2011
Cryptochrome 2 necessary for magnetosensitivity of American cockroach Periplaneta americana.
BAZALOVA, Olina, Hana SEHADOVA, Rudolf LOESEL, Tereza VÁLKOVÁ, Přemysl BARTOŠ et. al.Základní údaje
Originální název
Cryptochrome 2 necessary for magnetosensitivity of American cockroach Periplaneta americana.
Název česky
Cryptochrome 2 necessary for magnetosensitivity of American cockroach Periplaneta americana.
Autoři
BAZALOVA, Olina (203 Česká republika), Hana SEHADOVA (203 Česká republika), Rudolf LOESEL (276 Německo), Tereza VÁLKOVÁ (203 Česká republika, domácí), Přemysl BARTOŠ (203 Česká republika, domácí), Markéta KVÍČALOVÁ (203 Česká republika, domácí), Kateřina TOMANOVÁ (203 Česká republika, domácí), Ivo ŠAUMAN (203 Česká republika, domácí), David DOLEZEL (203 Česká republika) a Martin VÁCHA (203 Česká republika, garant, domácí)
Vydání
Orientation & Navigation. Birds Humans & Other Animals. 7th Conference in this Series, 6-9 April 2011, University of Reading, UK, 2011
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
30105 Physiology
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/11:00049747
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova anglicky
magnetoreception cryptochrome insects vision
Změněno: 12. 4. 2011 13:21, doc. RNDr. Martin Vácha, Ph.D.
V originále
Cryptochromes (CRYs) – proteins that have been postulated to generate radical pairs sensitive to geomagnetic field and facilitate light-dependent compass sense of animals – mediate magnetoreception in fruit fly (Gegear et al., 2008; 2010). Using Cry deficient and transgenic flies the authors showed that Drosophila magnetosensitivity relies on both drosophila-like CRY type 1 and vertebrate-like CRY 2. Here, we combined reverse genetics approach with non-conditioning behavioral assay of enhanced movement activity (restlessness) under magnetic North shifts on insect Periplaneta americana. We investigated the impact of cry2 gene silencing (by means of RNA interference). We identified CRY 2 as the only Cryptochrome in Periplaneta, prepared dsRNA and injected into cockroaches. After 15 days, decline CRY mRNA levels (i) together with disruption of circadian rhythms (ii) and loss of the magnetosensory reaction (iii) showed that CRY2 is necessary both for circadian rhythms control and for magnetic sensitivity in Periplaneta. Disrupting the molecular clock itself under constant light or by silencing tim gene did not affect the magnetic sensitivity. Hence, behavioral response to magnetic stimuli was dependent on unsuppressed CRY2 production selectively. Since the assay depends on no learned skills and uses natural intensities of geomagnetic field our findings give evidence of specific role for Cryptochrome 2 in insect magnetoreception.
Česky
Cryptochromes (CRYs) – proteins that have been postulated to generate radical pairs sensitive to geomagnetic field and facilitate light-dependent compass sense of animals – mediate magnetoreception in fruit fly (Gegear et al., 2008; 2010). Using Cry deficient and transgenic flies the authors showed that Drosophila magnetosensitivity relies on both drosophila-like CRY type 1 and vertebrate-like CRY 2. Here, we combined reverse genetics approach with non-conditioning behavioral assay of enhanced movement activity (restlessness) under magnetic North shifts on insect Periplaneta americana. We investigated the impact of cry2 gene silencing (by means of RNA interference). We identified CRY 2 as the only Cryptochrome in Periplaneta, prepared dsRNA and injected into cockroaches. After 15 days, decline CRY mRNA levels (i) together with disruption of circadian rhythms (ii) and loss of the magnetosensory reaction (iii) showed that CRY2 is necessary both for circadian rhythms control and for magnetic sensitivity in Periplaneta. Disrupting the molecular clock itself under constant light or by silencing tim gene did not affect the magnetic sensitivity. Hence, behavioral response to magnetic stimuli was dependent on unsuppressed CRY2 production selectively. Since the assay depends on no learned skills and uses natural intensities of geomagnetic field our findings give evidence of specific role for Cryptochrome 2 in insect magnetoreception.
Návaznosti
| GC206/07/J041, projekt VaV |
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| MSM0021622416, záměr |
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