
UR - http://jxb.oxfordjournals.org/archive
N2 - Plant hormones control plant development by modulating the expression of regulatory genes, including homeobox-containing KNOXI genes. However, much remains to be elucidated about the interactions involved. Therefore, hormonal regulation of KNOXI gene expression was investigated using hormone applications and an inducible transgenic ipt expression system to increase endogenous cytokinin (CK) levels. Treatments with auxin, abscisic acid (ABA), cytokinins, ethylene, and gibberellin (GA) did not result in ectopic expression of the BP (BREVIPEDICELLUS) gene. However, BP expression was strongly reduced by ABA, increased by auxin treatment (correlating with the initiation of lateral root meristems, which strongly express BP), and did not significantly respond to short-term treatments with the other hormones in whole seedlings. Following short-term ipt activation, organ-specific differential regulation of KNOXI gene expression was observed. While several KNOXI genes were transiently up-regulated to low levels, STM was selectively repressed (especially at low light) in hypocotyls. In cotyledons, activation of CK-responsive genes preceded ipt induction, suggesting that CKs are transported more rapidly than the inducing agent (dexamethasone). Long-term increases in CK levels induced raised levels of several KNOXI transcripts in hypocotyls, correlating with the radial expansion of vascular tissues, the main domains of KNOXI gene expression, suggesting that CKs had little effect on KNOXI promoter activity. No alterations in hormone sensitivity were observed in a bp null mutant. Constitutive BP overexpression caused reductions in the length and number of lateral roots, while the primary root remained unaffected. The transgenic seedlings displayed weak, but significant, alterations in sensitivity to ABA, CK, and 1-amino-cyclopropane-1-carboxylic acid.
ER -
SOUČEK, Přemysl, Petr KLÍMA, Alena REKOVÁ a Břetislav BRZOBOHATÝ. Involvement of hormones and KNOXI genes in early Arabidopsis seedling development. \textit{Journal of Experimental Botany}. Oxford: Oxford University Press, 2007, roč.~58, č.~13, s.~3797-3810. ISSN~0022-0957.
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