2011
Oligonucleotide-based Array CGH as a Diagnostic Tool in Multiple Myeloma Patients
SMETANA, Jan; Jan FRÖHLICH; Vladimíra VRANOVÁ; Aneta MIKULÁŠOVÁ; Petr KUGLÍK et al.Základní údaje
Originální název
Oligonucleotide-based Array CGH as a Diagnostic Tool in Multiple Myeloma Patients
Název česky
Využití techniky komparativní genomové hybridizace na oligonukleotidových čipech jako diagnostického nástroje u pacientů s mnohočetným myelomem
Autoři
Vydání
Klinická onkologie, 2011, 0862-495X
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
30200 3.2 Clinical medicine
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Označené pro přenos do RIV
Ano
Kód RIV
RIV/00216224:14110/11:00049842
Organizační jednotka
Lékařská fakulta
Klíčová slova anglicky
multiple myeloma; array-CGH; cytogenetics
Příznaky
Recenzováno
Změněno: 30. 9. 2011 12:08, Mgr. Michal Petr
Anotace
V originále
Multiple myeloma (MM) is a hematological disease caused by malignant proliferation of clonal plasma cells (PCs) known for its clinical and biological heterogeneity. Identification of chromosomal changes in genome of PCs plays a key role in MM pathogenesis and is supposed to have important prognostic significance for MM patients. There are two major genetic entities in MM. Hyperdiploid tumors (H-MM), which include about 50% of MM tumors, often have multiple trisomies involving chromosomes 3, 5, 7, 9, 11, 15, 19, and 21 and a substantially lower prevalence of IgH translocations. Nearly half of tumors are non-hyperdiploid (NH-MM), and mostly have one of five recurrent IgH translocations: 11q13 (CCND1), 6p21 (CCND3), 16q23 (MAF), 20q12 (MAFB), and 4p16 (FGFR3 and MMSET). The development and expanded use of new technologies, such as genome-wide array-based comparative genomic hybridization (aCGH) has accelerated genomic research in MM. This technique is a powerful tool to globally analyze recurrent copy number changes in tumor genome in a single reaction and to study cancer biology and clinical behaviors. It widely overcame routinely used cytogenetic techniques (G-banding, FISH) both in minimal resolution of chromosomal changes and amount of obtained genomic data important for further analyses and clinical applications. Array CGH technique is now used to better understanding of molecular phenotypes, sensitivity to particular chemotherapeutic agents, and prognosis of these diseases. This paper brings brief literature and methodic overview of oligonucleotide-based array-CGH technique in MM diagnosis.
Návaznosti
| GAP304/10/1395, projekt VaV |
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| LC06027, projekt VaV |
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| MSM0021622434, záměr |
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| NS10207, projekt VaV |
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| NS10406, projekt VaV |
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