Metallochelating liposomes with associated lipophilised
norAbuMDP as biocompatible platform for construction of
vaccines with recombinant His-tagged antigens: Preparation,
structural study and immune response towards rHsp90

J 2011

Metallochelating liposomes with associated lipophilised norAbuMDP as biocompatible platform for construction of vaccines with recombinant His-tagged antigens: Preparation, structural study and immune response towards rHsp90

MAŠEK, Josef; Eliška BARTHELDYOVÁ; Pavlína TURÁNEK KNÖTIGOVÁ; Michaela ŠKRABALOVÁ; Zina KORVASOVÁ et al.

Základní údaje

Originální název

Metallochelating liposomes with associated lipophilised norAbuMDP as biocompatible platform for construction of vaccines with recombinant His-tagged antigens: Preparation, structural study and immune response towards rHsp90

Autoři

Vydání

JOURNAL OF CONTROLLED RELEASE, Elsevier, 2011, 0168-3659

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30104 Pharmacology and pharmacy

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 6.499

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/11:00052337

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.1016/j.jconrel.2011.01.016

UT WoS

000291845600012

Klíčová slova anglicky

Recombinant vaccine; Liposome; Heat shock; protein; Hsp90; Muramyl; dipeptide; Candida albicans

Příznaky

Mezinárodní význam

Změněno: 12. 5. 2016 10:03, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Hsp90-CA is present in cell wall of Candida pseudohyphae or hyphae-typical pathogenic morphotype for both systemic and mucosal Candida infections. Heat shock protein from Candida albicans (hsp90-CA) is an important target for protective antibodies during disseminated candidiasis of experimental mice and human. His-tagged protein rHsp90 was prepared and used as the antigen for preparation of experimental recombinant liposomal vaccine. Nickel-chelating liposomes (the size around 100 nm) were prepared from the mixture of egg phosphatidyl choline and nickel-chelating lipid DOGS-NTA-Ni (molar ratio 95:5%) by hydration of lipid film and extrusion methods. New non-pyrogenic hydrophobised derivative of MDP (C18-O-6-norAbuMDP) was incorporated into liposomes as adjuvans. rHsp90 was attached onto the surface of metallochelating liposomes by metallochelating bond and the structure of these proteoliposomes was studied by dynamic light scattering, AF microscopy, TEM and GPC. The liposomes with surface-exposed C18-O-6-norAbuMDP were well recognised and phagocyted by human dendritic cells in vitro. In vivo the immune response towards this experimental vaccine applied in mice (i.d.) demonstrated both TH1 and TH2 response comparable to FCA, but without any side effects. Metallochelating liposomes with lipophilic derivatives of muramyl dipeptide represent a new biocompatible platform for construction of experimental recombinant vaccines and drug-targeting systems.

Návaznosti

2B06058, projekt VaV

Název: Bezpečná biotechnologie alotransplantovaných hematopoetických kmenových buněk u vybraných solidních a hematologických nádorů jako adoptivní imunoterapie (Akronym: NPVII11001)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Bezpečná biotechnologie alotransplatovaných hematopoetických kmenových buněk u vybraných solidních a hematologických nádorů jako adoptivní imunoterapie

Citovat

MAŠEK, Josef; Eliška BARTHELDYOVÁ; Pavlína TURÁNEK KNÖTIGOVÁ; Michaela ŠKRABALOVÁ; Zina KORVASOVÁ; Jana PLOCKOVÁ; Štěpán KOUDELKA; Petra ŠKODOVÁ; Pavel KULICH; Michal KŘUPKA; Kateřina ZACHOVÁ; Lýdie CZERNEKOVÁ; Milada HORYNOVÁ; Irena KRATOCHVÍLOVÁ; Andrew D. MILLER; Daniel ZÝKA; Jaroslav MICHÁLEK; Jana VRBKOVÁ; Marek ŠEBELA; Miroslav LEDVINA; Milan RAŠKA a Jaroslav TURÁNEK. Metallochelating liposomes with associated lipophilised norAbuMDP as biocompatible platform for construction of vaccines with recombinant His-tagged antigens: Preparation, structural study and immune response towards rHsp90. JOURNAL OF CONTROLLED RELEASE. Elsevier, 2011, roč. 151, č. 2, s. 193-201. ISSN 0168-3659. Dostupné z: https://doi.org/10.1016/j.jconrel.2011.01.016.

