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@proceedings{940633,
author = {Holubová, Sylvie and Balcarová, Zdeňka and Lubal, Přemysl and Trnková, Libuše},
booktitle = {XXI INTERNATIONAL SYMPOSIUM ON BIOELECTROCHEMISTRY AND BIOENERGETICS},
keywords = {ODN;cytosine;UV-Vis;spectrophotometry;protonation constant},
language = {eng},
isbn = {978-83-62139-23-1},
title = {ACID-BASE PROPERTIES OF CYTOSINE-CONTAINING OLIGONUCLEOTIDES},
year = {2011}
}
TY - CONF
ID - 940633
AU - Holubová, Sylvie - Balcarová, Zdeňka - Lubal, Přemysl - Trnková, Libuše
PY - 2011
TI - ACID-BASE PROPERTIES OF CYTOSINE-CONTAINING OLIGONUCLEOTIDES
SN - 9788362139231
KW - ODN;cytosine;UV-Vis;spectrophotometry;protonation constant
N2 - The protonation equilibria1 of short-chain cytosine homo-oligodeoxyribonucleotides (ODNs) were studied by means of voltammetry and UV-Vis spectra. The work was aimed at ssDNA fragments with different length of the ODN chain. The shortest and longest ODNs were dC3 and dC18, respectively. In order to know the thermodynamic stability of ODNs, the effect of temperature and ionic strength was studied. The protonation constants were calculated by using a hard model (OPIUM or EQUISPEC) 2,3. It was found that the prolongation of the ODN chain supports the formation of more non-canonical bonds in the hemiprotonated (C+-C) pair (“i-motif”). While the exothermic protonation of nucleotides (CMP or dCMP) does not differ, the thermodynamic parameters change for ODNs as a consequence of the existence of an “i-motif” confirmed also by elimination voltammetry with linear scan (EVLS)4. These facts are important for the knowledge of the secondary structure of ODNs containing C nucleobase.
ER -
HOLUBOVÁ, Sylvie, Zdeňka BALCAROVÁ, Přemysl LUBAL a Libuše TRNKOVÁ. ACID-BASE PROPERTIES OF CYTOSINE-CONTAINING OLIGONUCLEOTIDES. In \textit{XXI INTERNATIONAL SYMPOSIUM ON BIOELECTROCHEMISTRY AND BIOENERGETICS}. 2011. ISBN~978-83-62139-23-1.
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