HIGH GLUCOSE INCREASES SUSCEPTIBILITY TO
OXIDATIVE-STRESS-INDUCED APOPTOSIS AND DNA DAMAGE IN K-562
CELLS

J 2010

HIGH GLUCOSE INCREASES SUSCEPTIBILITY TO OXIDATIVE-STRESS-INDUCED APOPTOSIS AND DNA DAMAGE IN K-562 CELLS

HRUDA, Jan; Vladimír ŠRÁMEK a Xavier LEVERVE

Základní údaje

Originální název

HIGH GLUCOSE INCREASES SUSCEPTIBILITY TO OXIDATIVE-STRESS-INDUCED APOPTOSIS AND DNA DAMAGE IN K-562 CELLS

Autoři

HRUDA, Jan; Vladimír ŠRÁMEK a Xavier LEVERVE

Vydání

Biomedical Papers, Olomouc, Palacky University, 2010, 1213-8118

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 0.716

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/10:00051723

Organizační jednotka

Lékařská fakulta

UT WoS

000285643800007

Klíčová slova anglicky

K-562 cells; Oxidative stress; Apoptosis; Energy substrates; Hyperglycemia; DNA damage

Příznaky

Mezinárodní význam

Změněno: 26. 1. 2012 11:47, Mgr. Michal Petr

Anotace

V originále

The study was carried out to evaluate the effect of several substrates on oxidative stress induced apoptosis and in K-562 cells. Methods. Glucose at 5, 11 and 30 mM concentrations was tested, as well as 5 mM glutamine and 5 mM fructose. The cells were exposed to tert-butylhydroperoxide (tBH) and apoptotic cells were evaluated by flow cytometry with FITC-Annexin V and propidium iodide. The effect of glucose concentration on DNA damage was evaluated using hydrogen peroxide and electrophoretic “DNA comets” assay at 5 mM and 30 mM glucose concentrations. Results. The exposure of cells to tBH resulted in increased number of apoptotic cells, and this effect was prevented by administration of an antioxidant – N-Acetyl cysteine. Rising concentrations of glucose added to the toxic effect of tBH; we also observed some toxic effect of fructose and no effect of glutamine. We found higher susceptibility to hydrogen peroxide induced DNA damage with 30 mM glucose concentration. Conclusion. Hyperglycemia increases the cell’s susceptibility to oxidative stress and it also amplifies oxidative DNA damage. Glutamine – when used as a sole energetic substrate – showed no protective effect against oxidative stress.

Citovat

HRUDA, Jan; Vladimír ŠRÁMEK a Xavier LEVERVE. HIGH GLUCOSE INCREASES SUSCEPTIBILITY TO OXIDATIVE-STRESS-INDUCED APOPTOSIS AND DNA DAMAGE IN K-562 CELLS. Biomedical Papers. Olomouc: Palacky University, 2010, roč. 154, č. 4, s. 315-320. ISSN 1213-8118.

@article{920781,
   author = {Hruda, Jan and Šrámek, Vladimír and Leverve, Xavier},
   article_location = {Olomouc},
   article_number = {4},
   keywords = {K-562 cells; Oxidative stress; Apoptosis; Energy substrates; Hyperglycemia; DNA damage},
   language = {eng},
   issn = {1213-8118},
   journal = {Biomedical Papers},
   title = {HIGH GLUCOSE INCREASES SUSCEPTIBILITY TO OXIDATIVE-STRESS-INDUCED APOPTOSIS AND DNA DAMAGE IN K-562 CELLS},
   volume = {154},
   year = {2010}
}

TY  - JOUR
ID  - 920781
AU  - Hruda, Jan - Šrámek, Vladimír - Leverve, Xavier
PY  - 2010
TI  - HIGH GLUCOSE INCREASES SUSCEPTIBILITY TO OXIDATIVE-STRESS-INDUCED APOPTOSIS AND DNA DAMAGE IN K-562 CELLS
JF  - Biomedical Papers
VL  - 154
IS  - 4
SP  - 315-320
EP  - 315-320
PB  - Palacky University
SN  - 12138118
KW  - K-562 cells
KW  - Oxidative stress
KW  - Apoptosis
KW  - Energy substrates
KW  - Hyperglycemia
KW  - DNA damage
N2  - The study was carried out to evaluate the effect of several substrates on oxidative stress induced apoptosis and in K-562 cells. Methods. Glucose at 5, 11 and 30 mM concentrations was tested, as well as 5 mM glutamine and 5 mM fructose. The cells were exposed to tert-butylhydroperoxide (tBH) and apoptotic cells were evaluated by flow cytometry with FITC-Annexin V and propidium iodide. The effect of glucose concentration on DNA damage was evaluated using hydrogen peroxide and electrophoretic “DNA comets” assay at 5 mM and 30 mM glucose concentrations. Results. The exposure of cells to tBH resulted in increased number of apoptotic cells, and this effect was prevented by administration of an antioxidant – N-Acetyl cysteine. Rising concentrations of glucose added to the toxic effect of tBH; we also observed some toxic effect of fructose and no effect of glutamine. We found higher susceptibility to hydrogen peroxide induced DNA damage with 30 mM glucose concentration. Conclusion. Hyperglycemia increases the cell’s susceptibility to oxidative stress and it also amplifies oxidative DNA damage. Glutamine – when used as a sole energetic substrate – showed no protective effect against oxidative stress.
ER  -

HRUDA, Jan; Vladimír ŠRÁMEK a Xavier LEVERVE. HIGH GLUCOSE INCREASES SUSCEPTIBILITY TO OXIDATIVE-STRESS-INDUCED APOPTOSIS AND DNA DAMAGE IN K-562 CELLS. \textit{Biomedical Papers}. Olomouc: Palacky University, 2010, roč.~154, č.~4, s.~315-320. ISSN~1213-8118.

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