LPS structure influences protein secretion in Salmonella
enterica

J 2011

LPS structure influences protein secretion in Salmonella enterica

CRHANOVÁ, M.; M. MALCOVÁ; M. MAZGAJOVÁ; D. KARASOVÁ; A. ŠEBKOVÁ et al.

Základní údaje

Originální název

LPS structure influences protein secretion in Salmonella enterica

Autoři

CRHANOVÁ, M.; M. MALCOVÁ; M. MAZGAJOVÁ; D. KARASOVÁ; A. ŠEBKOVÁ; A. FUČÍKOVÁ; Zbyněk BORTLÍČEK; L. PILOUSOVÁ; K. KYROVÁ; M. DĚKANOVÁ a I. RYCHLÍK

Vydání

Veterinary Microbiology, Elsevier, 2011, 0378-1135

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.327

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/11:00052915

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.1016/j.vetmic.2011.04.018

UT WoS

000294095000015

Klíčová slova anglicky

Salmonella; LPS; rfaC; Protein secretion; Motility; SPI-1

Příznaky

Mezinárodní význam

Změněno: 12. 4. 2012 07:47, Mgr. Michal Petr

Anotace

V originále

In this study we have compared protein secretion in the wild type of S. Typhimurium and the rfaC mutant. We found out that the rfaC mutant was defective in protein secretion. In addition, the rfaC mutant was defective in its invasion into an IPEC-J2 porcine epithelial cell line and also in motility in semisolid agar. Consistent with this, reduced flagella numbers were observed in the rfaC mutant. In the rfaC mutant, there were no defects in flagellin expression as detected by western blot and immune electron microscopy which demonstrated equal amounts of flagellin in the cytoplasm of both the rfaC mutant and the wild-type S. Typhimurium. However, in the wild-type strain only, the flagellin was assembled to spatially restricted areas on the inner side of cytoplasmic membrane. The oligosaccharide core of LPS is therefore required for the assembly of flagella and T3SS secretion machinery followed by protein secretion.

Citovat

CRHANOVÁ, M.; M. MALCOVÁ; M. MAZGAJOVÁ; D. KARASOVÁ; A. ŠEBKOVÁ; A. FUČÍKOVÁ; Zbyněk BORTLÍČEK; L. PILOUSOVÁ; K. KYROVÁ; M. DĚKANOVÁ a I. RYCHLÍK. LPS structure influences protein secretion in Salmonella enterica. Veterinary Microbiology. Elsevier, 2011, roč. 152, 1-2, s. 131-137. ISSN 0378-1135. Dostupné z: https://doi.org/10.1016/j.vetmic.2011.04.018.

@article{948621,
   author = {Crhanová, M. and Malcová, M. and Mazgajová, M. and Karasová, D. and Šebková, A. and Fučíková, A. and Bortlíček, Zbyněk and Pilousová, L. and Kyrová, K. and Děkanová, M. and Rychlík, I.},
   article_number = {1-2},
   doi = {https://doi.org/10.1016/j.vetmic.2011.04.018},
   keywords = {Salmonella; LPS; rfaC; Protein secretion; Motility; SPI-1},
   language = {eng},
   issn = {0378-1135},
   journal = {Veterinary Microbiology},
   title = {LPS structure influences protein secretion in Salmonella enterica},
   volume = {152},
   year = {2011}
}

TY  - JOUR
ID  - 948621
AU  - Crhanová, M. - Malcová, M. - Mazgajová, M. - Karasová, D. - Šebková, A. - Fučíková, A. - Bortlíček, Zbyněk - Pilousová, L. - Kyrová, K. - Děkanová, M. - Rychlík, I.
PY  - 2011
TI  - LPS structure influences protein secretion in Salmonella enterica
JF  - Veterinary Microbiology
VL  - 152
IS  - 1-2
SP  - 131-137
EP  - 131-137
PB  - Elsevier
SN  - 03781135
KW  - Salmonella
KW  - LPS
KW  - rfaC
KW  - Protein secretion
KW  - Motility
KW  - SPI-1
N2  - In this study we have compared protein secretion in the wild type of S. Typhimurium and the rfaC mutant. We found out that the rfaC mutant was defective in protein secretion. In addition, the rfaC mutant was defective in its invasion into an IPEC-J2 porcine epithelial cell line and also in motility in semisolid agar. Consistent with this, reduced flagella numbers were observed in the rfaC mutant. In the rfaC mutant, there were no defects in flagellin expression as detected by western blot and immune electron microscopy which demonstrated equal amounts of flagellin in the cytoplasm of both the rfaC mutant and the wild-type S. Typhimurium. However, in the wild-type strain only, the flagellin was assembled to spatially restricted areas on the inner side of cytoplasmic membrane. The oligosaccharide core of LPS is therefore required for the assembly of flagella and T3SS secretion machinery followed by protein secretion.
ER  -

CRHANOVÁ, M.; M. MALCOVÁ; M. MAZGAJOVÁ; D. KARASOVÁ; A. ŠEBKOVÁ; A. FUČÍKOVÁ; Zbyněk BORTLÍČEK; L. PILOUSOVÁ; K. KYROVÁ; M. DĚKANOVÁ a I. RYCHLÍK. LPS structure influences protein secretion in Salmonella enterica. \textit{Veterinary Microbiology}. Elsevier, 2011, roč.~152, 1-2, s.~131-137. ISSN~0378-1135. Dostupné z: https://doi.org/10.1016/j.vetmic.2011.04.018.

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