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@article{951814,
author = {Šrůtková, Dagmar and Španová, Alena and Špano, Miroslav and Dráb, Vladimír and Schwarzer, Martin and Kozáková, Hana and Rittich, Bohuslav},
article_location = {Amsterdam},
article_number = {1},
doi = {http://dx.doi.org/10.1016/j.mimet.2011.06.014},
keywords = {Bifidobacterium longum; PCR; ARDR; RAPD; rep-PCR},
language = {eng},
issn = {0167-7012},
journal = {Journal of Microbiological Methods},
title = {Efficiency of PCR-based methods in discriminating Bifidobacterium longum ssp. longum and Bifidobacterium longum ssp. infantis strains of human origin},
volume = {87},
year = {2011}
}
TY - JOUR
ID - 951814
AU - Šrůtková, Dagmar - Španová, Alena - Špano, Miroslav - Dráb, Vladimír - Schwarzer, Martin - Kozáková, Hana - Rittich, Bohuslav
PY - 2011
TI - Efficiency of PCR-based methods in discriminating Bifidobacterium longum ssp. longum and Bifidobacterium longum ssp. infantis strains of human origin
JF - Journal of Microbiological Methods
VL - 87
IS - 1
SP - 10-16
EP - 10-16
PB - Elsevier
SN - 01677012
KW - Bifidobacterium longum
KW - PCR
KW - ARDR
KW - RAPD
KW - rep-PCR
N2 - Four molecular-biological methods (PCRs, random amplified polymorphic DNA (RAPD) method using 5 primers, repetitive sequence-based (rep)-PCR with BOXA1R and (GTG)5 primers and amplified ribosomal DNA restriction analysis (ARDRA)) and biochemical analysis were compared for the classification of B. longum strains isolated from the faeces of breast-fed healthy infants (25), healthy adults (3) and 2 collection strains. These methods together with arabionose/ melezitose fermentation analysis clearly differentiated only the collection strains into B. longum ssp. longum and B. longum ssp. infantis at the subspecies level. On the other hand, ARDRA analysis differentiated the strains into the B. longum/infantis subspecies using the cleavage analysis of genus-specific amplicon with just one enzyme, Sau3AI. Moreover, we propose a classification of the collection strains into the subspecies level: B. longum CCM 3764 as B. longum ssp. longum CCM 3764, B. longum ATCC 17930 as B. longum ssp. infantis ATCC 17930.
ER -
ŠRŮTKOVÁ, Dagmar, Alena ŠPANOVÁ, Miroslav ŠPANO, Vladimír DRÁB, Martin SCHWARZER, Hana KOZÁKOVÁ and Bohuslav RITTICH. Efficiency of PCR-based methods in discriminating Bifidobacterium longum ssp. longum and Bifidobacterium longum ssp. infantis strains of human origin. \textit{Journal of Microbiological Methods}. Amsterdam: Elsevier, 2011, vol.~87, No~1, p.~10-16. ISSN~0167-7012. Available from: https://dx.doi.org/10.1016/j.mimet.2011.06.014.
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