VAŠÍČEK, Ondřej, Ivana PAPEŽÍKOVÁ a Pavel HYRŠL. Fluorimetric determination of hydrogen peroxide production by the haemocytes of the wax moth Galleria mellonella L. (Lepidoptera: Pyralidae). European Journal of Entomology. roč. 108, č. 108, s. 481-485, 6 s. ISSN 1210-5759. 2010.
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Základní údaje
Originální název Fluorimetric determination of hydrogen peroxide production by the haemocytes of the wax moth Galleria mellonella L. (Lepidoptera: Pyralidae)
Název česky Fluorimetric determination of hydrogen peroxide production by the haemocytes of the wax moth Galleria mellonella L. (Lepidoptera: Pyralidae)
Autoři VAŠÍČEK, Ondřej (203 Česká republika), Ivana PAPEŽÍKOVÁ (203 Česká republika) a Pavel HYRŠL (203 Česká republika, garant, domácí).
Vydání European Journal of Entomology, 2010, 1210-5759.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 30105 Physiology
Stát vydavatele Česká republika
Utajení není předmětem státního či obchodního tajemství
Impakt faktor Impact factor: 0.945
Kód RIV RIV/00216224:14310/10:00045625
Organizační jednotka Přírodovědecká fakulta
UT WoS 000291955100020
Klíčová slova česky Galleria mellonella; chemiluminescence; fluorescence; hydrogen peroxide; reactive oxygen species; Amplex Red; haemocytes; phagocytes; leukocytes; phagocytosis
Klíčová slova anglicky Galleria mellonella; chemiluminescence; fluorescence; hydrogen peroxide; reactive oxygen species; Amplex Red; haemocytes; phagocytes; leukocytes; phagocytosis
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnil: doc. RNDr. Pavel Hyršl, Ph.D., učo 9982. Změněno: 20. 5. 2011 12:21.
Anotace
The aim of this work was to investigate whether haemocytes of Galleria mellonella (Lepidoptera: Pyralidae) larvae can produce reactive oxygen species (ROS) in comparison to human blood phagocytes. The production of ROS was measured first with the use of luminol - enhanced chemiluminescence of unstimulated and stimulated (four activators with different mode of action) haemolymph or isolated haemocytes. However, spontaneous as well as the activated ROS generation remained at the background level. In subsequent experiments an ultrasensitive fluorescence method using Amplex Red reagent to detect hydrogen peroxide (H2O2) was tested. After optimization, Amplex Red was successfully used for determination of H2O2 production in both unstimulated and stimulated haemocytes. To determine the role of pH and ions during measurement, several diluent solutions were tested. We have shown that Ca2+ and Mg2+ ions are less important for the reaction in insects as compared to mammalian cells.
Anotace česky
The aim of this work was to investigate whether haemocytes of Galleria mellonella (Lepidoptera: Pyralidae) larvae can produce reactive oxygen species (ROS) in comparison to human blood phagocytes. The production of ROS was measured first with the use of luminol - enhanced chemiluminescence of unstimulated and stimulated (four activators with different mode of action) haemolymph or isolated haemocytes. However, spontaneous as well as the activated ROS generation remained at the background level. In subsequent experiments an ultrasensitive fluorescence method using Amplex Red reagent to detect hydrogen peroxide (H2O2) was tested. After optimization, Amplex Red was successfully used for determination of H2O2 production in both unstimulated and stimulated haemocytes. To determine the role of pH and ions during measurement, several diluent solutions were tested. We have shown that Ca2+ and Mg2+ ions are less important for the reaction in insects as compared to mammalian cells.
Návaznosti
GP206/09/P470, projekt VaVNázev: Vliv inhibitorů biosyntézy eikosanoidů na imunitu zavíječe voskového Galleria mellonella
Investor: Grantová agentura ČR, Vliv inhibitorů biosyntézy eikonsanoidů na imunitu zavíječe voskového Galleria mellonella
VytisknoutZobrazeno: 29. 3. 2024 12:32