Using the FISH method for an identification of genetic
alterations in cervical carcinoma ane cervical intraepithelial
neoplasia: gain of human telomerase gene (hTERC)

a 2010

Using the FISH method for an identification of genetic alterations in cervical carcinoma ane cervical intraepithelial neoplasia: gain of human telomerase gene (hTERC)

SLÁMOVÁ, Iva; Lucie MOUKOVÁ; Dita ŽEŽULKOVÁ; Nina ŠTROSSOVÁ; Vladimíra VRANOVÁ et al.

Základní údaje

Originální název

Using the FISH method for an identification of genetic alterations in cervical carcinoma ane cervical intraepithelial neoplasia: gain of human telomerase gene (hTERC)

Název česky

Využití metody FISH pro identifikaci genetických abnormalit u karcinomu děložního čípku a jeho prekanceróz: zisk genu pro lidskou telomerázu (hTERC)

Autoři

SLÁMOVÁ, Iva; Lucie MOUKOVÁ; Dita ŽEŽULKOVÁ; Nina ŠTROSSOVÁ; Vladimíra VRANOVÁ a Petr KUGLÍK

Vydání

Europen Human Genetics Conference, 2010

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

Genetika a molekulární biologie

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.380

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/10:00044840

Organizační jednotka

Přírodovědecká fakulta

ISSN

Klíčová slova česky

FISH, carcinom děložního čípku, zisk genu hTERC

Klíčová slova anglicky

FISH; cervical carcinoma; gain of hTERC

Příznaky

Mezinárodní význam

Změněno: 31. 1. 2012 12:00, RNDr. Vladimíra Vallová, Ph.D.

Anotace

V originále

Cervical cancer is the second most common cancer in women in the Czech Republic, approximately 400 women die of this disease every year. It is known that this carcinoma develops from precancerous cervical intraepithelial neoplasia (CIN) which is characterized by series of genetic abnormalities. Gain of hTERC (3q26) was the most consistent chromosomal abnormality found in cervical carcinoma. It was discovered that gain of hTERC gene is associated with progression from precansers (CIN1, CIN2, CIN3) to cervical carcinoma. In our work, we studied copy number changes of the chromosomal region of hTERC gene using the FISH method in cytological smears obtained from 30 patients of the Departement of Gynaecological Oncology of Masaryk Memorial Cancer Institute Brno. Gain of hTERC gene was found in 16 from 30 (53 %) patiens with the diagnosis of cervical cancer or with CIN1-CIN3. It was confirmed that gain of hTERC is significantly associated with cervical carcinoma. In 2009 a new DNA probe for simultaneous identification of HPV infection and examination of copy number changes of hTERC and MYCC genes using the FISH method was created by Abbott. The initial experiences with detection of hTERC and MYCC genes in HPV positive cells in CIN3 are presented. The results of genetic analyses should serve as a supporting tool for the determination of samples containing high risk HPV infections and chromosomal aberrations linked to the potential progression and tumor proliferation.

Návaznosti

MSM0021622415, záměr

Název: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární podstata buněčných a tkáňových regulací

Citovat

SLÁMOVÁ, Iva; Lucie MOUKOVÁ; Dita ŽEŽULKOVÁ; Nina ŠTROSSOVÁ; Vladimíra VRANOVÁ a Petr KUGLÍK. Using the FISH method for an identification of genetic alterations in cervical carcinoma ane cervical intraepithelial neoplasia: gain of human telomerase gene (hTERC). In Europen Human Genetics Conference. 2010. ISSN 1018-4813.

@proceedings{899079,
   author = {Slámová, Iva and Mouková, Lucie and Žežulková, Dita and Štrossová, Nina and Vranová, Vladimíra and Kuglík, Petr},
   booktitle = {Europen Human Genetics Conference},
   keywords = {FISH; cervical carcinoma; gain of hTERC},
   language = {eng},
   title = {Using the FISH method for an identification of genetic alterations in cervical carcinoma ane cervical intraepithelial neoplasia: gain of human telomerase gene (hTERC)},
   url = {http://www.eshg.org},
   year = {2010}
}

TY  - CONF
ID  - 899079
AU  - Slámová, Iva - Mouková, Lucie - Žežulková, Dita - Štrossová, Nina - Vranová, Vladimíra - Kuglík, Petr
PY  - 2010
TI  - Using the FISH method for an identification of genetic alterations in cervical carcinoma ane cervical intraepithelial neoplasia: gain of human telomerase gene (hTERC)
KW  - FISH
KW  - cervical carcinoma
KW  - gain of hTERC
UR  - http://www.eshg.org
N2  - Cervical cancer is the second most common cancer in women in the Czech Republic, approximately 400 women die of this disease every year. It is known that this carcinoma develops from precancerous cervical intraepithelial neoplasia (CIN) which is characterized by series of genetic abnormalities. Gain of hTERC (3q26) was the most consistent chromosomal abnormality found in cervical carcinoma. It was discovered that gain of hTERC gene is associated with progression from precansers (CIN1, CIN2, CIN3) to cervical carcinoma. In our work, we studied copy number changes of the chromosomal region of hTERC gene using the FISH method in cytological smears obtained from 30 patients of the Departement of Gynaecological Oncology of Masaryk Memorial Cancer Institute Brno. Gain of hTERC gene was found in 16 from 30 (53 %) patiens with the diagnosis of cervical cancer or with CIN1-CIN3. It was confirmed that gain of hTERC is significantly associated with cervical carcinoma. In 2009 a new DNA probe for simultaneous identification of HPV infection and examination of copy number changes of hTERC and MYCC genes using the FISH method was created by Abbott. The initial experiences with detection of hTERC and MYCC genes in HPV positive cells in CIN3 are presented. The results of genetic analyses should serve as a supporting tool for the determination of samples containing high risk HPV infections and chromosomal aberrations linked to the potential progression and tumor proliferation.
ER  -

SLÁMOVÁ, Iva; Lucie MOUKOVÁ; Dita ŽEŽULKOVÁ; Nina ŠTROSSOVÁ; Vladimíra VRANOVÁ a Petr KUGLÍK. Using the FISH method for an identification of genetic alterations in cervical carcinoma ane cervical intraepithelial neoplasia: gain of human telomerase gene (hTERC). In \textit{Europen Human Genetics Conference}. 2010. ISSN~1018-4813.

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