| PROKOP, Zbyněk, Yukari SATO, Jan BREZOVSKÝ, Tomáš MOZGA, Radka CHALOUPKOVÁ, Táňa KOUDELÁKOVÁ, Petr JEŘÁBEK, Veronika ŠTĚPÁNKOVÁ, Rio NATSUME, J.G.E. LEEWEN, D,B. JANSSEN, J. FLORIAN, Yuji NAGATA, T. SENDA a Jiří DAMBORSKÝ. Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering. Angewandte Chemie International Edition. Wiley-VCH Verlag GmbH & Co. KGaA, Weinhe, roč. 49, č. 35, s. 6111-6115. ISSN 1433-7851. 2010. |
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@article{902208,
author = {Prokop, Zbyněk and Sato, Yukari and Brezovský, Jan and Mozga, Tomáš and Chaloupková, Radka and Koudeláková, Táňa and Jeřábek, Petr and Štěpánková, Veronika and Natsume, Rio and Leewen, J.G.E. and Janssen, D,B. and Florian, J. and Nagata, Yuji and Senda, T. and Damborský, Jiří},
article_number = {35},
keywords = {Enantioselectivity; Haloalkane Dehalogenases; protein engineering},
language = {eng},
issn = {1433-7851},
journal = {Angewandte Chemie International Edition},
title = {Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering},
url = {http://loschmidt.chemi.muni.cz/peg/pdf/angewandte2010.pdf},
volume = {49},
year = {2010}
}
TY - JOUR
ID - 902208
AU - Prokop, Zbyněk - Sato, Yukari - Brezovský, Jan - Mozga, Tomáš - Chaloupková, Radka - Koudeláková, Táňa - Jeřábek, Petr - Štěpánková, Veronika - Natsume, Rio - Leewen, J.G.E. - Janssen, D,B. - Florian, J. - Nagata, Yuji - Senda, T. - Damborský, Jiří
PY - 2010
TI - Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering
JF - Angewandte Chemie International Edition
VL - 49
IS - 35
SP - 6111-6115
EP - 6111-6115
PB - Wiley-VCH Verlag GmbH & Co. KGaA, Weinhe
SN - 14337851
KW - Enantioselectivity
KW - Haloalkane Dehalogenases
KW - protein engineering
UR - http://loschmidt.chemi.muni.cz/peg/pdf/angewandte2010.pdf
N2 - Engineering of the surface loop in haloalkane dehalogenases affects their enantiodiscrimination behavior. The temperature dependence of the enantioselectivity (lnE versus 1/T) of -bromoalkanes by haloalkane dehalogenases is reversed (red data points) by deletion of the surface loop; the selectivity switches back when an additional single-point mutation is made. This behavior is not observed for -bromoesters.
ER -
PROKOP, Zbyněk, Yukari SATO, Jan BREZOVSKÝ, Tomáš MOZGA, Radka CHALOUPKOVÁ, Táňa KOUDELÁKOVÁ, Petr JEŘÁBEK, Veronika ŠTĚPÁNKOVÁ, Rio NATSUME, J.G.E. LEEWEN, D,B. JANSSEN, J. FLORIAN, Yuji NAGATA, T. SENDA a Jiří DAMBORSKÝ. Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering. \textit{Angewandte Chemie International Edition}. Wiley-VCH Verlag GmbH \&{} Co. KGaA, Weinhe, roč.~49, č.~35, s.~6111-6115. ISSN~1433-7851. 2010.
|
