| CECIONI, Samy, Sophie FAURE, Ulrich DARBOST, Isabelle BONNAMOUR, Helene PARROT-LOPEZ, Olivier ROY, Claude TAILLEFUMIER, Michaela WIMMEROVÁ, Jean-Pierre PRALY, Anne IMBERTY a Sebastien VIDAL. Selectivity among Two Lectins: Probing the Effect of Topology, Multivalency and Flexibility of “Clicked” Multivalent Glycoclusters. Chemistry - A European Journal. WILEY-VCH, 2011, roč. 17, č. 7, s. 2146–2159. ISSN 0947-6539. Dostupné z: https://dx.doi.org/10.1002/chem.201002635. |
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@article{947554,
author = {Cecioni, Samy and Faure, Sophie and Darbost, Ulrich and Bonnamour, Isabelle and ParrotandLopez, Helene and Roy, Olivier and Taillefumier, Claude and Wimmerová, Michaela and Praly, JeanandPierre and Imberty, Anne and Vidal, Sebastien},
article_number = {7},
doi = {http://dx.doi.org/10.1002/chem.201002635},
keywords = {lectin; glycocluster;SPR;ITC},
language = {eng},
issn = {0947-6539},
journal = {Chemistry - A European Journal},
title = {Selectivity among Two Lectins: Probing the Effect of Topology, Multivalency and Flexibility of “Clicked” Multivalent Glycoclusters},
volume = {17},
year = {2011}
}
TY - JOUR
ID - 947554
AU - Cecioni, Samy - Faure, Sophie - Darbost, Ulrich - Bonnamour, Isabelle - Parrot-Lopez, Helene - Roy, Olivier - Taillefumier, Claude - Wimmerová, Michaela - Praly, Jean-Pierre - Imberty, Anne - Vidal, Sebastien
PY - 2011
TI - Selectivity among Two Lectins: Probing the Effect of Topology, Multivalency and Flexibility of “Clicked” Multivalent Glycoclusters
JF - Chemistry - A European Journal
VL - 17
IS - 7
SP - 2146–2159
EP - 2146–2159
PB - WILEY-VCH
SN - 09476539
KW - lectin
KW - glycocluster;SPR;ITC
N2 - We have designed a series of multivalent galactosylated glycoconjugates and studied their binding properties towards two lectins, from plant and bacterial origins, to determine their potential selectivity. The synthesis was achieved through copper(I)-catalysed azide-alkyne cycloaddition (CuAAC) under microwave activation between propargylated multivalent scaffolds and an azido-functionalised carbohydrate derivative. The interactions of two galactose-binding lectins from Pseudomonas aeruginosa (PA-IL) and Erythrina cristagalli (ECA) with the synthesized glycoclusters were studied by hemagglutination inhibition assays (HIA), surface plasmon resonance (SPR) and isothermal titration microcalorimetry (ITC). The results obtained illustrate the influence of the scaffold's geometry on the affinity towards the lectin and also on the relative potency in comparison with a monovalent galactoside reference probe.
ER -
CECIONI, Samy, Sophie FAURE, Ulrich DARBOST, Isabelle BONNAMOUR, Helene PARROT-LOPEZ, Olivier ROY, Claude TAILLEFUMIER, Michaela WIMMEROVÁ, Jean-Pierre PRALY, Anne IMBERTY a Sebastien VIDAL. Selectivity among Two Lectins: Probing the Effect of Topology, Multivalency and Flexibility of “Clicked” Multivalent Glycoclusters. \textit{Chemistry - A European Journal}. WILEY-VCH, 2011, roč.~17, č.~7, s.~2146–2159. ISSN~0947-6539. Dostupné z: https://dx.doi.org/10.1002/chem.201002635.
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