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@article{943487, author = {Jing, Ying and Machoň, Ondřej and Hampl, Aleš and Dvořák, Petr and Xing, Ying and Krauss, Stefan}, article_number = {5}, doi = {http://dx.doi.org/10.1007/s10571-011-9669-2}, keywords = {Mouse; embryonic stem cell; Embryonic forebrain; Stem cell; differentiation; Wnt signaling}, language = {eng}, issn = {0272-4340}, journal = {Cellular and Molecular Neurobiology}, title = {In Vitro Differentiation of Mouse Embryonic Stem Cells into Neurons of the Dorsal Forebrain}, volume = {31}, year = {2011} }
TY - JOUR ID - 943487 AU - Jing, Ying - Machoň, Ondřej - Hampl, Aleš - Dvořák, Petr - Xing, Ying - Krauss, Stefan PY - 2011 TI - In Vitro Differentiation of Mouse Embryonic Stem Cells into Neurons of the Dorsal Forebrain JF - Cellular and Molecular Neurobiology VL - 31 IS - 5 SP - 715-727 EP - 715-727 PB - Springer SN - 02724340 KW - Mouse KW - embryonic stem cell KW - Embryonic forebrain KW - Stem cell KW - differentiation KW - Wnt signaling N2 - Pluripotent embryonic stem cells (ESCs) are able to differentiate into all cell types in the organism including cortical neurons. To follow the dynamic generation of progenitors of the dorsal forebrain in vitro, we generated ESCs from D6-GFP mice in which GFP marks neocortical progenitors and neurons after embryonic day (E) 10.5. We used several cell culture protocols for differentiation of ESCs into progenitors and neurons of the dorsal forebrain. In cell culture, GFP-positive cells were induced under differentiation conditions in quickly formed embryoid bodies (qEBs) after 10–12 day incubation. Activation of Wnt signaling during ESC differentiation further stimulated generation of D6-GFP-positive cortical cells. In contrast, differentiation protocols using normal embryoid bodies (nEBs) yielded only a few D6-GFPpositive cells. Gene expression analysis revealed that multiple components of the canonical Wnt signaling pathway were expressed during the development of embryoid bodies. As shown by immunohistochemistry and quantitative qRT-PCR, D6-GFP-positive cells from qEBs expressed genes that are characteristic for the dorsal forebrain such as Pax6, Dach1, Tbr1, Tbr2, or Sox5. qEBs culture allowed the formation of a D6-GFP positive pseudo-polarized neuroepithelium with the characteristic presence of N-cadherin at the apical pole resembling the structure of the developing neocortex. ER -
JING, Ying, Ondřej MACHOŇ, Aleš HAMPL, Petr DVOŘÁK, Ying XING and Stefan KRAUSS. In Vitro Differentiation of Mouse Embryonic Stem Cells into Neurons of the Dorsal Forebrain. \textit{Cellular and Molecular Neurobiology}. Springer, 2011, vol.~31, No~5, p.~715-727. ISSN~0272-4340. Available from: https://dx.doi.org/10.1007/s10571-011-9669-2.
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