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@article{951814, author = {Šrůtková, Dagmar and Španová, Alena and Špano, Miroslav and Dráb, Vladimír and Schwarzer, Martin and Kozáková, Hana and Rittich, Bohuslav}, article_location = {Amsterdam}, article_number = {1}, doi = {http://dx.doi.org/10.1016/j.mimet.2011.06.014}, keywords = {Bifidobacterium longum; PCR; ARDR; RAPD; rep-PCR}, language = {eng}, issn = {0167-7012}, journal = {Journal of Microbiological Methods}, title = {Efficiency of PCR-based methods in discriminating Bifidobacterium longum ssp. longum and Bifidobacterium longum ssp. infantis strains of human origin}, volume = {87}, year = {2011} }
TY - JOUR ID - 951814 AU - Šrůtková, Dagmar - Španová, Alena - Špano, Miroslav - Dráb, Vladimír - Schwarzer, Martin - Kozáková, Hana - Rittich, Bohuslav PY - 2011 TI - Efficiency of PCR-based methods in discriminating Bifidobacterium longum ssp. longum and Bifidobacterium longum ssp. infantis strains of human origin JF - Journal of Microbiological Methods VL - 87 IS - 1 SP - 10-16 EP - 10-16 PB - Elsevier SN - 01677012 KW - Bifidobacterium longum KW - PCR KW - ARDR KW - RAPD KW - rep-PCR N2 - Four molecular-biological methods (PCRs, random amplified polymorphic DNA (RAPD) method using 5 primers, repetitive sequence-based (rep)-PCR with BOXA1R and (GTG)5 primers and amplified ribosomal DNA restriction analysis (ARDRA)) and biochemical analysis were compared for the classification of B. longum strains isolated from the faeces of breast-fed healthy infants (25), healthy adults (3) and 2 collection strains. These methods together with arabionose/ melezitose fermentation analysis clearly differentiated only the collection strains into B. longum ssp. longum and B. longum ssp. infantis at the subspecies level. On the other hand, ARDRA analysis differentiated the strains into the B. longum/infantis subspecies using the cleavage analysis of genus-specific amplicon with just one enzyme, Sau3AI. Moreover, we propose a classification of the collection strains into the subspecies level: B. longum CCM 3764 as B. longum ssp. longum CCM 3764, B. longum ATCC 17930 as B. longum ssp. infantis ATCC 17930. ER -
ŠRŮTKOVÁ, Dagmar, Alena ŠPANOVÁ, Miroslav ŠPANO, Vladimír DRÁB, Martin SCHWARZER, Hana KOZÁKOVÁ a Bohuslav RITTICH. Efficiency of PCR-based methods in discriminating Bifidobacterium longum ssp. longum and Bifidobacterium longum ssp. infantis strains of human origin. \textit{Journal of Microbiological Methods}. Amsterdam: Elsevier, 2011, roč.~87, č.~1, s.~10-16. ISSN~0167-7012. Dostupné z: https://dx.doi.org/10.1016/j.mimet.2011.06.014.
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