Effect of tetrahydroiso-alpha-acids on the growth of
beer-spoiling and –nonspoiling bacteria

J 2010

Effect of tetrahydroiso-alpha-acids on the growth of beer-spoiling and –nonspoiling bacteria

MATOULKOVÁ, Dagmar; Miroslav NĚMEC a Karel SIGLER

Základní údaje

Originální název

Effect of tetrahydroiso-alpha-acids on the growth of beer-spoiling and –nonspoiling bacteria

Název česky

Vliv tetrahydro-izo-alfa-hořkých kyselin na růst bakterií kazících a nekazících pivo

Autoři

MATOULKOVÁ, Dagmar; Miroslav NĚMEC a Karel SIGLER

Vydání

Kvasný Průmysl, Praha, 2010

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova česky

kažení piva, rezistence k hořkým chmelovým látkám, Lactobacillus brevis, tetrahydroizo-alfa-hořké kyseliny, růstové parametry, stres

Klíčová slova anglicky

beer spoilage, resistance to hop bitter compounds, Lactobacillus brevis, tetrahydroiso-alpha-acids, growth parameters, stress

Příznaky

Recenzováno

Změněno: 26. 11. 2010 08:06, RNDr. Dagmar Matoulková, Ph.D.

Anotace

V originále

We studied the effects of hop analogue compounds tetrahydroiso-alpha-acids (Tetra) on the growth and growth parameters of 23 Lactobacillus brevis strains isolated from several Czech lager breweries. Forcing test was used to divide the isolates into two groups, beer-spoilage strains (10 isolates) and non-spoilers (13 isolates). The growth of L. brevis in MRS-broth containing varying concentrations of Tetra was investigated using Bioscreen C. The measured growth curves were used to calculate the basic growth parameters. All 23 isolates were able to grow in the presence of tetrahydroiso-alpha-acids, albeit to a varying extent. Tetra affected the growth and multiplication of all L. brevis strains regardless of their beer-spoilage ability. Increasing concentrations of Tetra led to a decrease in cell suspension optical density (O.D.) and in the specific growth rate, and extension of lag phase. The results and their statistic analysis revealed no relationship between the beer-spoilage ability and hop-resistance. The degree of resistance to the antibacterial activity of Tetra was strongly affected by the initial pH of culture medium, but there was no correlation between the pH-induced change in the level of resistance and the beer spoilage ability of any L. brevis strain.

Návaznosti

MSM0021622413, záměr

Název: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Proteiny v metabolismu a při interakci organismů s prostředím

MSM6019369701, záměr

Název: Výzkum sladařských a pivovarských surovin a technologií

Citovat

MATOULKOVÁ, Dagmar; Miroslav NĚMEC a Karel SIGLER. Effect of tetrahydroiso-alpha-acids on the growth of beer-spoiling and –nonspoiling bacteria. Kvasný Průmysl. Praha, 2010, č. 10, s. 396-403.

@article{909079,
   author = {Matoulková, Dagmar and Němec, Miroslav and Sigler, Karel},
   article_location = {Praha},
   article_number = {10},
   keywords = {beer spoilage, resistance to hop bitter compounds, Lactobacillus brevis, tetrahydroiso-alpha-acids, growth parameters, stress},
   language = {eng},
   journal = {Kvasný Průmysl},
   title = {Effect of tetrahydroiso-alpha-acids on the growth of beer-spoiling and –nonspoiling bacteria},
   year = {2010}
}

TY  - JOUR
ID  - 909079
AU  - Matoulková, Dagmar - Němec, Miroslav - Sigler, Karel
PY  - 2010
TI  - Effect of tetrahydroiso-alpha-acids on the growth of beer-spoiling and –nonspoiling bacteria
JF  - Kvasný Průmysl
IS  - 10
SP  - 396-403
EP  - 396-403
KW  - beer spoilage, resistance to hop bitter compounds, Lactobacillus brevis, tetrahydroiso-alpha-acids, growth parameters, stress
N2  - We studied the effects of hop analogue compounds tetrahydroiso-alpha-acids (Tetra) on the growth and growth parameters of 23 Lactobacillus brevis strains isolated from several Czech lager breweries. Forcing test was used to divide the isolates into two groups, beer-spoilage strains (10 isolates) and non-spoilers (13 isolates). The growth of L. brevis in MRS-broth containing varying concentrations of Tetra was investigated using Bioscreen C. The measured growth curves were used to calculate the basic growth parameters. All 23 isolates were able to grow in the presence of tetrahydroiso-alpha-acids, albeit to a varying extent. Tetra affected the growth and multiplication of all L. brevis strains regardless of their beer-spoilage ability. Increasing concentrations of Tetra led to a decrease in cell suspension optical density (O.D.) and in the specific growth rate, and extension of lag phase. The results and their statistic analysis revealed no relationship between the beer-spoilage ability and hop-resistance. The degree of resistance to the antibacterial activity of Tetra was strongly affected by the initial pH of culture medium, but there was no correlation between the pH-induced change in the level of resistance and the beer spoilage ability of any L. brevis strain.
ER  -

MATOULKOVÁ, Dagmar; Miroslav NĚMEC a Karel SIGLER. Effect of tetrahydroiso-alpha-acids on the growth of beer-spoiling and –nonspoiling bacteria. \textit{Kvasný Průmysl}. Praha, 2010, č.~10, s.~396-403.

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