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@proceedings{898271,
author = {Crha, Igor and Přibyl, Jan and Skládal, Petr and Žáková, Jana and Ventruba, Pavel and Pohanka, Michal},
booktitle = {26th Annual Meeting of ESHRE},
keywords = {sperm; AFM; infertility},
language = {eng},
note = {HUMAN REPRODUCTION Volume: 25 Supplement: 1 Pages: I122-I122},
title = {Determination of the surface pathology of human sperm by atomic force microscopy},
year = {2010}
}
TY - CONF
ID - 898271
AU - Crha, Igor - Přibyl, Jan - Skládal, Petr - Žáková, Jana - Ventruba, Pavel - Pohanka, Michal
PY - 2010
TI - Determination of the surface pathology of human sperm by atomic force microscopy
N1 - HUMAN REPRODUCTION Volume: 25 Supplement: 1 Pages: I122-I122
KW - sperm
KW - AFM
KW - infertility
N2 - Atomic force microscopy (AFM) employs a sharp probe for profiling surfaces with unique resolution. This technique has developed into an invaluable multidisciplinary tool for advanced characterization of different samples. In its basic application, AFM provides high resolution imaging of surface structures at scales ranging from a few nanometers to hundreds micrometers. Oxidative stress is considered one of the basic etiological factors of the male’s fertility disorders. Excessive production of oxygen radicals results in damage to the sperm’s cell membrane and to the DNA. The collected AFM images clearly highlight many details of normal spermatozoa and spermatozoa damaged by hydrogen peroxide. This technique could be an important tool in the research of oxidative stress and understanding its effect on male infertility.
ER -
CRHA, Igor, Jan PŘIBYL, Petr SKLÁDAL, Jana ŽÁKOVÁ, Pavel VENTRUBA a Michal POHANKA. Determination of the surface pathology of human sperm by atomic force microscopy. In \textit{26th Annual Meeting of ESHRE}. 2010. ISSN~0268-1161.
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