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@inproceedings{911208,
author = {Pekárová, Blanka and Klumpler, Tomáš and Třísková, Olga and Horák, Jakub and Žídek, Lukáš and Marek, Jaromír and Dopitová, Radka and Hejátko, Jan and Janda, Lubomír},
address = {Tarragona, Spain},
keywords = {multistep phosphorelay, crystal structure, NMR analysis, CKI1},
language = {eng},
location = {Tarragona, Spain},
pages = {85-86},
publisher = {IPGSA conference 2010},
title = {Dynamic structure and binding specifity of the receiver domain of sensor histidine kinase CKI1},
url = {http://events.emagister.co.uk/conferences/20th_ipgsa_conference__international_plant_growth_substances_association/14212},
year = {2010}
}
TY - JOUR
ID - 911208
AU - Pekárová, Blanka - Klumpler, Tomáš - Třísková, Olga - Horák, Jakub - Žídek, Lukáš - Marek, Jaromír - Dopitová, Radka - Hejátko, Jan - Janda, Lubomír
PY - 2010
TI - Dynamic structure and binding specifity of the receiver domain of sensor histidine kinase CKI1
PB - IPGSA conference 2010
CY - Tarragona, Spain
KW - multistep phosphorelay, crystal structure, NMR analysis, CKI1
UR - http://events.emagister.co.uk/conferences/20th_ipgsa_conference__international_plant_growth_substances_association/14212
N2 - In the Arabidopsis two-component signaling, the signal is transfered from sensor histidine kinase via histidine-containing phosphotransfer proteins (AHP1-5) to nuclear response regulators. Here we show the receiver domain of histidine kinase CYTOKININ-INDEPENDENT1 (CKI1RD) interacts in vivo and in vitro with AHP2, 3 and 5 with different affinities. To unravel the molecular determinants of observed specificity, we determined crystal structure of free CKI1RD and CKI1RD in complex with magnesium ions. Dynamics of CKI1RD solution structure has been studied in details by NMR in absence or presence of magnesium ions and beryllium fluoride.
ER -
PEKÁROVÁ, Blanka, Tomáš KLUMPLER, Olga TŘÍSKOVÁ, Jakub HORÁK, Lukáš ŽÍDEK, Jaromír MAREK, Radka DOPITOVÁ, Jan HEJÁTKO a Lubomír JANDA. \textit{Dynamic structure and binding specifity of the receiver domain of sensor histidine kinase CKI1}. Tarragona, Spain: IPGSA conference 2010, 2010, s.~85-86.
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