2010
Isolation of granulocytes: Which transcriptome do we analyze neutrophils or eosinophils?
STEJSKAL, Stanislav, Irena KRONTORÁD KOUTNÁ a Zdeněk RUČKAZákladní údaje
Originální název
Isolation of granulocytes: Which transcriptome do we analyze neutrophils or eosinophils?
Autoři
STEJSKAL, Stanislav (203 Česká republika, domácí), Irena KRONTORÁD KOUTNÁ (203 Česká republika, garant, domácí) a Zdeněk RUČKA (203 Česká republika, domácí)
Vydání
FOLIA BIOLOGICA, 2010, 0015-5500
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
30102 Immunology
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 0.729
Kód RIV
RIV/00216224:14330/10:00067250
Organizační jednotka
Fakulta informatiky
UT WoS
000286039500003
Klíčová slova anglicky
total RNA; gradient centrifugation; cell isolation; magnetic separation; gene expression; expression activity; neutrophils; eosinophils; granulocytes
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 30. 7. 2013 12:13, Ing. Mgr. Věra Pospíšilíková
Anotace
V originále
Isolation of granulocyte cells from blood is necessary for accurate studying of changes in their expression. After gradient centrifugation, we obtain relatively pure granulocyte populations with different ratios of neutrophils and eosinophils. Unfortunately, in many studies in this field the expression results are not set according to the real variability of the granulocyte population. In many cases, the granulocyte population is marked simply as neutrophils and the residual population of eosinophils is not considered. Based on our recent study where we tracked the general transcription factor RNA polymerase II (RNAP II), we hypothesized that eosinophils are more transcriptionally active cells than neutrophils. We decided to test our hypothesis on isolated cells because its implications could change our view on many past expression analyses performed on granulocytes. In our experiments, we isolated neutrophils and eosinophils and measured their total RNA production. According to our results, eosinophils produce much more RNA than neutrophils. Therefore, relatively low numbers of highly active eosinophils can markedly affect the whole pool of granulocytic RNA. We want to emphasize that either a detailed description of the cell population or the use of a pure neutrophil population is necessary for the correct interpretation of neutrophil expression analysis results.
Návaznosti
| MSM0021622419, záměr |
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| MSM0021622430, záměr |
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| 2B06052, projekt VaV |
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