Effect of „osmotic stabilizers“and glycerol on yeast cell
envelopes.

J 1973

Effect of „osmotic stabilizers“and glycerol on yeast cell envelopes.

KOPECKÁ, Marie, Augustin SVOBODA a Jaroslav BRICHTA

Základní údaje

Originální název

Effect of „osmotic stabilizers“and glycerol on yeast cell envelopes.

Autoři

KOPECKÁ, Marie, Augustin SVOBODA a Jaroslav BRICHTA

Vydání

Z. Allg. Microbiol, GERMANY, Publisher: AKADEMIE VERLAG GMBH, MUHLENSTRASSE 33-34, D-13187 BERLIN, GERMANY, 1973, 0044-2208

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Organizační jednotka

Lékařská fakulta

UT WoS

A1973Q616700004

Klíčová slova anglicky

yeast cell envelopes; osmotic stabilizers; electron microscopy

Štítky

Práce byla 11x citována ve WOS do 1.3.2011

Změněno: 14. 3. 2011 15:00, prof. MUDr. Marie Kopecká, CSc.

Anotace

V originále

The ultrastructure of cells of Saccharomyces cerevisiae and Schizosaccharomyces pombe was studied after their transfer to solutions of osmotic stabilizers used for maintenance of yeast protoplasts (0.7 m d-mannitol, 0.7 m l-rhamnose, and 0.6 m KCl). After 10 to 30 min incubation in these stabilizers significant changes appeared on the cell surfaces which progressed up to 60 min of incubation. The changes concerned especially the size, shape, and distribution of plasma membrane invaginations, which became deeper; in addition, large impressions were formed. Complementary changes were found in wall ridges which might result in formation of wall protrusions containing areas of separated cytoplasm. Similar changes of cell surfaces appeared after a short incubation of in 10% and 20% glycerol. The changes described are interpreted as a consequence of volume decrease and unbalanced cell wall synthesis after transferring the cells to media of higher osmolarity.

Citovat

KOPECKÁ, Marie, Augustin SVOBODA a Jaroslav BRICHTA. Effect of „osmotic stabilizers“and glycerol on yeast cell envelopes. Z. Allg. Microbiol. GERMANY: Publisher: AKADEMIE VERLAG GMBH, MUHLENSTRASSE 33-34, D-13187 BERLIN, GERMANY, 1973, roč. 13, č. 6, s. 481-487. ISSN 0044-2208.

@article{930217,
   author = {Kopecká, Marie and Svoboda, Augustin and Brichta, Jaroslav},
   article_location = {GERMANY},
   article_number = {6},
   keywords = {yeast cell envelopes; osmotic stabilizers; electron microscopy},
   language = {eng},
   issn = {0044-2208},
   journal = {Z. Allg. Microbiol},
   title = {Effect of „osmotic stabilizers“and glycerol on yeast cell envelopes.},
   url = {http://apps.isiknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=1&SID=W1okoo1b85iLefnnN9I&page=5&doc=43},
   volume = {13},
   year = {1973}
}

TY  - JOUR
ID  - 930217
AU  - Kopecká, Marie - Svoboda, Augustin - Brichta, Jaroslav
PY  - 1973
TI  - Effect of „osmotic stabilizers“and glycerol on yeast cell envelopes.
JF  - Z. Allg. Microbiol
VL  - 13
IS  - 6
SP  - 481-487
EP  - 481-487
PB  - Publisher: AKADEMIE VERLAG GMBH, MUHLENSTRASSE 33-34, D-13187 BERLIN, GERMANY
SN  - 00442208
KW  - yeast cell envelopes
KW  - osmotic stabilizers
KW  - electron microscopy
UR  - http://apps.isiknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=1&SID=W1okoo1b85iLefnnN9I&page=5&doc=43
N2  - The ultrastructure of cells of Saccharomyces cerevisiae and Schizosaccharomyces pombe was studied after their transfer to solutions of osmotic stabilizers used for maintenance of yeast protoplasts (0.7 m d-mannitol, 0.7 m l-rhamnose, and 0.6 m KCl). After 10 to 30 min incubation in these stabilizers significant changes appeared on the cell surfaces which progressed up to 60 min of incubation. The changes concerned especially the size, shape, and distribution of plasma membrane invaginations, which became deeper; in addition, large impressions were formed. Complementary changes were found in wall ridges which might result in formation of wall protrusions containing areas of separated cytoplasm. Similar changes of cell surfaces appeared after a short incubation of in 10% and 20% glycerol. The changes described are interpreted as a consequence of volume decrease and unbalanced cell wall synthesis after transferring the cells to media of higher osmolarity.
ER  -

KOPECKÁ, Marie, Augustin SVOBODA a Jaroslav BRICHTA. Effect of „osmotic stabilizers“and glycerol on yeast cell envelopes. \textit{Z. Allg. Microbiol}. GERMANY: Publisher: AKADEMIE VERLAG GMBH, MUHLENSTRASSE 33-34, D-13187 BERLIN, GERMANY, 1973, roč.~13, č.~6, s.~481-487. ISSN~0044-2208.

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