2010
Fluorimetric determination of hydrogen peroxide production by the haemocytes of the wax moth Galleria mellonella L. (Lepidoptera: Pyralidae)
VAŠÍČEK, Ondřej; Ivana PAPEŽÍKOVÁ a Pavel HYRŠLZákladní údaje
Originální název
Fluorimetric determination of hydrogen peroxide production by the haemocytes of the wax moth Galleria mellonella L. (Lepidoptera: Pyralidae)
Název česky
Fluorimetric determination of hydrogen peroxide production by the haemocytes of the wax moth Galleria mellonella L. (Lepidoptera: Pyralidae)
Autoři
VAŠÍČEK, Ondřej; Ivana PAPEŽÍKOVÁ a Pavel HYRŠL
Vydání
European Journal of Entomology, 2010, 1210-5759
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
30105 Physiology
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 0.945
Označené pro přenos do RIV
Ano
Kód RIV
RIV/00216224:14310/10:00045625
Organizační jednotka
Přírodovědecká fakulta
UT WoS
Klíčová slova česky
Galleria mellonella; chemiluminescence; fluorescence; hydrogen peroxide; reactive oxygen species; Amplex Red; haemocytes; phagocytes; leukocytes; phagocytosis
Klíčová slova anglicky
Galleria mellonella; chemiluminescence; fluorescence; hydrogen peroxide; reactive oxygen species; Amplex Red; haemocytes; phagocytes; leukocytes; phagocytosis
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 20. 5. 2011 12:21, doc. RNDr. Pavel Hyršl, Ph.D.
V originále
The aim of this work was to investigate whether haemocytes of Galleria mellonella (Lepidoptera: Pyralidae) larvae can produce reactive oxygen species (ROS) in comparison to human blood phagocytes. The production of ROS was measured first with the use of luminol - enhanced chemiluminescence of unstimulated and stimulated (four activators with different mode of action) haemolymph or isolated haemocytes. However, spontaneous as well as the activated ROS generation remained at the background level. In subsequent experiments an ultrasensitive fluorescence method using Amplex Red reagent to detect hydrogen peroxide (H2O2) was tested. After optimization, Amplex Red was successfully used for determination of H2O2 production in both unstimulated and stimulated haemocytes. To determine the role of pH and ions during measurement, several diluent solutions were tested. We have shown that Ca2+ and Mg2+ ions are less important for the reaction in insects as compared to mammalian cells.
Česky
The aim of this work was to investigate whether haemocytes of Galleria mellonella (Lepidoptera: Pyralidae) larvae can produce reactive oxygen species (ROS) in comparison to human blood phagocytes. The production of ROS was measured first with the use of luminol - enhanced chemiluminescence of unstimulated and stimulated (four activators with different mode of action) haemolymph or isolated haemocytes. However, spontaneous as well as the activated ROS generation remained at the background level. In subsequent experiments an ultrasensitive fluorescence method using Amplex Red reagent to detect hydrogen peroxide (H2O2) was tested. After optimization, Amplex Red was successfully used for determination of H2O2 production in both unstimulated and stimulated haemocytes. To determine the role of pH and ions during measurement, several diluent solutions were tested. We have shown that Ca2+ and Mg2+ ions are less important for the reaction in insects as compared to mammalian cells.
Návaznosti
| GP206/09/P470, projekt VaV |
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