Characterization of Lymphocyte Subsets in Patients with common
variable immunodeficiency reveals subsets of naive human B
cells marked by CD24 expression

J 2010

Characterization of Lymphocyte Subsets in Patients with common variable immunodeficiency reveals subsets of naive human B cells marked by CD24 expression

VLKOVÁ, Marcela; E. FROŇKOVÁ; V. KANDEROVÁ; A. JANDA; Š. RŮŽIČKOVÁ et. al.

Základní údaje

Originální název

Characterization of Lymphocyte Subsets in Patients with common variable immunodeficiency reveals subsets of naive human B cells marked by CD24 expression

Název česky

Charakterizace lymfocytárních subpopulací u pacientů s CVID rozděluje subpopulace naivních lidských B lymfkocytů na základě exprese znaku CD24

Autoři

VLKOVÁ, Marcela (203 Česká republika, garant, domácí); E. FROŇKOVÁ (203 Česká republika); V. KANDEROVÁ (203 Česká republika); A. JANDA (203 Česká republika); Š. RŮŽIČKOVÁ (203 Česká republika); Jiří LITZMAN (203 Česká republika, domácí); A. ŠEDIVÁ (203 Česká republika) a T. KALINA (203 Česká republika)

Vydání

Journal of immunology, Bethesda, American association of immunologists, 2010, 0022-1767

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30102 Immunology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 5.745

Kód RIV

RIV/00216224:14110/10:00058956

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.4049/jimmunol.0903876

UT WoS

000284311500007

Klíčová slova česky

B lymfocyty; průtokový cytometrie; CVID

Klíčová slova anglicky

B cells; flow cytometry; CVID

Příznaky

Mezinárodní význam

Změněno: 4. 10. 2012 14:06, Mgr. Michal Petr

Anotace

ORIG CZ

V originále

Increased proportions of naive B cell subset and B cells defined as CD27negCD21negCD38neg are frequently found in patients with common variable immunodeficiency (CVID) syndrome. Current methods of polychromatic flow cytometry and PCR-based detection of k deletion excision circles allow for fine definitions and replication history mapping of infrequent B cell subsets. We have analyzed B cells from 48 patients with CVID and 49 healthy controls to examine phenotype, frequency, and proliferation history of naive B cell subsets. Consistent with previous studies, we have described two groups of patients with normal (CVID 21norm) or increased (CVID 21lo) proportions of CD27negCD21negCD38neg B cells. Upon further analyses, we found two discrete subpopulations of this subset based on the expression of CD24. The B cell subsets showed a markedly increased proliferation in CVID 21lo patients as compared with healthy controls, suggesting developmental arrest rather than increased bone marrow output. Furthermore, when we analyzed CD21pos naive B cells, we found two different subpopulations based on IgM and CD24 expression. They correspond to follicular (FO) I and FO II cells previously described in mice. FO I subset is significantly underrepresented in CVID 21lo patients. A comparison of the replication history of naive B cell subsets in CVID patients and healthy controls implies refined naive B cell developmental scheme, in which human transitional B cells develop into FO II and FO I. We propose that the CD27negCD21negCD38neg B cells increased in some of the CVID patients originate from the two FO subsets after loss of CD21 expression.

Česky

Při studiu B lymfocytárních subpopulací u pacientů s CVID byly nalezeny nové subpopulace naivních B lymfocytů na základě exprese znaků CD24 a povrchového IgM. Tyto subpopulace byly poté nalezeny i u všech kontrolních osob.

Citovat

VLKOVÁ, Marcela; E. FROŇKOVÁ; V. KANDEROVÁ; A. JANDA; Š. RŮŽIČKOVÁ; Jiří LITZMAN; A. ŠEDIVÁ a T. KALINA. Characterization of Lymphocyte Subsets in Patients with common variable immunodeficiency reveals subsets of naive human B cells marked by CD24 expression. Journal of immunology. Bethesda: American association of immunologists, 2010, roč. 185, č. 11, s. 6431-6438. ISSN 0022-1767. Dostupné z: https://doi.org/10.4049/jimmunol.0903876.

@article{920883,
   author = {Vlková, Marcela and Froňková, E. and Kanderová, V. and Janda, A. and Růžičková, Š. and Litzman, Jiří and Šedivá, A. and Kalina, T.},
   article_location = {Bethesda},
   article_number = {11},
   doi = {https://doi.org/10.4049/jimmunol.0903876},
   keywords = {B cells; flow cytometry; CVID},
   language = {eng},
   issn = {0022-1767},
   journal = {Journal of immunology},
   title = {Characterization of Lymphocyte Subsets in Patients with common variable immunodeficiency reveals subsets of naive human B cells marked by CD24 expression},
   volume = {185},
   year = {2010}
}

TY  - JOUR
ID  - 920883
AU  - Vlková, Marcela - Froňková, E. - Kanderová, V. - Janda, A. - Růžičková, Š. - Litzman, Jiří - Šedivá, A. - Kalina, T.
PY  - 2010
TI  - Characterization of Lymphocyte Subsets in Patients with common variable immunodeficiency reveals subsets of naive human B cells marked by CD24 expression
JF  - Journal of immunology
VL  - 185
IS  - 11
SP  - 6431-6438
EP  - 6431-6438
PB  - American association of immunologists
SN  - 00221767
KW  - B cells
KW  - flow cytometry
KW  - CVID
N2  - Increased proportions of naive B cell subset and B cells defined as CD27negCD21negCD38neg are frequently found in patients with common variable immunodeficiency (CVID) syndrome. Current methods of polychromatic flow cytometry and PCR-based detection of k deletion excision circles allow for fine definitions and replication history mapping of infrequent B cell subsets. We have analyzed B cells from 48 patients with CVID and 49 healthy controls to examine phenotype, frequency, and proliferation history of naive B cell subsets. Consistent with previous studies, we have described two groups of patients with normal (CVID 21norm) or increased (CVID 21lo) proportions of CD27negCD21negCD38neg B cells. Upon further analyses, we found two discrete subpopulations of this subset based on the expression of CD24. The B cell subsets showed a markedly increased proliferation in CVID 21lo patients as compared with healthy controls, suggesting developmental arrest rather than increased bone marrow output. Furthermore, when we analyzed CD21pos naive B cells, we found two different subpopulations based on IgM and CD24 expression. They correspond to follicular (FO) I and FO II cells previously described in mice. FO I subset is significantly underrepresented in CVID 21lo patients. A comparison of the replication history of naive B cell subsets in CVID patients and healthy controls implies refined naive B cell developmental scheme, in which human transitional B cells develop into FO II and FO I. We propose that the CD27negCD21negCD38neg B cells increased in some of the CVID patients originate from the two FO subsets after loss of CD21 expression.
ER  -

VLKOVÁ, Marcela; E. FROŇKOVÁ; V. KANDEROVÁ; A. JANDA; Š. RŮŽIČKOVÁ; Jiří LITZMAN; A. ŠEDIVÁ a T. KALINA. Characterization of Lymphocyte Subsets in Patients with common variable immunodeficiency reveals subsets of naive human B cells marked by CD24 expression. \textit{Journal of immunology}. Bethesda: American association of immunologists, 2010, roč.~185, č.~11, s.~6431-6438. ISSN~0022-1767. Dostupné z: https://doi.org/10.4049/jimmunol.0903876.

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