Genome analysis of Treponema pallidum subsp. pallidum and
subsp. pertenue strains by whole genome fingerprinting: most of
the genetic differences are localized in six genomic regions.

J 2010

Genome analysis of Treponema pallidum subsp. pallidum and subsp. pertenue strains by whole genome fingerprinting: most of the genetic differences are localized in six genomic regions.

MIKALOVÁ, Lenka; Michal STROUHAL; Darina ČEJKOVÁ; Marie ZOBANÍKOVÁ; Petra POSPÍŠILOVÁ et al.

Základní údaje

Originální název

Genome analysis of Treponema pallidum subsp. pallidum and subsp. pertenue strains by whole genome fingerprinting: most of the genetic differences are localized in six genomic regions.

Autoři

MIKALOVÁ, Lenka ; Michal STROUHAL; Darina ČEJKOVÁ; Marie ZOBANÍKOVÁ; Petra POSPÍŠILOVÁ; S.J. NORRIS; E. SODERGREN; G.M. WEINSTOCK a David ŠMAJS

Vydání

PloS ONE, San Francisco, Public Library of Science, 2010, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.411

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/10:00040816

Organizační jednotka

Lékařská fakulta

UT WoS

000285793200041

Klíčová slova anglicky

Treponema pallidum subsp. pallidum; Treponema pallidum subsp. pertenue; syphilis; yaws;

Změněno: 4. 1. 2011 11:05, Mgr. Marie Zobaníková, Ph.D.

Anotace

V originále

The genomes of eight treponemes including T. p. pallidum strains (Nichols, SS14, DAL1 and Mexico A), T. p. pertenue strains (Samoa D, CDC2 and Gauthier), and the FribourgBlanc isolate, were amplified in 133 overlapping amplicons, and the restriction patterns of these fragments were compared. The approximate genome sequence identity investigated based on this whole genome fingerprinting (WGF) analysis ranged from 99.57 to 99.98% in the homologous genome regions. Restriction target site analysis, detecting the presence of 1773 individual restriction sites found in the reference Nichols genome, revealed a high genome structure similarity of all strains. Most of the genetic differences between T. p. pallidum and T. p. pertenue strains were accumulated in six genomic regions. These genome differences likely contribute to the observed differences in pathogenicity between T. p. pallidum and T. p. pertenue strains and could be used for the molecular detection.

Návaznosti

GA310/07/0321, projekt VaV

Název: Komparativní genomové sekvencování patogenních treponem a transkriptomová analýza T. pallidum ssp. pallidum

Investor: Grantová agentura ČR, Komparativní genomové sekvencování patogenních treponem a transkriptomová analýza T. pallidum ssp. pallidum

MSM0021622415, záměr

Název: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární podstata buněčných a tkáňových regulací

NR8967, projekt VaV

Název: Molekulární typizace kmenů a izolátů Treponema pallidum subsp. pallidum: základ pro epidemiologickou a klinickou identifikaci jednotlivých kmenů

Investor: Ministerstvo zdravotnictví ČR, Molekulární typizace kmenů a izolátů Treponema pallidum subsp. Pallidum: základ pro epidemiologickou a klinickou identifikaci jednotlivých kmenů

Citovat

MIKALOVÁ, Lenka; Michal STROUHAL; Darina ČEJKOVÁ; Marie ZOBANÍKOVÁ; Petra POSPÍŠILOVÁ; S.J. NORRIS; E. SODERGREN; G.M. WEINSTOCK a David ŠMAJS. Genome analysis of Treponema pallidum subsp. pallidum and subsp. pertenue strains by whole genome fingerprinting: most of the genetic differences are localized in six genomic regions. PloS ONE. San Francisco: Public Library of Science, 2010, roč. 5, č. 12, s. e15713, 13 s. ISSN 1932-6203.

@article{913379,
   author = {Mikalová, Lenka and Strouhal, Michal and Čejková, Darina and Zobaníková, Marie and Pospíšilová, Petra and Norris, S.J. and Sodergren, E. and Weinstock, G.M. and Šmajs, David},
   article_location = {San Francisco},
   article_number = {12},
   keywords = {Treponema pallidum subsp. pallidum; Treponema pallidum subsp. pertenue; syphilis; yaws;},
   language = {eng},
   issn = {1932-6203},
   journal = {PloS ONE},
   title = {Genome analysis of Treponema pallidum subsp. pallidum and subsp. pertenue strains by whole genome fingerprinting: most of the genetic differences are localized in six genomic regions.},
   url = {http://www.plosone.org/article/info:doi/10.1371/journal.pone.0015713},
   volume = {5},
   year = {2010}
}

TY  - JOUR
ID  - 913379
AU  - Mikalová, Lenka - Strouhal, Michal - Čejková, Darina - Zobaníková, Marie - Pospíšilová, Petra - Norris, S.J. - Sodergren, E. - Weinstock, G.M. - Šmajs, David
PY  - 2010
TI  - Genome analysis of Treponema pallidum subsp. pallidum and subsp. pertenue strains by whole genome fingerprinting: most of the genetic differences are localized in six genomic regions.
JF  - PloS ONE
VL  - 5
IS  - 12
SP  - e15713
EP  - e15713
PB  - Public Library of Science
SN  - 19326203
KW  - Treponema pallidum subsp. pallidum
KW  - Treponema pallidum subsp. pertenue
KW  - syphilis
KW  - yaws;
UR  - http://www.plosone.org/article/info:doi/10.1371/journal.pone.0015713
N2  - The genomes of eight treponemes including T. p. pallidum strains (Nichols, SS14, DAL1 and Mexico A), T. p. pertenue strains (Samoa D, CDC2 and Gauthier), and the FribourgBlanc isolate, were amplified in 133 overlapping amplicons, and the restriction patterns of these fragments were compared. The approximate genome sequence identity investigated based on this whole genome fingerprinting (WGF) analysis ranged from 99.57 to 99.98% in the homologous genome regions. Restriction target site analysis, detecting the presence of 1773 individual restriction sites found in the reference Nichols genome, revealed a high genome structure similarity of all strains. Most of the genetic differences between T. p. pallidum and T. p. pertenue strains were accumulated in six genomic regions. These genome differences likely contribute to the observed differences in pathogenicity between T. p. pallidum and T. p. pertenue strains and could be used for the molecular detection.
ER  -

MIKALOVÁ, Lenka; Michal STROUHAL; Darina ČEJKOVÁ; Marie ZOBANÍKOVÁ; Petra POSPÍŠILOVÁ; S.J. NORRIS; E. SODERGREN; G.M. WEINSTOCK a David ŠMAJS. Genome analysis of Treponema pallidum subsp. pallidum and subsp. pertenue strains by whole genome fingerprinting: most of the genetic differences are localized in six genomic regions. \textit{PloS ONE}. San Francisco: Public Library of Science, 2010, roč.~5, č.~12, s.~e15713, 13 s. ISSN~1932-6203.

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