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@inproceedings{900386,
author = {Černý, Martin and Brzobohatý, Břetislav},
address = {Brno},
edition = {1},
location = {Brno},
isbn = {978-80-7375-239-2},
publisher = {MZLU},
title = {Isolation of Phosphoproteom and its Application in Study of the Effect of Cytokinin on Plants},
url = {http://web2.mendelu.cz/af_291_mendelnet/mendelnet08agro/files/articles/biolrost_cerny.pdf},
year = {2008}
}
TY - JOUR
ID - 900386
AU - Černý, Martin - Brzobohatý, Břetislav
PY - 2008
TI - Isolation of Phosphoproteom and its Application in Study of the Effect of Cytokinin on Plants
PB - MZLU
CY - Brno
SN - 9788073752392
UR - http://web2.mendelu.cz/af_291_mendelnet/mendelnet08agro/files/articles/biolrost_cerny.pdf
N2 - Phosphorylation is one of the most important posttranslational modifications in proteins. It is also a major issue in modern proteomics. Its application in plant research is rather scarce. We present here development of optimized protocol for phophoprotein enrichment kit (Quiagen) for extraction of plant phosphoproteom. This protocol allows reusing of affinity column designated for single use only up to 3 times with phosphoproteom quality unaffected. We also provide example of application of this improved protocol in research of cytokinin activation pathway in plants.
ER -
ČERNÝ, Martin a Břetislav BRZOBOHATÝ. \textit{Isolation of Phosphoproteom and its Application in Study of the Effect of Cytokinin on Plants}. 1. vyd. Brno: MZLU, 2008, 5 s. ISBN~978-80-7375-239-2.
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