2011
Hemolymph clotting and immunity.
THEOPOLD, Ulrich; Zhi WANG; Pavel DOBEŠ a Pavel HYRŠLZákladní údaje
Originální název
Hemolymph clotting and immunity.
Autoři
THEOPOLD, Ulrich; Zhi WANG; Pavel DOBEŠ a Pavel HYRŠL
Vydání
52nd Annual Drosophila Research Conference, 2011
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
30102 Immunology
Stát vydavatele
Spojené státy
Utajení
není předmětem státního či obchodního tajemství
Označené pro přenos do RIV
Ano
Kód RIV
RIV/00216224:14310/11:00049751
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova česky
Drosophila; Heterorhabditis; imunita; srážení hemolymfy; eikosanoidy
Klíčová slova anglicky
Drosophila; Heterorhabditis; immunity; clotting; eicosanoids
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 9. 3. 2012 08:40, doc. RNDr. Pavel Hyršl, Ph.D.
Anotace
V originále
We are interested in the clotting of insect hemolymph. Clotting in Drosophila involves the precipitation of clotting factors and crosslinking of the clot. The latter process is mediated through the activity of the two enzymes transglutaminase and phenoloxidase. Our recent work shows that in addition to sealing wounds clotting also has a major function in protecting Drosophila larvae from infections. Larvae with reduced expression of either clotting factors or transglutaminase are more susceptible to infections with certain bacteria. The most significant effects were observed when we infected larvae using a natural infection model involving entomopathogenic nematodes and their associated bacteria. Our most recent results show that while single mutants in several previously characterized immune pathways (such as the imd pathway) behave similar to normal larvae, double mutants succumb faster to the infection. For example Bc/imd double mutants show immune defects. Using inhibitors of eicosanoid biosynthesis and knockdown lines specific for candidate genes involved in eicosanoid production we also provide evidence that lipid mediators might play a role in insect immunity. Finally, we also show that blood clots in mammals contribute to immunity.
Návaznosti
| GP206/09/P470, projekt VaV |
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