ZEISBERGEROVÁ, Marta, Roman ŘEMÍNEK, Aleš MÁDR, Zdeněk GLATZ, Jos HOOGMARTENS a Ann VAN SCHEPDAEL. On-line drug metabolites generation and their subsequent target analysis by capillary zone electrophoresis with UV-absorption detection. Electrophoresis. Weinheim: WILEY-VCH Verlag GmbH, 2010, roč. 31, č. 19, s. 3256-3262. ISSN 0173-0835. |
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@article{898963, author = {Zeisbergerová, Marta and Řemínek, Roman and Mádr, Aleš and Glatz, Zdeněk and Hoogmartens, Jos and Van Schepdael, Ann}, article_location = {Weinheim}, article_number = {19}, keywords = {CE; Cytochrome P450; Dextromethorphan; Metabolism;}, language = {eng}, issn = {0173-0835}, journal = {Electrophoresis}, title = {On-line drug metabolites generation and their subsequent target analysis by capillary zone electrophoresis with UV-absorption detection}, volume = {31}, year = {2010} }
TY - JOUR ID - 898963 AU - Zeisbergerová, Marta - Řemínek, Roman - Mádr, Aleš - Glatz, Zdeněk - Hoogmartens, Jos - Van Schepdael, Ann PY - 2010 TI - On-line drug metabolites generation and their subsequent target analysis by capillary zone electrophoresis with UV-absorption detection JF - Electrophoresis VL - 31 IS - 19 SP - 3256-3262 EP - 3256-3262 PB - WILEY-VCH Verlag GmbH SN - 01730835 KW - CE KW - Cytochrome P450 KW - Dextromethorphan KW - Metabolism; N2 - This study presents the in-capillary enzymatic biotransformation of dextromethorphan, an antitusive drug and opioid receptors antagonist, and subsequent electrophoretic separation of its products. The study includes the optimization of separation parameters to fulfill the requirements of an online microreaction. The analyses were performed in a bare fused-silica capillary using 100mM sodium tetraborate (pH 10.0) mixed with linear polyacrylamide (20%, v/v) and 2-propanol (10%, v/v). This BGE was suitable for monitoring both off-line and in-capillary incubations. The partial filling technique enabled the enzymatic reaction to be carried out in its optimal environment (20mM sodium phosphate, pH 7.4). Finally, in-capillary microreaction in the presence of cytochrome P450 3A4 gave satisfactory outcomes. ER -
ZEISBERGEROVÁ, Marta, Roman ŘEMÍNEK, Aleš MÁDR, Zdeněk GLATZ, Jos HOOGMARTENS a Ann VAN SCHEPDAEL. On-line drug metabolites generation and their subsequent target analysis by capillary zone electrophoresis with UV-absorption detection. \textit{Electrophoresis}. Weinheim: WILEY-VCH Verlag GmbH, 2010, roč.~31, č.~19, s.~3256-3262. ISSN~0173-0835.
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