Antibacterial agents in innate immunity: comparison of vertebrates and invertebrates

VOJTEK, Libor, Pavel DOBEŠ a Pavel HYRŠL. Antibacterial agents in innate immunity: comparison of vertebrates and invertebrates. In Workshop on Animal Physiology and Immunology. 2011.

Základní údaje

• Originální název: Antibacterial agents in innate immunity: comparison of vertebrates and invertebrates
• Autoři: VOJTEK, Libor (203 Česká republika, garant, domácí), Pavel DOBEŠ (203 Česká republika, domácí) a Pavel HYRŠL (203 Česká republika, domácí).
• Vydání: Workshop on Animal Physiology and Immunology, 2011.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Konferenční abstrakt
• Obor: 30102 Immunology
• Stát vydavatele: Česká republika
• Utajení: není předmětem státního či obchodního tajemství
• Kód RIV: RIV/00216224:14310/11:00049851
• Organizační jednotka: Přírodovědecká fakulta
• Klíčová slova česky: vertebrates; invertebrates; Escherichia coli K12; Photorhabdus luminescens; bioluminescence; antibacterial peptides; antibacterial assay
• Klíčová slova anglicky: vertebrates; invertebrates; Escherichia coli K12; Photorhabdus luminescens; bioluminescence; antibacterial peptides; antibacterial assay

Anotace

Innate immunity of both vertebrates and invertebrates contents number of antibacterial mechanisms. In invertebrates there is lysozyme and several different antibacterial peptides which are divided according to their stucture to four main groups called linear a-helical, cysteine-stabilized, proline-rich and glycine-rich inducible antimicrobial peptides. In vertebrates antibacterial components cooperate with antibodies, complement cascade and myeloperoxidase activity. Nowadays, many common methods for determination of non-specific innate immune parameters are known in vertebrates, nevertheless there is not so large variety of them in invertebrates. Therefore we tried to apply to invertebrates the bioluminescent bacteria-based assay which is commonly used for complement determination in vertebrates. Bioluminescent bacteria are frequent in salt water environment, but there is the only one genus of soil bacteria with natural bioluminescence – Photorhabdus sp. These symbiotic bacteria occur in the gut of entomopathogenic nematodes which are obligate insect parasites. Except of P. luminescens we used the artificial bioluminescent bacterium - genetically modified Escherichia coli K12 that carries Photorhabdus genes for bioluminescence. Bioluminescence is directly connected to the kinetics of bacterial viability. After addition of sample (silkworm or waxmoth haemolymph, fish or human plasma) we observed decrease in viability of bacteria expressed by reduced bioluminescent signal. Using different conditions of bioluminescence assay and inhibitors of particular immune effectors, we optimised this assay for antibacterial activity measurement of specific parts of immune system both in vertebrates and invertebrates. We found out that the level of antibacterial activity of complement in vertebrates is similar to the effect of antibacterial peptides in insect haemolymph. Moreover invertebrate immune system was in several reactions more effective than complement cascade in vertebrates. Our research is supported by grant from Grant Agency of Czech Republic (GA206/09/P470).

Anotace česky

Innate immunity of both vertebrates and invertebrates contents number of antibacterial mechanisms. In invertebrates there is lysozyme and several different antibacterial peptides which are divided according to their stucture to four main groups called linear a-helical, cysteine-stabilized, proline-rich and glycine-rich inducible antimicrobial peptides. In vertebrates antibacterial components cooperate with antibodies, complement cascade and myeloperoxidase activity. Nowadays, many common methods for determination of non-specific innate immune parameters are known in vertebrates, nevertheless there is not so large variety of them in invertebrates. Therefore we tried to apply to invertebrates the bioluminescent bacteria-based assay which is commonly used for complement determination in vertebrates. Bioluminescent bacteria are frequent in salt water environment, but there is the only one genus of soil bacteria with natural bioluminescence – Photorhabdus sp. These symbiotic bacteria occur in the gut of entomopathogenic nematodes which are obligate insect parasites. Except of P. luminescens we used the artificial bioluminescent bacterium - genetically modified Escherichia coli K12 that carries Photorhabdus genes for bioluminescence. Bioluminescence is directly connected to the kinetics of bacterial viability. After addition of sample (silkworm or waxmoth haemolymph, fish or human plasma) we observed decrease in viability of bacteria expressed by reduced bioluminescent signal. Using different conditions of bioluminescence assay and inhibitors of particular immune effectors, we optimised this assay for antibacterial activity measurement of specific parts of immune system both in vertebrates and invertebrates. We found out that the level of antibacterial activity of complement in vertebrates is similar to the effect of antibacterial peptides in insect haemolymph. Moreover invertebrate immune system was in several reactions more effective than complement cascade in vertebrates. Our research is supported by grant from Grant Agency of Czech Republic (GA206/09/P470).

Návaznosti

• GP206/09/P470, projekt VaV: Název: Vliv inhibitorů biosyntézy eikosanoidů na imunitu zavíječe voskového Galleria mellonella

Investor: Grantová agentura ČR, Vliv inhibitorů biosyntézy eikonsanoidů na imunitu zavíječe voskového Galleria mellonella