@article{939177,
   author = {Mašek, Josef and Bartheldyová, Eliška and Turánek Knötigová, Pavlína and Škrabalová, Michaela and Korvasová, Zina and Plocková, Jana and Koudelka, Štěpán and Škodová, Petra and Kulich, Pavel and Křupka, Michal and Zachová, Kateřina and Czerneková, Lýdie and Horynová, Milada and Kratochvílová, Irena and Miller, Andrew D. and Zýka, Daniel and Michálek, Jaroslav and Vrbková, Jana and Šebela, Marek and Ledvina, Miroslav and Raška, Milan and Turánek, Jaroslav},
   article_number = {2},
   doi = {https://doi.org/10.1016/j.jconrel.2011.01.016},
   keywords = {Recombinant vaccine; Liposome; Heat shock; protein; Hsp90; Muramyl; dipeptide; Candida albicans},
   language = {eng},
   issn = {0168-3659},
   journal = {JOURNAL OF CONTROLLED RELEASE},
   title = {Metallochelating liposomes with associated lipophilised norAbuMDP as biocompatible platform for construction of vaccines with recombinant His-tagged antigens: Preparation, structural study and immune response towards rHsp90},
   volume = {151},
   year = {2011}
}

TY  - JOUR
ID  - 939177
AU  - Mašek, Josef - Bartheldyová, Eliška - Turánek Knötigová, Pavlína - Škrabalová, Michaela - Korvasová, Zina - Plocková, Jana - Koudelka, Štěpán - Škodová, Petra - Kulich, Pavel - Křupka, Michal - Zachová, Kateřina - Czerneková, Lýdie - Horynová, Milada - Kratochvílová, Irena - Miller, Andrew D. - Zýka, Daniel - Michálek, Jaroslav - Vrbková, Jana - Šebela, Marek - Ledvina, Miroslav - Raška, Milan - Turánek, Jaroslav
PY  - 2011
TI  - Metallochelating liposomes with associated lipophilised norAbuMDP as biocompatible platform for construction of vaccines with recombinant His-tagged antigens: Preparation, structural study and immune response towards rHsp90
JF  - JOURNAL OF CONTROLLED RELEASE
VL  - 151
IS  - 2
SP  - 193-201
EP  - 193-201
PB  - Elsevier
SN  - 01683659
KW  - Recombinant vaccine
KW  - Liposome
KW  - Heat shock
KW  - protein
KW  - Hsp90
KW  - Muramyl
KW  - dipeptide
KW  - Candida albicans
N2  - Hsp90-CA is present in cell wall of Candida pseudohyphae or hyphae-typical pathogenic morphotype for both systemic and mucosal Candida infections. Heat shock protein from Candida albicans (hsp90-CA) is an important target for protective antibodies during disseminated candidiasis of experimental mice and human. His-tagged protein rHsp90 was prepared and used as the antigen for preparation of experimental recombinant liposomal vaccine. Nickel-chelating liposomes (the size around 100 nm) were prepared from the mixture of egg phosphatidyl choline and nickel-chelating lipid DOGS-NTA-Ni (molar ratio 95:5%) by hydration of lipid film and extrusion methods. New non-pyrogenic hydrophobised derivative of MDP (C18-O-6-norAbuMDP) was incorporated into liposomes as adjuvans. rHsp90 was attached onto the surface of metallochelating liposomes by metallochelating bond and the structure of these proteoliposomes was studied by dynamic light scattering, AF microscopy, TEM and GPC. The liposomes with surface-exposed C18-O-6-norAbuMDP were well recognised and phagocyted by human dendritic cells in vitro. In vivo the immune response towards this experimental vaccine applied in mice (i.d.) demonstrated both TH1 and TH2 response comparable to FCA, but without any side effects. Metallochelating liposomes with lipophilic derivatives of muramyl dipeptide represent a new biocompatible platform for construction of experimental recombinant vaccines and drug-targeting systems.
ER  -

MAŠEK, Josef; Eliška BARTHELDYOVÁ; Pavlína TURÁNEK KNÖTIGOVÁ; Michaela ŠKRABALOVÁ; Zina KORVASOVÁ; Jana PLOCKOVÁ; Štěpán KOUDELKA; Petra ŠKODOVÁ; Pavel KULICH; Michal KŘUPKA; Kateřina ZACHOVÁ; Lýdie CZERNEKOVÁ; Milada HORYNOVÁ; Irena KRATOCHVÍLOVÁ; Andrew D. MILLER; Daniel ZÝKA; Jaroslav MICHÁLEK; Jana VRBKOVÁ; Marek ŠEBELA; Miroslav LEDVINA; Milan RAŠKA a Jaroslav TURÁNEK. Metallochelating liposomes with associated lipophilised norAbuMDP as biocompatible platform for construction of vaccines with recombinant His-tagged antigens: Preparation, structural study and immune response towards rHsp90. \textit{JOURNAL OF CONTROLLED RELEASE}. Elsevier, 2011, roč.~151, č.~2, s.~193-201. ISSN~0168-3659. Dostupné z: https://doi.org/10.1016/j.jconrel.2011.01.016.

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