| KRONTORÁD KOUTNÁ, Irena, Martina PETERKOVÁ, Pavel ŠIMARA, Stanislav STEJSKAL, Lenka TESAŘOVÁ a Michal KOZUBEK. Proliferation and differentiation potential of CD133+ and CD34+ populations from the bone marrow and mobilized peripheral blood. Annals of hematology. Germany: Springer, roč. 90, (2), s. 127-137. ISSN 0939-5555. 2011. |
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@article{907888,
author = {Krontorád Koutná, Irena and Peterková, Martina and Šimara, Pavel and Stejskal, Stanislav and Tesařová, Lenka and Kozubek, Michal},
article_location = {Germany},
article_number = {(2)},
keywords = {CD34; CD133; HSC; transplantation; VEGFR},
language = {eng},
issn = {0939-5555},
journal = {Annals of hematology},
title = {Proliferation and differentiation potential of CD133+ and CD34+ populations from the bone marrow and mobilized peripheral blood},
volume = {90},
year = {2011}
}
TY - JOUR
ID - 907888
AU - Krontorád Koutná, Irena - Peterková, Martina - Šimara, Pavel - Stejskal, Stanislav - Tesařová, Lenka - Kozubek, Michal
PY - 2011
TI - Proliferation and differentiation potential of CD133+ and CD34+ populations from the bone marrow and mobilized peripheral blood
JF - Annals of hematology
VL - 90
IS - (2)
SP - 127-137
EP - 127-137
PB - Springer
SN - 09395555
KW - CD34
KW - CD133
KW - HSC
KW - transplantation
KW - VEGFR
N2 - CD34 is the most frequently used marker for the selection of cells for bone marrow (BM) transplantation. The use of CD133 as an alternative marker is an open research topic. The goal of this study was to evaluate the proliferation and differentiation potential for hematopoiesis (short and long term) of CD133+ and CD34+ populations from bone marrow and mobilized peripheral blood. Eight cell populations were compared: CD34+ and CD133+ cells from both the BM (CML Ph-, CML Ph+, and healthy volunteers) and mobilized peripheral blood cells. Multicolor flow cytometry and cultivation experiments were used to measure expression and differentiation of the individual populations. It was observed that the CD133+ BM population showed higher cell expansion. Another finding is that during a 6-day cultivation with 5(6)-carboxyfluorescein diacetate N- succinimidyl ester (CFSE), more cells remained in division D0 (non-dividing cells). There was a higher percentage of CD38- cells observed on the CD133+ BM population. It was also observed that the studied populations contained very similar but not the same pools of progenitors: erythroid, lymphoid, and myeloid. This was confirmed by CFU-GM and CFU-E experiments. The VEGFR antigen was used to monitor subpopulations of endothelial sinusoidal progenitors. The CD133+ BM population contained significantly more VEGFR+ cells. Our findings suggest that the CD133+ population from the BM shows better proliferation activity and a higher distribution of primitive progenitors than any other studied population.
ER -
KRONTORÁD KOUTNÁ, Irena, Martina PETERKOVÁ, Pavel ŠIMARA, Stanislav STEJSKAL, Lenka TESAŘOVÁ a Michal KOZUBEK. Proliferation and differentiation potential of CD133+ and CD34+ populations from the bone marrow and mobilized peripheral blood. \textit{Annals of hematology}. Germany: Springer, roč.~90, (2), s.~127-137. ISSN~0939-5555. 2011.
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