| PRUDNIKOVA, Tanyana, Radka CHALOUPKOVÁ, Yukari SATO, Yuji NAGATA, O. DEGTJARIK, Michal KUTÝ, P. REZACOVA, Jiří DAMBORSKÝ a Smatanova I. KUTA-SMATANOVÁ. Development of a Crystallization Protocol for the DbeA1 Variant of Novel Haloalkane Dehalogenase from Bradyrhizobium elkani USDA94. Crystal Growth & Design. 2011, roč. 11, č. 2, s. 516-519. ISSN 1528-7483. Dostupné z: https://dx.doi.org/10.1021/cg1013363. |
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@article{947493,
author = {Prudnikova, Tanyana and Chaloupková, Radka and Sato, Yukari and Nagata, Yuji and Degtjarik, O. and Kutý, Michal and Rezacova, P. and Damborský, Jiří and KutaandSmatanová, Smatanova I.},
article_number = {2},
doi = {http://dx.doi.org/10.1021/cg1013363},
keywords = {Crystallization; X-ray;Haloalkane dehalogenases},
language = {eng},
issn = {1528-7483},
journal = {Crystal Growth & Design},
title = {Development of a Crystallization Protocol for the DbeA1 Variant of Novel Haloalkane Dehalogenase from Bradyrhizobium elkani USDA94},
volume = {11},
year = {2011}
}
TY - JOUR
ID - 947493
AU - Prudnikova, Tanyana - Chaloupková, Radka - Sato, Yukari - Nagata, Yuji - Degtjarik, O. - Kutý, Michal - Rezacova, P. - Damborský, Jiří - Kuta-Smatanová, Smatanova I.
PY - 2011
TI - Development of a Crystallization Protocol for the DbeA1 Variant of Novel Haloalkane Dehalogenase from Bradyrhizobium elkani USDA94
JF - Crystal Growth & Design
VL - 11
IS - 2
SP - 516-519
EP - 516-519
SN - 15287483
KW - Crystallization
KW - X-ray;Haloalkane dehalogenases
N2 - The DbeA1 variant of a novel haloalkane dehalogenaseDbeA (EC 3.8.1.5) from Bradyrhizobium elkani USDA94 was constructed to study the structure-function relationships between DbjA and DbeA enzymes. A DbeA1 variant carries a unique fragment of nine amino acids transplanted from the sequentially closely related enzyme DbjA from Bradyrhizobium japonicum USDA110 to DbeA. Here we report development of the crystallization protocol for DbeA1 and soaking experiments with the ligands 1-fluoropentane and 1,3-dichloropropane.
ER -
PRUDNIKOVA, Tanyana, Radka CHALOUPKOVÁ, Yukari SATO, Yuji NAGATA, O. DEGTJARIK, Michal KUTÝ, P. REZACOVA, Jiří DAMBORSKÝ a Smatanova I. KUTA-SMATANOVÁ. Development of a Crystallization Protocol for the DbeA1 Variant of Novel Haloalkane Dehalogenase from Bradyrhizobium elkani USDA94. \textit{Crystal Growth \&{} Design}. 2011, roč.~11, č.~2, s.~516-519. ISSN~1528-7483. Dostupné z: https://dx.doi.org/10.1021/cg1013363.
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