ŽERAVÍK, Jiří, Karel LACINA, Milan JÍLEK, Jiří VLČEK a Petr SKLÁDAL. Biosensor for determination of carboxylic acids in wines based on the inhibition of sarcosine oxidase. Microchimica Acta. Wien (Austria): Springer, 2010, roč. 170, 3-4, s. 251-256. ISSN 0026-3672. |
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@article{908995, author = {Žeravík, Jiří and Lacina, Karel and Jílek, Milan and Vlček, Jiří and Skládal, Petr}, article_location = {Wien (Austria)}, article_number = {3-4}, keywords = {Biosensor; Sarcosine oxidase; Carboxylic acid; Wine;}, language = {eng}, issn = {0026-3672}, journal = {Microchimica Acta}, title = {Biosensor for determination of carboxylic acids in wines based on the inhibition of sarcosine oxidase}, volume = {170}, year = {2010} }
TY - JOUR ID - 908995 AU - Žeravík, Jiří - Lacina, Karel - Jílek, Milan - Vlček, Jiří - Skládal, Petr PY - 2010 TI - Biosensor for determination of carboxylic acids in wines based on the inhibition of sarcosine oxidase JF - Microchimica Acta VL - 170 IS - 3-4 SP - 251-256 EP - 251-256 PB - Springer SN - 00263672 KW - Biosensor KW - Sarcosine oxidase KW - Carboxylic acid KW - Wine; N2 - The flow-through amperometric biosensor is presented for determination of carboxylic acids. It is based on two sensor layers that are deposited on a platinum electrode. The inner layer serves to eliminate interferences by limiting diffusion of electrochemically active substances such as ascorbic acid. This layer is electro-polymerized using an equimolar mixture of o-phenylenediamine and resorcinol. The outer layer is prepared by cross-linking the enzyme sarcosine oxidase and bovine serum albumin using glutaraldehyde. The formation of enzymatically produced hydrogen peroxide is monitored at 600 mV vs. an Ag/AgCl reference electrode. The addition of carboxylic acids causes competitive inhibition of the enzyme and a decrease in signal. The assay was optimized for determination of carboxylic acids in wine samples. Following 10-fold dilution, most samples contain 1-10 mM individual carboxylic acids and thus a 5 mM concentration of sarcosine was chosen as being optimal for competition. ER -
ŽERAVÍK, Jiří, Karel LACINA, Milan JÍLEK, Jiří VLČEK a Petr SKLÁDAL. Biosensor for determination of carboxylic acids in wines based on the inhibition of sarcosine oxidase. \textit{Microchimica Acta}. Wien (Austria): Springer, 2010, roč.~170, 3-4, s.~251-256. ISSN~0026-3672.
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