Metallothionein – zinc – prostate cancer: pathogenesis and
diagnostic use

D 2010

Metallothionein – zinc – prostate cancer: pathogenesis and diagnostic use

GUMULEC, Jaromír, Natalia Vladimirovna CERNEI, Ondřej ZÍTKA, Michal MASAŘÍK, Petr BABULA et. al.

Základní údaje

Originální název

Metallothionein – zinc – prostate cancer: pathogenesis and diagnostic use

Název česky

Metalothionein – zinek – karcinom prostaty: patogeneze a diagnostické využítí

Autoři

GUMULEC, Jaromír, Natalia Vladimirovna CERNEI, Ondřej ZÍTKA, Michal MASAŘÍK, Petr BABULA, Vojtěch ADAM a René KIZEK

Vydání

Brno, MendelNet 2010, od s. 977-983, 7 s. 2010

Nakladatel

Mendelova Univerzita

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Organizační jednotka

Lékařská fakulta

ISBN

978-80-7375-453-2

Klíčová slova česky

metalothionein;zinek;karcinom prostaty;paramagnetické částice;PC-3;PNT1A;nádorový marker;

Klíčová slova anglicky

metallothionein;zinc;prostate cancer;magnetic particles;PC-3;PNT1A;tumor marker

Změněno: 21. 1. 2011 11:19, prof. RNDr. Michal Masařík, Ph.D.

Anotace

V originále

Prostate cancer (PCa) is one of the most frequent cancer and one of the most frequent cancer-related cause of death among men. Therefore, early diagnosis, differentiation between risky and relative benign forms and understanding of pathogenesis of disease for further therapeutic approaches is highly desirable. Healthy prostate is unique in zinc accumulation. Zinc is (mostly) buffered by cysteine-rich low molecular protein metallothionein (MT). In contrast, PCa has altered zinc metabolism and elevated MT. In PCa patients, MT is elevated even in serum and can therefore be used as potential tumor marker due to high specifity to PCa. This could be very desirable because of inaccuracies of current prostatic specific antigen (PSA) screening. The aims of this study is (1) to analyze MT-zinc relation on cell lines: to determine zinc and MT levels in cell lines PC-3 (cancer) and PNT1A (control), (2) to find relations between MT and PSA, (3) to describe potential effects of MT and/or zinc on prostate cancer pathogenesis, (4) to determine serum MT level,(5) to find relations between MT level and patient’s disease grading. We used (1) optimized fully automated immunochemical methods for detection of serum PSA in serum, (2) protein separation with paramagnetic microparticles modified with antibody against PSA and MT, (3) PAGE gel silver and coomassie staining and colorimetric detection. We found (1) statistically significant (p=0,001) MT elevation in PCa lines and in PCa serum, (2) significant PSA elevation in cell lines, (3) strong correlation between intracel. zinc and MT, (4) no correlation between disease grading/patient’s history, PSA level and MT level. We found MT/zinc play a role in PCa pathogenesis, further understanding may have therapeutic implications. By our findings, MT is a good candidate for new marker for PCa screening, developing of automated diagnostic methods is highly desirable.

Návaznosti

GP301/09/P436, projekt VaV

Název: Analýza metalothioneinu u karcinomu prostaty na úrovni DNA, RNA a proteinu.

Investor: Grantová agentura ČR, Analýza metalothioneinu u karcinomu prostaty na úrovni DNA, RNA a proteinu

Citovat

GUMULEC, Jaromír, Natalia Vladimirovna CERNEI, Ondřej ZÍTKA, Michal MASAŘÍK, Petr BABULA, Vojtěch ADAM a René KIZEK. Metallothionein – zinc – prostate cancer: pathogenesis and diagnostic use. In Ryant Pavel a kol. MendelNet 2010. Brno: Mendelova Univerzita, 2010, s. 977-983. ISBN 978-80-7375-453-2.

@inproceedings{909330,
   author = {Gumulec, Jaromír and Cernei, Natalia Vladimirovna and Zítka, Ondřej and Masařík, Michal and Babula, Petr and Adam, Vojtěch and Kizek, René},
   address = {Brno},
   booktitle = {MendelNet 2010},
   editor = {Ryant Pavel a kol.},
   keywords = {metallothionein;zinc;prostate cancer;magnetic particles;PC-3;PNT1A;tumor marker},
   language = {eng},
   location = {Brno},
   isbn = {978-80-7375-453-2},
   pages = {977-983},
   publisher = {Mendelova Univerzita},
   title = {Metallothionein – zinc – prostate cancer: pathogenesis and diagnostic use},
   url = {http://www.af.mendelu.cz/mendelnet},
   year = {2010}
}

TY  - JOUR
ID  - 909330
AU  - Gumulec, Jaromír - Cernei, Natalia Vladimirovna - Zítka, Ondřej - Masařík, Michal - Babula, Petr - Adam, Vojtěch - Kizek, René
PY  - 2010
TI  - Metallothionein – zinc – prostate cancer: pathogenesis and diagnostic use
PB  - Mendelova Univerzita
CY  - Brno
SN  - 9788073754532
KW  - metallothionein;zinc;prostate cancer;magnetic particles;PC-3;PNT1A;tumor marker
UR  - http://www.af.mendelu.cz/mendelnet
N2  - Prostate cancer (PCa) is one of the most frequent cancer and one of the most frequent cancer-related cause of death among men. Therefore, early diagnosis, differentiation between risky and relative benign forms and understanding of pathogenesis of disease for further therapeutic approaches is highly desirable. Healthy prostate is unique in zinc accumulation. Zinc is (mostly) buffered by cysteine-rich low molecular protein metallothionein (MT). In contrast, PCa has altered zinc metabolism and elevated MT. In PCa patients, MT is elevated even in serum and can therefore be used as potential tumor marker due to high specifity to PCa. This could be very desirable because of inaccuracies of current prostatic specific antigen (PSA) screening. The aims of this study is (1) to analyze MT-zinc relation on cell lines: to determine zinc and MT levels in cell lines PC-3 (cancer) and PNT1A (control), (2) to find relations between MT and PSA, (3) to describe potential effects of MT and/or zinc on prostate cancer pathogenesis, (4) to determine serum MT level,(5) to find relations between MT level and patient’s disease grading. We used (1) optimized fully automated immunochemical methods for detection of serum PSA in serum, (2) protein separation with paramagnetic microparticles modified with antibody against PSA and MT, (3) PAGE gel silver and coomassie staining and colorimetric detection. We found (1) statistically significant (p=0,001) MT elevation in PCa lines and in PCa serum, (2) significant PSA elevation in cell lines, (3) strong correlation between intracel. zinc and MT, (4) no correlation between disease grading/patient’s history, PSA level and MT level. We found MT/zinc play a role in PCa pathogenesis, further understanding may have therapeutic implications. By our findings, MT is a good candidate for new marker for PCa screening, developing of automated diagnostic methods is highly desirable.
ER  -

GUMULEC, Jaromír, Natalia Vladimirovna CERNEI, Ondřej ZÍTKA, Michal MASAŘÍK, Petr BABULA, Vojtěch ADAM a René KIZEK. Metallothionein – zinc – prostate cancer: pathogenesis and diagnostic use. In Ryant Pavel a kol. \textit{MendelNet 2010}. Brno: Mendelova Univerzita, 2010, s.~977-983. ISBN~978-80-7375-453-2.

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