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@article{928806,
author = {Čajánek, Lukáš and Adlerz, Linda and Bryja, Vítězslav and Arenas, Ernest},
article_number = {5},
keywords = {WNT pathway; ligand; purification; signaling; AKT/GSK3; phosphorylation; SFRP1; beta-catenin},
language = {eng},
issn = {0730-2312},
journal = {Journal of Cellular Biochemistry},
title = {WNT unrelated activities in commercially available preparations of recombinant WNT3a},
volume = {111},
year = {2010}
}
TY - JOUR
ID - 928806
AU - Čajánek, Lukáš - Adlerz, Linda - Bryja, Vítězslav - Arenas, Ernest
PY - 2010
TI - WNT unrelated activities in commercially available preparations of recombinant WNT3a
JF - Journal of Cellular Biochemistry
VL - 111
IS - 5
SP - 1077-1079
EP - 1077-1079
SN - 07302312
KW - WNT pathway
KW - ligand
KW - purification
KW - signaling
KW - AKT/GSK3
KW - phosphorylation
KW - SFRP1
KW - beta-catenin
N2 - WNT signaling pathways play an important role in both development and disease. By analyzing the signaling capabilities of commercially available WNT3a preparations towards the PI3K/AKT/GSK3 signaling pathway, we discovered unexpected inconsistencies from lot to lot of recombinant WNT3a. We provide evidence that: (1) The ability to trigger AKT/GSK3 signaling varies dramatically between different lots of WNT3a, without any variation in their ability to activate the canonical WNT/beta-catenin signaling. (2) sFRP1, a WNT signaling inhibitor, is unable to interfere with the activation of AKT/GSK3 signaling induced by some of the WNT3a lots. (3) Pharmacological inhibition of AKT/GSK3 phosphorylation by PI3K inhibitors fails to affect the stabilization of beta-catenin, the central effector of the canonical WNT/beta-catenin signaling pathway.
ER -
ČAJÁNEK, Lukáš, Linda ADLERZ, Vítězslav BRYJA a Ernest ARENAS. WNT unrelated activities in commercially available preparations of recombinant WNT3a. \textit{Journal of Cellular Biochemistry}. 2010, roč.~111, č.~5, s.~1077-1079. ISSN~0730-2312.
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