2011
ACID-BASE PROPERTIES OF CYTOSINE-CONTAINING OLIGONUCLEOTIDES
HOLUBOVÁ, Sylvie; Zdeňka BALCAROVÁ; Přemysl LUBAL a Libuše TRNKOVÁZákladní údaje
Originální název
ACID-BASE PROPERTIES OF CYTOSINE-CONTAINING OLIGONUCLEOTIDES
Autoři
HOLUBOVÁ, Sylvie; Zdeňka BALCAROVÁ; Přemysl LUBAL a Libuše TRNKOVÁ
Vydání
XXI INTERNATIONAL SYMPOSIUM ON BIOELECTROCHEMISTRY AND BIOENERGETICS, 2011
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
10403 Physical chemistry
Stát vydavatele
Polsko
Utajení
není předmětem státního či obchodního tajemství
Označené pro přenos do RIV
Ano
Kód RIV
RIV/00216224:14310/11:00052407
Organizační jednotka
Přírodovědecká fakulta
ISBN
978-83-62139-23-1
Klíčová slova anglicky
ODN;cytosine;UV-Vis;spectrophotometry;protonation constant
Štítky
Změněno: 13. 4. 2012 12:34, prof. RNDr. Libuše Trnková, CSc.
Anotace
V originále
The protonation equilibria1 of short-chain cytosine homo-oligodeoxyribonucleotides (ODNs) were studied by means of voltammetry and UV-Vis spectra. The work was aimed at ssDNA fragments with different length of the ODN chain. The shortest and longest ODNs were dC3 and dC18, respectively. In order to know the thermodynamic stability of ODNs, the effect of temperature and ionic strength was studied. The protonation constants were calculated by using a hard model (OPIUM or EQUISPEC) 2,3. It was found that the prolongation of the ODN chain supports the formation of more non-canonical bonds in the hemiprotonated (C+-C) pair (“i-motif”). While the exothermic protonation of nucleotides (CMP or dCMP) does not differ, the thermodynamic parameters change for ODNs as a consequence of the existence of an “i-motif” confirmed also by elimination voltammetry with linear scan (EVLS)4. These facts are important for the knowledge of the secondary structure of ODNs containing C nucleobase.
Návaznosti
| LC06035, projekt VaV |
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| MSM0021622412, záměr |
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| MUNI/A/0992/2009, interní kód MU |
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