Biomarker discovery in low-grade breast cancer: An integrated,
proteomics-based approach

a 2010

Biomarker discovery in low-grade breast cancer: An integrated, proteomics-based approach

BOUCHAL, Pavel; Monika MUDROCHOVÁ; Zbyněk BORTLÍČEK; Theodoros ROUMELIOTIS; Eva BUDINSKÁ et al.

Základní údaje

Originální název

Biomarker discovery in low-grade breast cancer: An integrated, proteomics-based approach

Název česky

Vyhledávání biomarkerů low-grade karcinomu prsu: Integrovaný přístup na bázi proteomiky

Autoři

Vydání

Abstract book of "3rd joint meeting on the role of p53, MDM2, AGR2/3 and ubiquitin/chaperone system in tumour biology" 2010

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-86793-16-0

Klíčová slova česky

rakovina prsu; uzlinové metastáze proteomika

Klíčová slova anglicky

breast cancer; metastasis; proteomics

Příznaky

Mezinárodní význam

Změněno: 4. 5. 2011 12:14, doc. Mgr. Pavel Bouchal, Ph.D.

Anotace

V originále

Breast cancer is the most common women cancer in the world. Traditional prognostic markers are, however, not sufficient for precise risk-group discrimination. Our effort has been focused on biomarker discovery in the set of 96 breast cancer primary tumors divided into several groups according to grade, lymph node and hormonal receptor status. Three proteomics approaches were involved in complex protein analysis of tissue lysates: SELDI-TOF MS, iTRAQ-2DLC-MS/MS and 2-D SDS-PAGE. Generally, our results indicate the following differences between the two studied groups: Differential expression of (i) cytoskeletal proteins, (ii) AGR family members and (iii) proteins involved in heme biosynthesis. These findings provide a solid data for further verification, functional studies and validation geared toward the establishment of novel biomarkers of metastatic processes in breast cancer.

Návaznosti

GAP304/10/0868, projekt VaV

Název: Studium molekulárních mechanismů časného metastazování do lymfatických uzlin u karcinomu prsu nízkého stupně malignity pomocí proteomických technik

Investor: Grantová agentura ČR, Studium molekulárních mechanismů časného metastazování do lymfatických uzlin u karcinomu prsu nízkého stupně malignity pomocí proteomických technik

GA301/08/1468, projekt VaV

Název: Úloha E3-ligáz a inhibitorů heat shock proteinů při studiu molekulárních a buněčných konsekvencí regulace proteinu p53.

MZ0MOU2005, záměr

Název: Funkční diagnostika zhoubných nádorů

Citovat

BOUCHAL, Pavel; Monika MUDROCHOVÁ; Zbyněk BORTLÍČEK; Theodoros ROUMELIOTIS; Eva BUDINSKÁ; Dominika VÁŇOVÁ; Lenka HERNYCHOVÁ; Iva STRUHÁROVÁ; Roman HRSTKA; Petr MÜLLER; Alexander SCHERL; Spiros D. GARBIS; Rudolf NENUTIL a Bořivoj VOJTĚŠEK. Biomarker discovery in low-grade breast cancer: An integrated, proteomics-based approach. In Abstract book of "3rd joint meeting on the role of p53, MDM2, AGR2/3 and ubiquitin/chaperone system in tumour biology". 2010. ISBN 978-80-86793-16-0.

@proceedings{902367,
   author = {Bouchal, Pavel and Mudrochová, Monika and Bortlíček, Zbyněk and Roumeliotis, Theodoros and Budinská, Eva and Váňová, Dominika and Hernychová, Lenka and Struhárová, Iva and Hrstka, Roman and Müller, Petr and Scherl, Alexander and Garbis, Spiros D. and Nenutil, Rudolf and Vojtěšek, Bořivoj},
   booktitle = {Abstract book of "3rd joint meeting on the role of p53, MDM2, AGR2/3 and ubiquitin/chaperone system in tumour biology"},
   keywords = {breast cancer; metastasis; proteomics},
   language = {eng},
   isbn = {978-80-86793-16-0},
   title = {Biomarker discovery in low-grade breast cancer: An integrated, proteomics-based approach},
   year = {2010}
}

TY  - CONF
ID  - 902367
AU  - Bouchal, Pavel - Mudrochová, Monika - Bortlíček, Zbyněk - Roumeliotis, Theodoros - Budinská, Eva - Váňová, Dominika - Hernychová, Lenka - Struhárová, Iva - Hrstka, Roman - Müller, Petr - Scherl, Alexander - Garbis, Spiros D. - Nenutil, Rudolf - Vojtěšek, Bořivoj
PY  - 2010
TI  - Biomarker discovery in low-grade breast cancer: An integrated, proteomics-based approach
SN  - 9788086793160
KW  - breast cancer
KW  - metastasis
KW  - proteomics
N2  - Breast cancer is the most common women cancer in the world. Traditional prognostic markers are, however, not sufficient for precise risk-group discrimination. Our effort has been focused on biomarker discovery in the set of 96 breast cancer primary tumors divided into several groups according to grade, lymph node and hormonal receptor status. Three proteomics approaches were involved in complex protein analysis of tissue lysates: SELDI-TOF MS, iTRAQ-2DLC-MS/MS and 2-D SDS-PAGE. Generally, our results indicate the following differences between the two studied groups: Differential expression of (i) cytoskeletal proteins, (ii) AGR family members and (iii) proteins involved in heme biosynthesis. These findings provide a solid data for further verification, functional studies and validation geared toward the establishment of novel biomarkers of metastatic processes in breast cancer.
ER  -

BOUCHAL, Pavel; Monika MUDROCHOVÁ; Zbyněk BORTLÍČEK; Theodoros ROUMELIOTIS; Eva BUDINSKÁ; Dominika VÁŇOVÁ; Lenka HERNYCHOVÁ; Iva STRUHÁROVÁ; Roman HRSTKA; Petr MÜLLER; Alexander SCHERL; Spiros D. GARBIS; Rudolf NENUTIL a Bořivoj VOJTĚŠEK. Biomarker discovery in low-grade breast cancer: An integrated, proteomics-based approach. In \textit{Abstract book of ''3rd joint meeting on the role of p53, MDM2, AGR2/3 and ubiquitin/chaperone system in tumour biology''}. 2010. ISBN~978-80-86793-16-0.

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