Bilateral activation of glial cells in the trigeminal
subnucleus caudalis after unilateral infraorbital nerve injury

k 2011

Bilateral activation of glial cells in the trigeminal subnucleus caudalis after unilateral infraorbital nerve injury

STREJČKOVÁ, Lucie; Ilona KLUSÁKOVÁ a Petr DUBOVÝ

Základní údaje

Originální název

Bilateral activation of glial cells in the trigeminal subnucleus caudalis after unilateral infraorbital nerve injury

Autoři

STREJČKOVÁ, Lucie; Ilona KLUSÁKOVÁ a Petr DUBOVÝ

Vydání

10th European Meeting on Glial Cells in Health and Disease, Praha, 2011

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

astrocytes; microglia; contralateral side

Změněno: 3. 1. 2013 13:14, MVDr. Lucie Kubíčková, Ph.D.

Anotace

V originále

Peripheral nerve injury leads to activation of microglia and astrocytes in the spinal cord. The activated glial cells contribute to increased excitation of neurons and neuropathic pain induction and maintenance. The goal of our work was to investigate bilateral activation of astrocytes and microglia in the trigeminal subnucleus caudalis (TSC) following unilateral ligature of the infraorbital nerve (IONL). Wistar rats (males, 270g) were operated on unilateral IONL (n=12) and allowed to survive for 3 (n=6) and 7 (n=6) days. Naive (n=4) and sham-operated rats (n=4) were used as control. Immunofluorescence staining of brainstem sections for GFAP and OX-42 were used for detection of activated astrocytes and microglial cells, respectively. A part of brainstem sections was simultaneously incubated with anti-NeuN antibody to visualize neurons. The quantitative evaluation of GFAP and OX-42 staining was performed using image analysis system Lucia. In comparison to naive TSC, increased GFAP and OX-24 immunofluorescence brightness and its extent were found bilaterally in TSC of rats operated on IONL. Expression of GFAP and OX-24 indicating activated astrocytes and microglia was lower in contralateral than ipsilateral TSC. The TSC of sham-operated rats displayed also increased GFAP and OX-24 immunofluorescence brightness when compared with TSC from naive rats. Bilateral activation of astrocytes and microglia in the TSC following unilateral IONL leads to propagation of neuroinflammation and development of conditions for neuropathic pain induction.

Návaznosti

MSM0021622404, záměr

Název: Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS

Citovat

STREJČKOVÁ, Lucie; Ilona KLUSÁKOVÁ a Petr DUBOVÝ. Bilateral activation of glial cells in the trigeminal subnucleus caudalis after unilateral infraorbital nerve injury. In 10th European Meeting on Glial Cells in Health and Disease, Praha. 2011.

@proceedings{950799,
   author = {Strejčková, Lucie and Klusáková, Ilona and Dubový, Petr},
   booktitle = {10th European Meeting on Glial Cells in Health and Disease, Praha},
   keywords = {astrocytes; microglia; contralateral side},
   language = {eng},
   title = {Bilateral activation of glial cells in the trigeminal subnucleus caudalis after unilateral infraorbital nerve injury},
   url = {http://www.europeglia2011prague.cz/general-information/general-information.html},
   year = {2011}
}

TY  - CONF
ID  - 950799
AU  - Strejčková, Lucie - Klusáková, Ilona - Dubový, Petr
PY  - 2011
TI  - Bilateral activation of glial cells in the trigeminal subnucleus caudalis after unilateral infraorbital nerve injury
KW  - astrocytes
KW  - microglia
KW  - contralateral side
UR  - http://www.europeglia2011prague.cz/general-information/general-information.html
N2  - Peripheral nerve injury leads to activation of microglia and astrocytes in the spinal cord. The activated glial cells contribute to increased excitation of neurons and neuropathic pain induction and maintenance. The goal of our work was to investigate bilateral activation of astrocytes and microglia in the trigeminal subnucleus caudalis (TSC) following unilateral ligature of the infraorbital nerve (IONL). Wistar rats (males, 270g) were operated on unilateral IONL (n=12) and allowed to survive for 3 (n=6) and 7 (n=6) days. Naive (n=4) and sham-operated rats (n=4) were used as control. Immunofluorescence staining of brainstem sections for GFAP and OX-42 were used for detection of activated astrocytes and microglial cells, respectively. A part of brainstem sections was simultaneously incubated with anti-NeuN antibody to visualize neurons. The quantitative evaluation of GFAP and OX-42 staining was performed using image analysis system Lucia. In comparison to naive TSC, increased GFAP and OX-24 immunofluorescence brightness and its extent were found bilaterally in TSC of rats operated on IONL. Expression of GFAP and OX-24 indicating activated astrocytes and microglia was lower in contralateral than ipsilateral TSC. The TSC of sham-operated rats displayed also increased GFAP and OX-24 immunofluorescence brightness when compared with TSC from naive rats. Bilateral activation of astrocytes and microglia in the TSC following unilateral IONL leads to propagation of neuroinflammation and development of conditions for neuropathic pain induction.
ER  -

STREJČKOVÁ, Lucie; Ilona KLUSÁKOVÁ a Petr DUBOVÝ. Bilateral activation of glial cells in the trigeminal subnucleus caudalis after unilateral infraorbital nerve injury. In \textit{10th European Meeting on Glial Cells in Health and Disease, Praha}. 2011.

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