Wnt2 Regulates Progenitor Proliferation in the Developing
Ventral Midbrain

J 2010

Wnt2 Regulates Progenitor Proliferation in the Developing Ventral Midbrain

SOUSA, Kyle; Carlos VILLAESCUSA; Lukáš ČAJÁNEK; Jennifer ONDR; Goncalo CASTELO-BRANCO et al.

Základní údaje

Originální název

Wnt2 Regulates Progenitor Proliferation in the Developing Ventral Midbrain

Autoři

Vydání

Journal of Biological Chemistry, Bethesda, USA, Amer. Soc. Biochem. Mol. Biol. 2010, 0021-9258

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 5.328

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/10:00047424

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1074/jbc.M109.079822

UT WoS

000275415600037

Klíčová slova anglicky

Wnt2; dopaminergic neuron; ventral midbrain

Změněno: 26. 3. 2012 16:24, prof. Mgr. Vítězslav Bryja, Ph.D.

Anotace

V originále

Wnts are secreted, lipidated proteins that regulate multiple aspects of brain development, including dopaminergic neuron development. In this study, we perform the first purification and signaling analysis of Wnt2 and define the function of Wnt2 in ventral midbrain precursor cultures, as well as in Wnt2-null mice in vivo. We found that purified Wnt2 induces the phosphorylation of both Lrp5/6 and Dvl-2/3, and activates beta-catenin in SN4741 dopaminergic cells. Moreover, purified Wnt2 increases progenitor proliferation, and the number of dopaminergic neurons in ventral midbrain precursor cultures. In agreement with these findings, analysis of the ventral midbrain of developing Wnt2-null mice revealed a decrease in progenitor proliferation and neurogenesis that lead to a decrease in the number of postmitotic precursors and dopaminergic neurons. Collectively, our observations identify Wnt2 as a novel regulator of dopaminergic progenitors and dopaminergic neuron development.

Citovat

SOUSA, Kyle; Carlos VILLAESCUSA; Lukáš ČAJÁNEK; Jennifer ONDR; Goncalo CASTELO-BRANCO; Wytske HOFSTRA; Vítězslav BRYJA; Carina PALMBERG; Tomas BERGMAN; Brandon WAINWRIGHT; Richard LANG a Ernest ARENAS. Wnt2 Regulates Progenitor Proliferation in the Developing Ventral Midbrain. Journal of Biological Chemistry. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2010, roč. 285, č. 10, s. 7246-7253. ISSN 0021-9258. Dostupné z: https://doi.org/10.1074/jbc.M109.079822.

@article{929233,
   author = {Sousa, Kyle and Villaescusa, Carlos and Čajánek, Lukáš and Ondr, Jennifer and CasteloandBranco, Goncalo and Hofstra, Wytske and Bryja, Vítězslav and Palmberg, Carina and Bergman, Tomas and Wainwright, Brandon and Lang, Richard and Arenas, Ernest},
   article_location = {Bethesda, USA},
   article_number = {10},
   doi = {https://doi.org/10.1074/jbc.M109.079822},
   keywords = {Wnt2; dopaminergic neuron; ventral midbrain},
   language = {eng},
   issn = {0021-9258},
   journal = {Journal of Biological Chemistry},
   title = {Wnt2 Regulates Progenitor Proliferation in the Developing Ventral Midbrain},
   volume = {285},
   year = {2010}
}

TY  - JOUR
ID  - 929233
AU  - Sousa, Kyle - Villaescusa, Carlos - Čajánek, Lukáš - Ondr, Jennifer - Castelo-Branco, Goncalo - Hofstra, Wytske - Bryja, Vítězslav - Palmberg, Carina - Bergman, Tomas - Wainwright, Brandon - Lang, Richard - Arenas, Ernest
PY  - 2010
TI  - Wnt2 Regulates Progenitor Proliferation in the Developing Ventral Midbrain
JF  - Journal of Biological Chemistry
VL  - 285
IS  - 10
SP  - 7246-7253
EP  - 7246-7253
PB  - Amer. Soc. Biochem. Mol. Biol.
SN  - 00219258
KW  - Wnt2
KW  - dopaminergic neuron
KW  - ventral midbrain
N2  - Wnts are secreted, lipidated proteins that regulate multiple aspects of brain development, including dopaminergic neuron development. In this study, we perform the first purification and signaling analysis of Wnt2 and define the function of Wnt2 in ventral midbrain precursor cultures, as well as in Wnt2-null mice in vivo. We found that purified Wnt2 induces the phosphorylation of both Lrp5/6 and Dvl-2/3, and activates beta-catenin in SN4741 dopaminergic cells. Moreover, purified Wnt2 increases progenitor proliferation, and the number of dopaminergic neurons in ventral midbrain precursor cultures. In agreement with these findings, analysis of the ventral midbrain of developing Wnt2-null mice revealed a decrease in progenitor proliferation and neurogenesis that lead to a decrease in the number of postmitotic precursors and dopaminergic neurons. Collectively, our observations identify Wnt2 as a novel regulator of dopaminergic progenitors and dopaminergic neuron development.
ER  -

SOUSA, Kyle; Carlos VILLAESCUSA; Lukáš ČAJÁNEK; Jennifer ONDR; Goncalo CASTELO-BRANCO; Wytske HOFSTRA; Vítězslav BRYJA; Carina PALMBERG; Tomas BERGMAN; Brandon WAINWRIGHT; Richard LANG a Ernest ARENAS. Wnt2 Regulates Progenitor Proliferation in the Developing Ventral Midbrain. \textit{Journal of Biological Chemistry}. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2010, roč.~285, č.~10, s.~7246-7253. ISSN~0021-9258. Dostupné z: https://doi.org/10.1074/jbc.M109.079822.

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