Crystallization and Crystallographic Analysis of the
Rhodococcus rhodochrous NCIMB 13064 DhaA Mutant DhaA31 and its
Complex with 1,2,3-Trichloropropane

J 2011

Crystallization and Crystallographic Analysis of the Rhodococcus rhodochrous NCIMB 13064 DhaA Mutant DhaA31 and its Complex with 1,2,3-Trichloropropane

LAHODA, M.; Radka CHALOUPKOVÁ; Alena STSIAPANAVA; Jiří DAMBORSKÝ; I. SMATANOVA-KUTA et al.

Základní údaje

Originální název

Crystallization and Crystallographic Analysis of the Rhodococcus rhodochrous NCIMB 13064 DhaA Mutant DhaA31 and its Complex with 1,2,3-Trichloropropane

Autoři

LAHODA, M.; Radka CHALOUPKOVÁ; Alena STSIAPANAVA; Jiří DAMBORSKÝ a I. SMATANOVA-KUTA

Vydání

Acta Crystallographica F, 2011, 1744-3091

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 0.506

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/11:00049447

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1107/S1744309111001254

UT WoS

000288225100022

Klíčová slova anglicky

Crystallization; Rhodococcus rhodochrous; DhaA Mutant

Štítky

AKR, rivok

Změněno: 20. 4. 2012 10:37, Ing. Andrea Mikešková

Anotace

V originále

Haloalkane dehalogenases hydrolyze carbon-halogen bonds in a wide range of halogenated aliphatic compounds. The potential use of haloalkane dehalogenases in bioremediation applications has stimulated intensive investigation of these enzymes and their engineering. The mutant DhaA31 was constructed to degrade the anthropogenic compound 1,2,3-trichloropropane (TCP) using a new strategy. This strategy enhances activity towards TCP by decreasing the accessibility of the active site to water molecules, thereby promoting formation of the activated complex. The structure of DhaA31 will help in understanding the structure-function relationships involved in the improved dehalogenation of TCP. The mutant protein DhaA31 was crystallized by the sitting-drop vapour-diffusion technique and crystals of DhaA31 in complex with TCP were obtained using soaking experiments. Both crystals belonged to the triclinic space group P1.

Návaznosti

ED0001/01/01, projekt VaV

Název: CETOCOEN

IAA401630901, projekt VaV

Název: Evoluce substrátové specifity u enzymů aktivních s xenobiotickými látkami

Investor: Akademie věd ČR, Evoluce substrátové specifity u enzymů aktivních s xenobiotickými látkami

LC06010, projekt VaV

Název: Centrum biokatalýzy a biotransformací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Centrum biokatalýzy a biotransformací

MSM0021622412, záměr

Název: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Akronym: INCHEMBIOL)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální , regionální a lokální úrovni

Citovat

LAHODA, M.; Radka CHALOUPKOVÁ; Alena STSIAPANAVA; Jiří DAMBORSKÝ a I. SMATANOVA-KUTA. Crystallization and Crystallographic Analysis of the Rhodococcus rhodochrous NCIMB 13064 DhaA Mutant DhaA31 and its Complex with 1,2,3-Trichloropropane. Acta Crystallographica F. 2011, roč. 67, č. 67, s. 397-400. ISSN 1744-3091. Dostupné z: https://doi.org/10.1107/S1744309111001254.

@article{947513,
   author = {Lahoda, M. and Chaloupková, Radka and Stsiapanava, Alena and Damborský, Jiří and SmatanovaandKuta, I.},
   article_number = {67},
   doi = {https://doi.org/10.1107/S1744309111001254},
   keywords = {Crystallization; Rhodococcus rhodochrous; DhaA Mutant},
   language = {eng},
   issn = {1744-3091},
   journal = {Acta Crystallographica F},
   title = {Crystallization and Crystallographic Analysis of the Rhodococcus rhodochrous NCIMB 13064 DhaA Mutant DhaA31 and its Complex with 1,2,3-Trichloropropane},
   volume = {67},
   year = {2011}
}

TY  - JOUR
ID  - 947513
AU  - Lahoda, M. - Chaloupková, Radka - Stsiapanava, Alena - Damborský, Jiří - Smatanova-Kuta, I.
PY  - 2011
TI  - Crystallization and Crystallographic Analysis of the Rhodococcus rhodochrous NCIMB 13064 DhaA Mutant DhaA31 and its Complex with 1,2,3-Trichloropropane
JF  - Acta Crystallographica F
VL  - 67
IS  - 67
SP  - 397-400
EP  - 397-400
SN  - 17443091
KW  - Crystallization
KW  - Rhodococcus rhodochrous
KW  - DhaA Mutant
N2  - Haloalkane dehalogenases hydrolyze carbon-halogen bonds in a wide range of halogenated aliphatic compounds. The potential use of haloalkane dehalogenases in bioremediation applications has stimulated intensive investigation of these enzymes and their engineering. The mutant DhaA31 was constructed to degrade the anthropogenic compound 1,2,3-trichloropropane (TCP) using a new strategy. This strategy enhances activity towards TCP by decreasing the accessibility of the active site to water molecules, thereby promoting formation of the activated complex. The structure of DhaA31 will help in understanding the structure-function relationships involved in the improved dehalogenation of TCP. The mutant protein DhaA31 was crystallized by the sitting-drop vapour-diffusion technique and crystals of DhaA31 in complex with TCP were obtained using soaking experiments. Both crystals belonged to the triclinic space group P1.
ER  -

LAHODA, M.; Radka CHALOUPKOVÁ; Alena STSIAPANAVA; Jiří DAMBORSKÝ a I. SMATANOVA-KUTA. Crystallization and Crystallographic Analysis of the Rhodococcus rhodochrous NCIMB 13064 DhaA Mutant DhaA31 and its Complex with 1,2,3-Trichloropropane. \textit{Acta Crystallographica F}. 2011, roč.~67, č.~67, s.~397-400. ISSN~1744-3091. Dostupné z: https://doi.org/10.1107/S1744309111001254.

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