Determination of free cisplatin in medium by differential pulse
polarography after ultrasound and cisplatin treatment of a
cancer cell culture

J 2011

Determination of free cisplatin in medium by differential pulse polarography after ultrasound and cisplatin treatment of a cancer cell culture

BERNARD, Vladan; Lukáš FOJT; Jiřina ŠKORPÍKOVÁ a Vojtěch MORNSTEIN

Základní údaje

Originální název

Determination of free cisplatin in medium by differential pulse polarography after ultrasound and cisplatin treatment of a cancer cell culture

Název česky

Analýza obsahu volné cisplatiny v buněčném mediu diferenční pulsní polarografií po ovlivění nádorových buněčných linií ultrazvukovým polem a cisplatinou

Autoři

BERNARD, Vladan ; Lukáš FOJT; Jiřina ŠKORPÍKOVÁ a Vojtěch MORNSTEIN

Vydání

Indian Journal of Biochemistry & Biophysics, India, National Institute of Science Communicat, 2011, 0301-1208

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10610 Biophysics

Stát vydavatele

Indie

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.142

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/11:00051935

Organizační jednotka

Lékařská fakulta

UT WoS

000288721600009

Klíčová slova česky

cisplatina; diferenční pulsní polarografie; ovariální nádorové buňky; ultrazvuk

Klíčová slova anglicky

cisplatin; difenecial pulse polarography; ovarina cancer cells; ultrasound

Příznaky

Mezinárodní význam

Změněno: 20. 4. 2012 11:50, Mgr. Michal Petr

Anotace

V originále

The in vitro study was carried out for detection of the cisplatin in free form and in culture medium, depending on various conditions of sonodynamic human ovarian cancer cells A2780 treatment by differential pulse polarography (DPP). For sonodynamic treatment, we used cisplatin alone and combined cisplatin/ultrasound treatments. The ultrasound exposure intensity of 1.0 and 2.0 W cm-2 in far field for incubation periods 1, 24 and 48 h was used. The parameters of DPP measurements were - 1 s drop time, 5 mV s-1 voltage scan rate, 50 mV modulation amplitude and negative scanning direction; platinum wire served as counter electrode and Ag|AgCl|3 M KCl as reference electrode. The results showed the dependence of free platinum quantities in culture medium on incubation time and treatment protocol. Thus, the DPP method is suitable for the detection and quantification of free cisplatin in the culture medium of cell suspension. Ultrasound field can be important factor during cytostatic therapy.

Návaznosti

MUNI/C/0092/2009, interní kód MU

Název: Proliferace a další parametry buněčných kultur po kombinované aplikaci ultrazvuku a vybraných cytostatik

Investor: Masarykova univerzita, Proliferace a další parametry buněčných kultur po kombinované aplikaci ultrazvuku a vybraných cytostatik, Kat. C - Podpora vynikajících disertačních prací v oborech lékařství, zdravotnictví, přírodovědy a informatiky - centralizované prostředky + specifický výzkum

Citovat

BERNARD, Vladan; Lukáš FOJT; Jiřina ŠKORPÍKOVÁ a Vojtěch MORNSTEIN. Determination of free cisplatin in medium by differential pulse polarography after ultrasound and cisplatin treatment of a cancer cell culture. Indian Journal of Biochemistry & Biophysics. India: National Institute of Science Communicat, 2011, roč. 48, č. 1, s. 59-62. ISSN 0301-1208.

@article{928209,
   author = {Bernard, Vladan and Fojt, Lukáš and Škorpíková, Jiřina and Mornstein, Vojtěch},
   article_location = {India},
   article_number = {1},
   keywords = {cisplatin; difenecial pulse polarography; ovarina cancer cells; ultrasound},
   language = {eng},
   issn = {0301-1208},
   journal = {Indian Journal of Biochemistry & Biophysics},
   title = {Determination of free cisplatin in medium by differential pulse polarography after ultrasound and cisplatin treatment of a cancer cell culture},
   volume = {48},
   year = {2011}
}

TY  - JOUR
ID  - 928209
AU  - Bernard, Vladan - Fojt, Lukáš - Škorpíková, Jiřina - Mornstein, Vojtěch
PY  - 2011
TI  - Determination of free cisplatin in medium by differential pulse polarography after ultrasound and cisplatin treatment of a cancer cell culture
JF  - Indian Journal of Biochemistry & Biophysics
VL  - 48
IS  - 1
SP  - 59-62
EP  - 59-62
PB  - National Institute of Science Communicat
SN  - 03011208
KW  - cisplatin
KW  - difenecial pulse polarography
KW  - ovarina cancer cells
KW  - ultrasound
N2  - The in vitro study was carried out for detection of the cisplatin in free form and in culture medium, depending on various conditions of sonodynamic human ovarian cancer cells A2780 treatment by differential pulse polarography (DPP). For sonodynamic treatment, we used cisplatin alone and combined cisplatin/ultrasound treatments. The ultrasound exposure intensity of 1.0 and 2.0 W cm-2 in far field for incubation periods 1, 24 and 48 h was used. The parameters of DPP measurements were - 1 s drop time, 5 mV s-1 voltage scan rate, 50 mV modulation amplitude and negative scanning direction; platinum wire served as counter electrode and Ag|AgCl|3 M KCl as reference electrode. The results showed the dependence of free platinum quantities in culture medium on incubation time and treatment protocol. Thus, the DPP method is suitable for the detection and quantification of free cisplatin in the culture medium of cell suspension. Ultrasound field can be important factor during cytostatic therapy.
ER  -

BERNARD, Vladan; Lukáš FOJT; Jiřina ŠKORPÍKOVÁ a Vojtěch MORNSTEIN. Determination of free cisplatin in medium by differential pulse polarography after ultrasound and cisplatin treatment of a cancer cell culture. \textit{Indian Journal of Biochemistry \&{} Biophysics}. India: National Institute of Science Communicat, 2011, roč.~48, č.~1, s.~59-62. ISSN~0301-1208.

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