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@inbook{922230,
author = {Martin, Harry and Burgess, Elisabeth and Masařík, Michal and Kramer, Karl and Beklová, Miroslava and Adam, Vojtěch and Kizek, René},
address = {New York},
booktitle = {Genetic Engineering, Biofertilisation, Soil Quality and Organic Farming},
edition = {1st Edition},
keywords = {Transgenic plants; avidinbiotin technology; agriculture; electrochemical method},
language = {eng},
location = {New York},
isbn = {978-90-481-8740-9},
pages = {1-22},
publisher = {Springer},
title = {Avidin and plant biotechnology to control pests},
year = {2010}
}
TY - CHAP
ID - 922230
AU - Martin, Harry - Burgess, Elisabeth - Masařík, Michal - Kramer, Karl - Beklová, Miroslava - Adam, Vojtěch - Kizek, René
PY - 2010
TI - Avidin and plant biotechnology to control pests
VL - Sustainable Agriculture Reviews Volume 4
PB - Springer
CY - New York
SN - 9789048187409
KW - Transgenic plants
KW - avidinbiotin technology
KW - agriculture
KW - electrochemical method
N2 - In this review, we discuss the application of transgenic avidin, a protein naturally occurring in eggwhite, in the protection of rice, maize, potato and apple leaf from insect pests. Avidin binds the vitamin, biotin with extraordinary affinity (10minus15 M). Biotin is a watersoluble vitamin that is required for normal cellular metabolism and growth. The presence of avidin in the diet of insect pests is lethal since biotin is unavailable to them. The use of streptavidin, a bacterial homologue of avidin, is also described. We discuss the subcellular targeting of avidin expression in plants to avoid toxicity to the plant host and we describe the qualities of avidin which make it suitable for crop protection during cultivation and storage. Avidin is stable under normal conditions of crop storage but biodegradable and destroyed by cooking. These combined qualities make it an excellent choice for the protection of crops from insects. Finally, we discuss the modification of the avidin gene to allow expression in plants, the methods for transfection of the gene into plants, and the approaches used to quantify gene expression and avidin function in plant tissues. These methods include: polymerase chain reaction; enzymelinked immmunosorbent assay; polyacrylamide gelelectrophoresis; fluorescence polarisation (FP); capillary electrophoresis; tissueprinting; square-wave voltammetry (SWV) and the measurement of larvae morbidity and mortality.
ER -
MARTIN, Harry, Elisabeth BURGESS, Michal MASAŘÍK, Karl KRAMER, Miroslava BEKLOVÁ, Vojtěch ADAM a René KIZEK. Avidin and plant biotechnology to control pests. In \textit{Genetic Engineering, Biofertilisation, Soil Quality and Organic Farming}. 1st Edition. New York: Springer, 2010, s.~1-22. Sustainable Agriculture Reviews Volume 4. ISBN~978-90-481-8740-9.
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